Instruction manual

7. Hardware Validation
7-38
RF-20A/20Axs
For a system that has been using a mixture of a
water solution and an organic solvent as mobile
phase, or water plus an organic solvent miscible
with water (methanol, acetonitrile, etc.):
Clean the flow lines with water. Pass water
through the flow lines for 10 minutes, at a rate of
2 mL/min.
4
When cleaning is finished, pour mobile phase
(mixture of methanol and water (9/1, (v/v)) into the
reservoir bottle, and reconnect the column with
the LC system ("Fig. 7.9"). Pour methanol into the
reservoir bottle as a rinse liquid to purge the
autosampler.
 Check Procedure
Pump's display screen
1
Set the pumping flow rate to 1 mL/min.
Refer to the pump's instruction manual for the
setting procedure.
Column oven's display screen
2
Set the column oven temperature to 40 °C.
Refer to the column oven's instruction manual for
the setting procedure.
3
Press on the pump panel, and
on the column oven panel. Pumping and
temperature regulation will start. Verify that liquid
flows through the detector outlet tubing, and that
there are no leaks from any of the connections.
1.000mL MAX 20.0
0.0MPa MIN 0.0
SET TEMP 40
Input 4 - 85