Instruction manual

5. Application Operation
5-72
RF-20A/20Axs
5.5.11 Measuring Procedure
1
Press while the flow cell is full of mobile
phase.
Spectrum scanning will start.
During spectrum scanning the screen to the right
is displayed and the wavelength in the first line is
updated sequentially.
When spectrum scanning has proceeded from the
start wavelength to the end wavelength, scanning
stops and the initial screen is displayed.
Initial screen
2
Pour in the sample.
3
Stop flow into the cell at the point where the target
peak has been eluted out.
4
Once the baseline has stabilized, display [SCAN
FILE] in the control settings group and press
or .
5
Press .
Spectrum scanning will start.
NOTE
• Do not change the scanning conditions (start wavelength, end wavelength, scan speed, etc.) between the
background ([SCAN FILE] = 0) and the sample ([SCAN FILE] = 1, 2). This will mean that correct spectrum
data cannot be obtained.
• In gradient analysis, perform a gradient run without injecting the sample. Scan the background, stopping
pumping when the peak is eluted out.
254nm 0.000 AU
SCANNING
Wavelength indication
EX200nm EM300nm
Xe 1000.00
SCAN FILE 0
0:BG 1:S1 2:S2