Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Biological Laboratories
Siemens Industry, Inc. 83
Topic Requirement(s) Commentary
Biological
Safety
Cabinets and
Classifications
(Continued)
Class II Type B1 cabinets
• Minimum of 100 FPM (0.5m/s) inflow.
• HEPA filtered largely uncontaminated recirculated air.
• Exhausts most contaminated air to atmosphere through a dedicated duct
system.
• Has negative pressure duct system or surrounded by a negative pressure
duct system.
Class II Type B2 cabinets
• Minimum of 100 FPM (0.5m/s) inflow.
• HEPA filtered non-recirculated, downward airflow.
• HEPA filtered exhaust air to atmosphere.
• Has negative pressure duct system or surrounded by a negative pressure
duct system.
8.4.3 Class III Cabinets
• Description Provides absolute personnel protection, environmental
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protection and may provide product protection. It is characterized by a totally
enclosed, gas tight. negative pressure, HEPA filtered, ventilated workspace
accessed through attached rubber gloves and purged interchange chambers.
Exhaust air is treated by double HEPA filtration and/or incineration.
American Conference of Governmental Industrial Hygienists (ACGIH)
INDUSTRIAL VENTILATION A Manual of Recommended Practice, 27th Edition
10.35.2 Biological Safety cabinets
A laboratory hood…could be considered a Class I BSC if the exhausted air is
passed through HEPA filters prior to release to the atmosphere.
Type A cabinets that discharge into the work area are not recommended for use
with gasses or vapors.
ASHRAE, 2011 Handbook - HVAC Applications, Laboratories, Pg. 14.6, - 14.8
BIOLOGICAL SAFETY CABINETS
• Class I Similar to chemical fume hood, no research material protection, 100%
exhaust through a HEPA filter
• Class II Type A1 70% recirculation within the cabinet; 30% exhaust through a
HEPA filter; common plenum configuration; can be recirculated into the
laboratory
• Type A2 70% recirculation within the cabinet; 30% exhaust through a HEPA
filter; common plenum configuration; can be recirculated to the room or
exhausted to the outside
(Continued on Next Page)
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Environmental protection requires a HEPA filtering in the exhaust duct prior to discharging outdoors.