Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 66
Topic Requirement(s) Commentary
Ventilation
for
Biosafety
Level 3
(Continued)
American Institute of Architects, GUIDELINES FOR PLANNING AND DESIGN OF
BIOMEDICAL RESEARCH LABORATORY FACILITIES 1999
C.11.4 BL-3 b. Containment Requirements: Laboratories require all of the design
considerations for BL-2 laboratories plus specific requirements for additional
containment of those bio-hazardous materials used in the laboratory.
Ventilation must be single-pass air, and all BL-3 space must be kept negative with
respect to outside corridors and laboratories. Exhaust ducts must be under negative
pressure until the air is discharged outside the building.
Supply and exhaust ducts for BL-3 laboratories must be supplied with gas-tight
dampers to maintain the capability of gas decontamination of the laboratory without
compromising the rest of the building. Ductwork between the laboratory and the
damper must be gas tight.
n. Alarms: BL-3 facilities must be designed to ensure notification of inappropriate
directional airflows. Both visual (gauges) and audible local alarms are acceptable. In
addition, alarms indicating the potential failure of BL-3 containment shall be tied to a
central system at the Building Engineer’s office, where possible. Notification devices
shall indicate the failure to maintain a negative pressure differential between a non-
contaminated area and potentially contaminated areas.
All alarm systems shall be validated prior to occupancy of the containment space by
research personnel.
o. Filtration of Laboratory Exhaust: The need for HEPA filtration shall be
determined on a case by case basis in consultation with the facility safety personnel
and shall be based on a hazard assessment of the materials in use and the
procedures to be performed.
p. Autoclave Exhaust Filtration: The exhaust from an autoclave contains a
significant amount of moisture, and exhaust ductwork shall be designed accordingly.
Filtration of this exhaust, when necessary, (as determined above in Filtration of
Laboratory Exhaust) must be through a moisture-resistant (hydrophobic) filter such as
a 0.2 micron filter or equivalent. Filtration of moist exhaust through a cold filter
housing containing a paper HEPA filter will result in the destruction of the HEPA filter
and a break in integrity.
q. HEPA Filter Housings: When installed, HEPA filter exhaust housings must be
constructed in such a manner as allow for appropriate particulate testing (that is, DOP
or equivalent) and must be capable of being isolated from the ventilation system for
gas decontamination and testing (that is, gas-tight dampers and housings). Facility
safety personnel must be consulted with regard to the suitability of the
decontamination mechanism design and approve the system prior to finalization of
the design.
While BMBL requires sealable penetrations”
AIA specifies gas-tight dampers and ducts.