Instruction manual

5. Application Operation
5-62
RF-20A/20Axs
5.5.2 Overview of Spectrum Measurement
Spectrum measurement is carried out in accordance with the flow shown below. Details of each step are
explained on the reference pages.
 Setting the Spectrum Scanning Conditions
 Setting Other Measurement Conditions
 Measuring the Mobile Phase Spectrum
 Measuring the Spectrum of a Sample
 Outputting Spectrum Data
Operation Details Command Page
Selecting whether the excitation side or the emission side is to be
scanned
Control settings group
[SPC TYPE]
P.5-63
Setting the scan start wavelength and scan end wavelength
Control settings group
[EX SCAN] or [EM SCAN]
P.5-64
Wavelength setting
When scanning the excitation side, set a wavelength that emits
fluorescent light for the emission side, and when scanning the
emission side, set a wavelength that absorbs excitation light for the
excitation side.
Control settings group
[ch1 EX*** EM***]
P.5-65
Scan speed setting
Control settings group
[SCAN SPEED]
P.5-66
Operation Details Command Page
Setting the measurement range (setting sensitivity and gain)
Parameter settings group
[SENS] or [GAIN]
P.5-67
P.5-68
Setting the flow cell temperature (for RF-20Axs)
Parameter settings group
[CELL TEMP]
P.5-69
Operation Details Command Page
Setting the scan file for background use
(SCAN FILE: 0)
Control settings group
[SCAN FILE]
P.5-70
Measuring the spectrum of the mobile phase key P.5-72
Operation Details Command Page
Setting the scan file for sample use
(SCAN FILE: 1 or 2)
Control settings group
[SCAN FILE]
P.5-72
Measuring the spectrum of a sample key P.5-72
Operation Details Command Page
Setting the plot speed
Control settings group
[PLOT SPD]
P.5-73
Selecting the spectrum file to be output
Control settings group
[SCAN FILE]
P.5-73
Executing plotting
Control settings group
[SPC PLOT]
P.5-73