BACTROX HYPOXIC CHAMBERS OPERATION MANUAL MODEL: BACTROX Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113 EMAIL: tech@Shellab.com INTERNET: http://www.Shellab.
__________________________________________________________________________ Contents 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. OPERATION MANUAL .................................................................................................. 1 ORIENTATION ............................................................................................................... 3 RECEIVING AND INSPECTION ..............................................................................
__________________________________________________________________________ IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY. Your satisfaction and safety require a complete understanding of this unit, including its proper function and operational characteristics. Be sure operators are given adequate training before attempting to put the unit in service. NOTE: This equipment must be used only for its intended application; any alterations or modifications will void your warranty. 1.
__________________________________________________________________________ Figure 1: Sleeve/Cuff Assembly The Sleeve/Cuff Assembly consists of a neoprene sleeve, a plastic cuff ring, O-Ring, and a soft rubber cuff. Unit comes with Latex Gloves and Cuffs. Nitrile Gloves and Cuffs may be ordered from the Parts List. The assembly allows gloveless chamber operation. Replaceable Arm Port door O-ring seals.
__________________________________________________________________________ Oxygen Sensor and Box Inner Airlock Door Incubator Figure 5. Pass Box The PASS BOX provides ability to take material into and out of the chamber. PASS BOX can be operated either automatically or manually. The PASS BOX shelf allows for materials to be conveniently rolled from the PASS BOX into the chamber. Figure 6: Incubator Bottom Shelf Spacer Three (3) are provided with the Bactrox.
__________________________________________________________________________ 2. RECEIVING AND INSPECTION 2.1 2.2 2.3 The carrier, when accepting shipment, also accepts responsibility for safe delivery and is liable for loss or damage claims. On delivery, you must inspect for visible exterior damage. Note and describe on the freight bill any damage found and enter your claim on the form the carrier supplies. Inspect for concealed loss or damage on the unit itself, both interior and exterior.
__________________________________________________________________________ WARNING: Follow these safety precautions regardless of the cleaning procedure. Failure to do so may cause serious injury or death. Always disconnect the unit from the electrical service when cleaning. Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit to the power supply. NOTICE: Follow these precautions regardless of cleaning procedure.
__________________________________________________________________________ 4. CONTROL PANEL OVERVIEW 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.11 4.12 4.13 POWER: The I/O (ON/OFF) switch controls all of the power for the chamber and must be in the I/ON position before any systems are operational. Both “SET TEMPERATURE” “SETO2 %” and “SET CO2 %” displays will illuminate when the power switch is in the ON position.
__________________________________________________________________________ 5.3.2 Reading & Understanding Your CO2 Tank Levels: Pure CO2 is in a liquid state in the tank, and a constant vapor pressure is generated in the tank above the liquid level. The CO2 is drawn off of the top as a gas. It is normal for the high pressure gauge on your regulator to start out reading 800 to 1000 PSI with a full tank. The same vapor pressure is maintained as long as any liquid is left in the tank.
__________________________________________________________________________ point temperature. Allow the incubator at least 24 hours to stabilize. Then re-calibrate the digital display to your reference thermometer, follow the calibration instructions. This incubator was calibrated at the factory at 370C. 7.2 Setting Incubator Safety: First, set the control to the desired incubator temperature and then the INCUBATOR SAFETY to its maximum position. Allow 24 hours for stabilization before proceeding. 7.
__________________________________________________________________________ Bactrox but is readily available from your dealer. Follow the instructions provided with each Fyrite instrument carefully to ensure correct and accurate readings. Fyrite Quick Overview Press button on top of Fyrite canister to release CO2 concentration. RELEASE BUTTON and tip canister to the side to ensure all fluid is released from the top of the canister. Return canister to the upright position.
__________________________________________________________________________ 9.5 TWO POINT CALIBRATION PROCEDURE 9.5.1 The two point calibration procedure requires a terminal emulator program. Shel Lab recommends the free, open source emulator Tera Term. 9.5.2 Tera Term can be downloaded for free from the developer’s project page http://ttssh2.sourceforge.jp/index.html.en. Follow the instructions to download and install the emulator. 9.5.
__________________________________________________________________________ 9.5.17 To enter a low calibration, type in LC2= (value of certified calibration gas) in uppercase in the TERATERM program. The display will show LC2=( value of certified calibration gas) 9.5.18 Unhook the calibration gas and reconnect the Calibration O2 pump box again and allow at least 5 minutes for the sensor to stabilize. This will show the curve is still accurate over the full range. 9.5.19 The display should return to 20.
__________________________________________________________________________ 10.5 Initial Control Settings: Each Bactrox comes preset from the factory at 37C and the O2 and CO2 set to off. You should allow the incubator to heat up and stabilize for 24hrs. Turn the SET OVERTEMPERATURE control clockwise until it stops so that it does not activate during the initial warm up. 10.6 Calibrating the Controls for Temperature or CO2: 10.6.
