Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 126
Topic Requirement(s) Commentary
Periodic
Inspection
and Testing -
Canada
(Continued)
•
with the room under continuous pressure, apply bubble solution to areas to be
tested (joints, corners, sealed penetrations, etc.) or, by using audible leak location
method, locate audible leaks (electronic sound detection equipment option);
• identify places where bubbles are found;
• after repair of leak, retest as required.
5.3 Air Handling Systems
Various components of a containment room's air handling system require
commissioning. Manufacturers' requirements for airflows for BSCs must be met.
Integrity testing of HEPA filters must be performed to ensure that they do not contain
leaks in the filter media, the gasketing or the seal to the filter housing. This filter
housing test is performed by challenging with a known particulate concentration and
scanning for percentage of penetration downstream of the filter. Ductwork systems
should be pressure decay tested to confirm that specified leakage rates are not
exceeded. The American Society of Mechanical Engineers (ASME) Standard N510
Testing of Nuclear Air Treatment Systems, 1989, reaffirmed 1995
(2)
, gives procedures
for testing the leak-tightness of ducts and plenums. The performance of room pressure
control systems must meet the design intent (e.g., negative pressures
must be maintained).
The following testing requirements and acceptance criteria must be satisfied for
certification of laboratories.
• Air Handling Systems - Containment Level 3 (CL3) Testing Requirements:
1. Classes I and II BSCs to be tested in situ in accordance with NSF/ANSI 49-
2002
(3)
or CSA Z316.3-95
(4)
.
2. Class III BSCs to be tested in situ in accordance with the Laboratory Safety
Monograph, NIH 1979
(5)
and BS EN 12469-2000
(6)
.
3. Interlocks (that is, Class II Type B2 BSC internal cabinet supply fan and
exhaust fan) to be tested in accordance with NSF/ANSI 49:2002
(3)
to ensure
that internal supply fan shuts off whenever exhaust fan fails.
4. Alarms to be tested for detection of BSC and/or exhaust fan failure by
simulation of alarm conditions.
5. Integrity of HEPA filters installed into supply as method of backdraft protection
and exhaust ductwork to be tested in situ by particle challenge testing using
the scanning method according to IEST-RP-CC-006.2 (section 6.2)
(7)
.
Acceptance criteria: particle penetration not to exceed 0.01%. Small in-line
filters need not be in situ scan tested – maintenance program to include visual
inspection and regular replacement.
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