Users Manual Part 1

4 - 4 Operator’s Manual
4 Understanding the System Principles
4.3 Hemoglobin Concentration Measurement
4.3.1 A Test Model Using the Colorimetric Method
Figure 4-4 Colorimetric method
According to the Lambert-Beer Principle, when a beam of monochromatic light passes through a
well-proportioned non-scattering light-absorbing solution, the absorbance A is proportional to the
product of the thickness L and the concentration C. The sample in the HGB channel acts as the light
Eos% Eosinophil
percentage
%
Lym# Lymphocyte count Lym# = WBC x Lym% 10
9
/L
Lym% Lymphocyte
percentage
%
Mon# Monocyte count Mon# = WBC x Mon% 10
9
/L
Mon% Monocyte
percentage
%
Paramete
rs
Name Formula/Test Methods Unit
Eos% =
Particles in the Eos reagion in the DIFF channel
Sum of all particles in DIFF channel except those in Ghost reagion
×100%
Lym% =
Particles in the Lym reagion in the DIFF channel
Sum of all particles in DIFF channel except those in Ghost reagion
×100%
Mon% =
Particles in the Mon reagion in the DIFF channel
Sum of all particles in DIFF channel except those in Ghost region
× 100%