Instruction manual

7.6 System Validation
7-37
RF-20A/20Axs
7
 Checking and Preparing the LC System
1
Check the connection status of each unit. Details
on the connection of each unit are given in the
units' instruction manuals. Connect the input cable
of the Chromatopac through the relay terminal
block provided with the Chromatopac to the
integrator terminal of the detector with the signal
cable (accessory with the detector).
∗ If the system normally uses a Chromatopac or
LC workstation, the connections used for
regular analysis will be satisfactory.
2
Check the plumbing of the LC system. In
particular, ensure that the plumbing from the
autosampler outlet to the column inlet, and that
from the column outlet to the detector inlet uses
tubing with an internal diameter of no greater than
0.3 mm and no longer than 300 mm in order to
keep the volume outside the column as small as
possible.
Fig. 7.8
3
Clean the system flow lines using one of the
procedures described below.
Before cleaning the flow lines, remove the column
from the system, and connect the column inlet
tubing to the column outlet tubing with a coupling
1.6C ("Fig. 7.8").
For a new system:
Clean the flow lines first with 2-propanol, then with
water. In each case, pass the liquid through the
flow lines for 10 minutes, at a rate of 2 mL/min.
For a system in use that uses a mobile phase with
a low dielectric constant, such as hexane:
Clean the flow lines first with 2-propanol, then with
water. In each case, pass the liquid through the
flow lines for 10 minutes, at a rate of 2 mL/min.
Fig. 7.9
Detector
Autosampler
Coupling 1.6C
Detector
Autosampler
Tubing
Tubing
Column