Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Biological Laboratories
Siemens Industry, Inc. 95
Biosafety
Cabinet
Testing
(Continued)
Exception: Average intake velocity can also be measured directly at the work access
opening using a calibrated total capture air flow hood to measure the air volume
entering the cabinet, and dividing this measurement by the area of the work access
opening to determine the average face velocity.
(D) A quantitative aerosol challenge test shall be performed on each high-efficiency
particulate air filter. The test must be capable of detecting penetrations exceeding
0.005% of particles 0.3 micrometers or larger while the cabinet is in normal operation.
Any measurement exceeding 0.03% penetration shall establish a failure of the test.
Appendix A contains a recommended high efficiency particulate air filter test protocol.
(E) The ability of the hood to maintain an inward flow as required by subsection (e)
above shall be demonstrated using smoke tubes or other suitable qualitative
methods.
(3) The ventilation test requirements for Class III biological safety cabinets are as
follows:
(A) The airflow through the Class III biological safety cabinet shall be determined by
measuring the exhaust velocity at the exhaust port. Total air volume is calculated by
the following equation: (exhaust velocity) X (area of exhaust port) = total air volume.
The air change rate for a class III biological safety cabinet shall be a minimum of 1 air
change in 3 minutes or airflow required to maintain flammable gases/vapors below
20% of the LEL whichever is greater. The measurement of the negative pressure
inside the cabinet shall be made with a calibrated gauge. The accuracy of the gauge
shall be +5% at the required 0.5 inches of water gauge.
(B) A quantitative aerosol challenge test shall be performed on exhaust HEPA filters.
The test must be capable of detecting penetrations exceeding 0.005% of particles,
0.3 micrometer or larger while the cabinet is in normal operation. Any measurement
exceeding 0.03% penetration shall establish a failure of the test. Appendix A contains
a recommended high efficiency particulate air filter test protocol.
(g) Special requirements.
(1) All test or maintenance activities requiring access to potentially contaminated
interior spaces of the cabinet shall be performed after appropriate decontamination.
(2) A warning placard shall be placed on the front of the cabinet requiring
decontamination prior to opening any service panel or other interior access.
(3) Where biological safety cabinets are attached to external duct systems with a
blower and the cabinet system also contains a blower, or where the cabinet uses an
external blower, an audible and visual alarm system to alert the user indicating the
loss of exhaust flow in the external duct shall be used. Biological safety cabinets
which are served with a canopy or thimble connected exhaust system shall have a
ribbon streamer or like device attached to the edge of the canopy or thimble to
indicate the direction of flow and are exempt from the requirement for flow alarms.