Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Biological Laboratories
Siemens Industry, Inc. 71
Topic Requirement(s) Commentary
Containment
Levels and
Ventilation
Requirements:
Canada
Public Health Agency of Canada, Office of Laboratory Security, Biosafety
Division, Laboratory Biosafety Guidelines, 3
rd
Edition 2011:
Containment level 1 (CL1)
No special ventilation features are required.
Containment level 2 (CL2)
1. 100% Outside air is recommended for room ventilation
Containment Level 3 (CL3)
1. 100% Outside air for room ventilation
2. Directional inward airflow provided such that air will always flow towards areas
of higher containment (e.g., ± 25 Pa differential).
3. Visual pressure differential monitoring devices to be provided at entry to
containment laboratory.
5. Alarm (visual or audible) to be provided in the laboratory and outside laboratory
area (that is, to warn others and maintenance personnel) to signal air handling
systems failure.
6. Where determined necessary by a local risk assessment, supply air duct to be
provided with backdraft protection (that is, HEPA filter; bubble tight backdraft
damper).
8. Supply air system to be independent of other laboratory areas. CL3 supply can
be combined with areas of lower containment when provided with backdraft
protection (that is, HEPA filter, bubble tight backdraft damper) downstream
from the connection. (For CL3 laboratories manipulating organisms, such as
HIV, that are not infectious via inhalation this criterion is only recommended.)
9. Supply air system to be interlocked (that is, fans, dampers, electrical) with
exhaust air system, to prevent sustained laboratory positive pressurization.
10. Exhaust air to be HEPA filtered. (CL3 laboratories manipulating organisms,
such as HIV, that are not infectious via inhalation are not required to fulfill this
criterion.)
12. HEPA filters installed in the supply and exhaust system to conform to the
requirements of: HEPA and ULPA filters. IEST-RP-CC001.3. Rolling Meadows,
IL: The Institute of Environmental Science and Technology, 1993.
14. Where HEPA filters are used for backdraft protection in accordance with local
risk assessment, supply HEPA filter housings to be designed to withstand
structural change at applied pressure of 2500 Pa [10 in. w.g.].
15. Exhaust HEPA filter housings to be designed to withstand structural change at
applied pressure of 2500 Pa [10 in. w.g.] and to be provided with a method of
isolation and decontamination. For CL3 laboratories manipulating organisms,
such as HIV, that are not infectious via inhalation this criterion is only
recommended.
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