Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 86
Term Definition Commentary
Biological
Safety
Cabinets and
Classifications
(Continued)
Class II Type B1 and Type B2 cabinets rely on the building exhaust system to pull
the air from the cabinet’s workspace and through the exhaust HEPA filters.
Because containment in this type of cabinet depends on the building’s exhaust
system, the exhaust fan(s) should have redundant backups.
Class III Cabinets
The Class III cabinet is a gastight, negative pressure containment system that
physically separates the agent from the worker. These cabinets provide the highest
degree of personnel protection. Work is performed through arm-length rubber
gloves attached to a sealed front panel. Room air is drawn into the cabinet through
HEPA filters. The American glovebox Society (AGS 2007) indicates that Class III
cabinets should be maintained at 0.5 in. of water below ambient pressure. Exhaust
flow rate should provide a minimum of 100 fpm inward containment velocity through
a glove port opening in the event of a glove being inadvertently removed. HEPA
filtration or incineration before discharge to the atmosphere removes or destroys
particulate material entrained in the exhaust air.
Class III systems can contain highly infectious materials and radioactive
contaminants. Although there are operational inconveniences with these cabinets,
they are the equipment of choice when a high degree of personnel protection is
required. Note that explosions have occurred in Class III cabinets used for research
involving volatile substances
Biosafety
Cabinet
Applications
(See Types of Biological Laboratories & Required Type of Biosafety Cabinet – A
Summation of Applicable Code & Standards Requirements on the following page)
The following table provides a
comprehensive listing of biological
laboratory levels, anticipated hazard, and
suitable biological safety cabinets along with
the appropriate biosafety cabinet exhaust
arrangements. This table is a summation of
the descriptions and requirements of all
standards that cover biological laboratories
and biosafety containment devices.