Instruction manual

7.6 System Validation
7-45
RF-20A/20Axs
7
 Parameter Settings for Isocratic System Validation
The parameters to be set for the various components when validation analysis of an isocratic system is
performed are given below.
• Pump.......................................
Flow rate 1 mL/min
B. CONC 90 %
P. Ma x 2 0 . 0 M P a
• Column oven...........................
Oven temperature 40 °C
• Time program..........................
7.00 STOP
• Autosampler............................
RINSE VOLUME 200 μL
RINSE SPEED 35 μL/s
SAMPLING SPEED 5 μL/s
RINSE MODE 0 (No needle rinsing)
• Detector ..................................
EX (excitation wavelength) 360 nm
EM (emission wavelength) 450 nm
RESPONSE 3 (0.5 sec)
SENS 2 (MED)
GAIN 1 (
×1)
CELL TEMP 30 °C (RF-20Axs only)
If the peak height exceeds the measuring range, change the SENS and GAIN
settings to achieve a lower sensitivity.
• Data processor........................
WIDTH 5
DRIFT 0
T. DB L 0
ATTEN 10
SLOPE 500
MIN. AREA 100000
STOP. TM 7
SPEED 5
CHECK CRITERIA: When temperature fluctuations in a room are within ± 1 °C, the
check criterion values for the relative standard deviation
(coefficient of variation) of each item are as follows.
Retention time: 0.5 % max.
Peak area: 2.0 % max. for anthracene