Instruction manual

9.4 Introduction to the HPLC System
9-47
RF-20A/20Axs
9
9.4.3 Example of Autosampler System (2)
The CBM-20A system controller can control a maximum of eight LC components (twelve LC components as
an option).
Use the same type of pumps for a high-pressure gradient system.
1 Mobile phase is drawn out of the reservoir bottle and pumped through the tubing by the pump.
2 The degasser removes dissolved air from the mobile phase, preventing air bubbles and consequent rise,
drift or other baseline irregularities caused by dissolved air.
3 The pump unit sends the mobile phase through the autosampler, column and detector, in that order, and
finally into the waste container.
4 The mixer enhances the mixing efficiency of the mobile phases.
5 The autosampler automatically injects the sample into the flow lines. By adding a rack changer, it is
possible to automatically change the autosampler racks.
6 In the column, the components are separated by means of the mutual interactions of the mobile phase
and the column packing (stationary phase).
7 The detector detects the components eluted from the column, and sends the signal data to a
Chromatopac or PC.
8 Mobile phase from the detector drains into the waste container.
9 The CBM-20A system controller can control a maximum of eight LC components (twelve LC components
as an option) including a maximum of four pump units.
1
2
3
3
5
9
7
6
8
4
 Solvent Flow  Function of Components
Degasser
Reservoir bottle
Pump unit
1
2
3
Mixer
4
Autosampler
5
Column
6
Detector
7
Waste container
8