__________________________________________________________________________ 12.3.1 Locate the “START PASS BOX PURGE” switch which should be in the bottom OFF position. NOTE: Do not hold Auto Cycle Start Switch down. Simply press the button and let go to activate. 12.3.2 Press the “START PASS BOX PURGE” switch once to initiate the cycle. The PASS BOX will purge nitrogen thru the PASS BOX for 45 seconds. The PASS BOX PURGING light will alternately flash on and off, indicating the cycle is in progress.
__________________________________________________________________________ 16. Day two – remove beaker in the chamber and replace with the second beaker of Anatox (also new). Day three – reactivate the first beaker of Anatox by heating at 160C for 2 hours. Then replace the beaker in the chamber with this reactivated beaker. Day four – six months – continue switching the reactivated beakers daily until six months is complete, then discard the old Anatox and repeat the procedure again.
__________________________________________________________________________ CAUTION: Extreme caution must be exercised any time access is made into areas housing electrical components. Repair, replacement or adjustment of components in these areas must only be done by qualified technicians familiar with electrical circuitry and the operation of the hypoxic chamber. ATTENTION: Des Précautions extrèmes sont requises à chaque fois que vous accédez à des endroits qui abritent les composantes électriques.
__________________________________________________________________________ Problem Possible Cause Solution Amount of Water Collected in the The amount of water collected in Dehumidifier: the dehumidifier depends on the number and types of samples in the chamber. The typical amount collected is around 50 cc each day of chamber use. Excessive moisture buildup in A. Thermoelectric condensate A. Replace fan. chamber. controller fan is not operating. B. Ambient room temperature too low. B1.
__________________________________________________________________________ 18. BACTROX OPERATION TIPS Gas Source: Sheldon Manufacturing, Inc. recommends an two separate tanks of 100% CO, , and 100% Nitrogen for Hypoxic applications. These mixtures are available marked “NON-FLAMMABLE”. Two national sources of gas are AIRGAS, PRAXAIR, and Air Liquid.
__________________________________________________________________________ 19. UNIT SPECIFICATIONS Model Number Interior Dimensions WxDxH (Inches) BACTROX, BACTROX-2 Model Number BACTROX, BACTROX-2 Exterior Dimensions WxDxH (Inches) Chamber Capacity 48.5 x 31.0 x 32.6 300 plates Temperature Range (Control) Ambient + 8 to 60C (+/- 0.1C) Shipping Weight Unit Weight Temperature Uniformity O2 Range CO2 Range +/- 0.
__________________________________________________________________________ DIAGRAMS WIRING DIAGRAM BACTROX (9851358) BLACK 14G EMI FILTER 2800502 INLET 4200505 WHITE 14G BLACK 14G WHITE 14G GREEN LIGHTED POWER SWITCH 7850570 BLACK 14G TB4#23 WHITE 14G CHAMBER FAN 4880550 TB4#19 TB1#1 BLACK 14G WHITE 14G AIR PUMP 6700558 TB4#21 BLACK 14G GREY 14G 2 1 SOLID STATE RELAY 7030533 4 60W 120V SENSOR 8100500 HEAT LIGHT 4650554 WHITE 14G TB2#8 TB1#4 60W 120V WHITE 14G TB1#6 OTP LIGHT 465
__________________________________________________________________________ WIRING DIAGRAM BACTROX-2 (9851363) BLACK 14G EMI FILTER 2800502 INLET 4200505 WHITE 14G BLACK 14G WHITE 14G WHITE 14G GREEN LIGHTED POWER SWITCH 7850570 BLACK 14G TB4#23 WHITE 14G CHAMBER FAN 4880551 TB4#19 TB1#1 BLACK 14G WHITE 14G AIR PUMP 6700559 TB4#21 BLACK 14G GREY 14G 2 1 SOLID STATE RELAY 7030533 