Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 128
Topic Requirement(s) Commentary
Periodic
Inspection
and Testing -
Canada
(Continued)
• Air Handling Systems - Containment Level 4 (CL4) Testing Requirements:
1. Classes I and II BSCs to be tested in situ in accordance with NSF/ANSI 49-
2002(3) or CSA Z316.3-95 (4).
2. Class III BSCs to be tested in situ in accordance with the Laboratory Safety
Monograph, NIH 1979(5) and BS EN 12469-2000(6).
3. Interlocks (that is, Class II Type B2 BSC internal cabinet supply fan and
exhaust fan) to be tested in accordance with NSF/ANSI 49:2002(3) to ensure
that internal supply fan shuts off whenever exhaust fan fails.
4. Alarms to be tested for detection of BSC and/or exhaust fan failure by
simulation of alarm conditions.
Integrity of HEPA filters installed into supply as method of backdraft
protection and exhaust ductwork to be tested in situ by particle challenge
testing using the scanning method according to IEST-RP-CC-006.2 (section
6.2)(7). Acceptance criteria: particle penetration not to exceed 0.01%. Small
in-line filters need not be in situ scan tested – maintenance program to
include visual inspection and regular replacement.
5. Integrity of HEPA filter housings with inlet and outlet bubble tight dampers
installed into supply ductwork, where HEPA filters are used as backdraft
protection, and exhaust ductwork to be tested in situ by pressure decay
testing in accordance with ASME N510(2).Acceptance criteria: rate of air
leakage not to exceed 0.1% of housing vol/min at 1000 Pa (4 in. w.g.)
minimum test pressure. This test is not a mandatory requirement for
recertification if no physical modification or changes have been made. If
modifications have been performed then the laboratory supervisor, in
consultation with the Biological Safety Officer/ Institutional Biosafety
Committee, shall determine the degree of change and whether this test is
subsequently required.
6. Item 7 is not required for CL3 labs.
7. Supply ductwork, where backdraft protection is required on supply, and
exhaust air ductwork located between containment perimeter and HEPA filter
or bubble tight backdraft damper to be tested in situ by pressure decay
method in accordance with ASME N510(2).Acceptance criteria: rate of air
leakage not to exceed 0.1% duct vol/min at 1000 Pa (4 in. w.g.) minimum test
pressure.
8. Recommendation: Supply and exhaust air ductwork between containment
perimeter and HEPA filter or bubble tight backdraft damper to be tested in situ
by pressure decay method in accordance with ASME N510(2). Acceptance
criteria: rate of air leakage not to exceed 0.1% duct vol/min at 1000 Pa (4 in.
w.g.) minimum test pressure.
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