Instruction manual

5. Application Operation
5-70
RF-20A/20Axs
5.5.10 Setting the Scan File
This instrument can save two sample spectra and one background spectrum.
First of all, set the file number of the file in which the scan data is to be saved. Details of the file number
settings are given below.
In spectrum scanning, first the mobile phase is scanned and the data acquired is saved in file number [0].
After that the sample is scanned and the data from this scan is saved in file number [1] or [2].
After measurement has ended, by outputting after subtracting the mobile phase scan data from the sample
scan data, the spectrum of the sample can be ascertained (differential spectrum measurement).
NOTE
• No subtraction is involved in display and output during measurement.
• When scan data is output after measurement, the background spectrum is subtracted from the sample
spectrum and the result is output. First of all, in the state where the mobile phase has filled the flow cell,
specify [0] as the file number and execute a scan.
• If differential spectrum measurement is not necessary, specify file number [0] at sample measurement.
1
Press .
The initial screen will be displayed.
2
Press twice.
[CONTROL] will be displayed.
File Number Setting
0 For background spectra
1
For sample spectra
2
EX200nm EM300nm
Xe 1000.00
CONTROL
Enter to Select