Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 62
Topic Requirement(s) Commentary
Biosafety
Level 4
(Continued)
National Sanitation Foundation, NSF 49 -2008, Biosafety Cabinetry: Design,
Construction, Performance and Field Certification
3 Definitions
3.3. 4 Biosafety Level 4 Practices, safety equipment, and facility design and
construction are applicable for work with dangerous and exotic agents that have a
high individual risk of life-threatening disease, which may be transmitted via the
aerosol route and for which there is no available vaccine or therapy. Agents with a
close or identical antigenic relationship to Biosafety Level 4 agents also should be
handled at this level. When sufficient data are obtained, work with these agents may
continue at this level or at a lower level. Viruses such as Marburg or Congo-Crimean
hemorrhagic fever are manipulated at Biosafety Level 4.
The primary hazards to personnel working with Biosafety Level 4 agents are
respiratory exposure to infectious aerosols, mucous membrane or broken skin
exposure to infectious droplets, and autoinoculation. All manipulations of potentially
infectious diagnostic materials, isolates, and naturally or experimentally infected
animals pose a high risk of exposure and infection to laboratory personnel, the
community, and the environment.
The laboratory worker's complete isolation from aerosolized infectious materials is
accomplished primarily by working in a Class III BSC or in a full-body, air-supplied,
positive-pressure personnel suit. The Biosafety Level 4 facility itself is generally a
separate building or completely isolated zone with complex, specialized ventilation
requirements and waste management systems to prevent release of viable agents to
the environment.
A dedicated non-recirculating ventilation system is provided. Only laboratories with
the same HVAC requirements (i.e., other BSL-4 labs, ABSL-4, BSL-3-Ag labs) may
share ventilation systems if gas-tight dampers and HEPA filters isolate each
individual laboratory system.
The supply and exhaust components of the ventilation system must be designed to
maintain the laboratory at negative pressure to surrounding areas and provide
differential pressure or directional airflow, as appropriate, between adjacent areas
within the laboratory.
Redundant supply fans are recommended. Redundant exhaust fans are required.
Supply and exhaust fans must be interlocked to prevent positive pressurization of the
laboratory.
The ventilation system must be monitored and alarmed to indicate malfunction or
deviation from design parameters. A visual monitoring device must be installed near
the clean change room so proper differential pressures within the laboratory may be
verified prior to entry.
NSF essentially repeats the BSL definitions
from the BMBL.