4 SENSOR 8100500 WHITE 14G TB2#8 TB1#4 2350502 WHITE 14G TB1#6 OTP LIGHT 4650553 60W 120V RED HT 14G O
__________________________________________________________________________ TTB1-16 TTB1-15 TTB1-14 TTB1-13 TTB1-12 TTB1-11 TTB1-9 TTB1-10 TTB1-7 TTB1-8 TTB1-6 TEMPERATURE SENSOR BROWN 24G BLACK 24G 8000547 TO TEMP DISPLAY BUSS CONNECTION TTB1 BLACK 24G WHITE 24G BLACK 18G BLACK 18G BLACK 24G ALARM CONTACTS RED 18G RED 18G RED 18G 4 LOOP CONTROL BOARD SL# 1750972 EARTH GRD J3 (NEUT) J2 (HOT) J1 LOAD 1 J4 LOAD 3 J6 LOAD 4 J7 24VDC POWER SUPPLY 6750516 8000542 80K OHMS TB5-1 TB5
__________________________________________________________________________ TTB1-16 TTB1-15 TTB1-14 TTB1-13 TTB1-12 TTB1-11 TTB1-9 TTB1-10 TTB1-7 TTB1-8 TTB1-6 TEMPERATURE SENSOR BROWN 24G BLACK 24G 8000547 TO TEMP DISPLAY BUSS CONNECTION TTB1 BLACK 24G WHITE 24G BLACK 18G BLACK 18G BLACK 24G ALARM CONTACTS RED 18G RED 18G RED 18G 4 LOOP CONTROL BOARD SL# 1750972 EARTH GRD J3 (NEUT) J2 (HOT) J1 LOAD 1 J4 LOAD 3 J6 LOAD 4 J7 24VDC POWER SUPPLY 6750516 8000542 80K OHMS TB5-1 TB5
__________________________________________________________________________ CONTROL PANEL BACTROX (9851360) TEMPERATURE SENSOR 8100500 RED 18G BLACK 18G WHITE 24G 3 4 1 SOLID STATE RELAY 7030533 2 RED LED LIGHT BAR 4650547 TB4#19 TB4#20 TB4#21 TB4#22 TB4#23 TB4#23 TB1#1 WHITE TB1#2 WHITE WHITE TB1#3 WHITE TB1#4 WHITE TB1#5 TB1#6 TB2#7 WHITE WHITE HT TB2#8 WHITE TB2#9 WHITE TB2#10 TB2#11 TB2#12 TB3#13 TB3#14 TB3#15 TB3#16 TB3#17 TB3#18 BLACK BLACK BLACK BLACK BLACK ORANGE BLUE GREY WHITE WHIT
__________________________________________________________________________ CONTROL PANEL BACTROX-2 (9851365) TEMPERATURE SENSOR 8100500 RED 18G BLACK 18G BLACK 24G 3 4 1 SOLID STATE RELAY 7030533 2 RED LED LIGHT BAR 4650547 TB4#19 TB4#20 TB4#21 TB4#22 TB4#23 TB4#23 TB1#1 WHITE WHITE TB1#2 WHITE TB1#3 WHITE TB1#4 WHITE TB1#5 TB1#6 TB2#7 WHITE WHITE HT TB2#8 WHITE TB2#9 WHITE TB2#10 TB2#11 TB2#12 TB3#13 TB3#14 TB3#15 TB3#16 TB3#17 TB3#18 BLACK BLACK BLACK BLACK BLACK ORANGE BLUE GREY WHITE WH
__________________________________________________________________________ TO TB4#23 TO TB1#3 WHITE 14G BLACK 14G TO OTP CONTROL SENSOR GREEN/YELLOW 14G RED HIGH TEMP 14G TO OTP CONTROL WHITE HIGH TEMP 14G TO TB2#8 INCUBATOR & FAN BACTROX (9851361) FAN PIGTAIL CORD 1800536 120V 65W 2350502 CHAMBER FAN 4880550 120V 65W 2350502 INCUBATOR Bactrox Operations Manual 27
__________________________________________________________________________ TO TB4#23 TO TB1#3 WHITE 14G BLACK 14G TO OTP CONTROL SENSOR GREEN/YELLOW 14G RED HIGH TEMP 14G TO OTP CONTROL WHITE HIGH TEMP 14G TO TB2#8 INCUBATOR & FAN BACTROX-2 (9851366) FAN PIGTAIL CORD 1800536 120V 65W 2350502 CHAMBER FAN 4880551 120V 65W 2350502 INCUBATOR Bactrox Operations Manual 28
__________________________________________________________________________ TB7#11 TB8#24 TO TTB1#2 TO TTB1#1 CHILLER & OUTLET BACTROX (9851362) B B R Bactrox Operations Manual B R R 29 BLACK 14G GREEN/YELLOW 14G WHITE 14G RED 18G BLACK 18G ALL END STRIP .
__________________________________________________________________________ TB7#11 TB8#24 TO TTB1#2 TO TTB1#1 CHILLER & OUTLET BACTROX-2 (9851367) B B R BLACK 14G GREEN/YELLOW 14G WHITE 14G RED 18G BLACK 18G ALL END STRIP .
__________________________________________________________________________ PLUMBING DIAGRAM 20.
__________________________________________________________________________ 21. BACTROX SPARE PARTS LIST DESCRIPTION CHECK VALVE FILTER CONTROL - OTL SAFETY CONTROL - PLC CONTROL - TEMPERATURE CONTROL PROBE - OTP SAFETY ELEMENT GASKET 9 X 9 SOFT LT.
