Instruction manual

7. Hardware Validation
7-44
RF-20A/20Axs
Detector's display screen
4
Set the detector parameters.
^ "Parameter Settings for Isocratic System
Validation" P.7-45
Refer to the detector's instruction manual for the
setting procedure.
5
Set the autosampler parameters.
^ "Parameter Settings for Isocratic System
Validation" P.7-45
Refer to the autosampler's instruction manual for
the setting procedure.
6
Set the data processor parameters.
^ "Parameter Settings for Isocratic System
Validation" P.7-45
Refer to the data processor's instruction manual
for the setting procedure.
7
Monitor the baseline.
When the baseline has stabilized, press
on the detector, then inject 10 μL of mobile phase,
and verify that no peaks are observed.
8
Inject 10 μL of the test sample five times, and
analyze the data obtained.
9
From the peak data obtained from the five
analyses, derive the relative standard deviation
(coefficient of variation (C.V.)) for: retention time
and peak area ("Fig. 7.13").
Fig. 7.13
EX360nm EM450nm
Xe 0.00
X = (X1 + X2 ... Xn − 1 + Xn) / n
n: Number of analyses
X1 ... Xn: Retention time (or areas) of each peak
X:
Average
SD: Standard deviation
RSD: Relative standard deviation
C.V.: Coefficient of variation
RSD (C.V.) value = (SD/X
) × 100
SD =
n
Σ (Xi−X)
2
n−1
i=1