Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 64
Topic Requirement(s) Commentary
Ventilation
for Biosafety
Level 2
ASHRAE, 2011 Handbook - HVAC Applications, Laboratories, Pg. 14.16,
Containment Laboratories:
Biosafety Level 2
Federal guidelines for these laboratories contain minimum facility
guidelines….however typical HVAC design criteria can include the following:
• 100% outdoor air systems
• 6 to 15 air changes per hour
• Directional airflow into the laboratory rooms
• Site-specified hood face velocity at fume hoods (many institutions specify
80 to 100 fpm)
• An assessment of research equipment heat load in a room.
• Inclusion of biological safety cabinets
U.S. Dept. of Health and Human Services, Centers for Disease Control and
Prevention publication: Biosafety in Microbiology and Biomedical Laboratories,
(BMBL) 5th edition 2009:
There are no specific requirements for ventilation systems. However, planning of new
facilities should consider mechanical ventilation systems that provide an inward flow
of air without recirculation to spaces outside of the laboratory.
BSCs must be installed so that fluctuations of the room air supply and exhaust do not
interfere with proper operations. BSCs should be located away from doors, windows
that can be opened, heavily traveled laboratory areas, and other possible airflow
disruptions
BSCs can also be connected to the laboratory exhaust system by either a thimble
(canopy) connection or directly exhausted to the outside through a hard connection.
Provisions to assure proper safety cabinet performance and air system operation
must be verified.
HEPA filtered exhaust air from a Class II BSC can be safely recirculation back into
the laboratory environment if the cabinet is tested and certified at least annually and
operated according to manufacturer’s recommendations.
American Institute of Architects, GUIDELINES FOR PLANNING AND DESIGN OF
BIOMEDICAL RESEARCH LABORATORY FACILITIES 1999
C.11.3 BL-2 b. Directional Airflow: All laboratories shall have single-pass air. The
airflow shall be directed from clean areas to potentially contaminated areas and from
low-hazard to high-
hazard area. Air supply diffusers must be supplied with diffusers to
direct air away from fume hoods and BSCs in order to minimize potential disruptive
air currents.
Ventilation systems serving laboratories with a
BL2 or higher classification should be
designed to allow easy access for regular
inspection, testing and adjustment without the
necessity to disturb or upset the laboratory
function. Locating ventilation components
(terminal units, filters, distribution ducts, etc.)
in an interstitial space is recommended.
The AIA requires directional air flow. However,
the CDC only suggests it.