Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 88
Topic Requirement(s) Commentary
Biosafety Cabinets
– Installation and
Safe Usage
Recommendations
National Sanitation Foundation, NSF 49 -2008, Biosafety Cabinetry:
Design, Construction, Performance and Field Certification
E.1 Location
E.1.1 The Class II (laminar flow) biosafety cabinet should be located out of the
traffic pattern and away from room air currents that could disrupt the
containment provided by the work access opening air barrier.
E.1.2 If there is a window in the laboratory, it should remain closed at all times.
Cabinets should not be located where room ventilation air inlets blow across
the front opening or onto the exhaust filter.
E.1.3 Where space permits, a 12 in (30 cm) clearance should be provided
behind and on each side of the cabinet. If not feasible, a minimum 3 in (8 cm)
clearance on each side and 1.5 in (3.8 cm) clearance in back are
recommended. The electrical outlet for the cabinet should be accessible for
the cabinet service and electrical safety testing without moving the cabinet.
E.2 Recommendations for installation
E.2.1 Type A1 and A2 cabinets Type A1 and A2 cabinets are designed to
return air to the laboratory and do not generally require external venting. It is
critical that a minimum of 3 in (8 cm) clearance be provided between the
exhaust opening on top of the cabinet and the ceiling. Less than 3 in (8 cm)
clearance constricts the exhaust and reduces the flow into the cabinet at the
front access opening. At least 12 in (30 cm) clearance is required between the
exhaust opening on top of the cabinet and the ceiling to allow the use of a
thermal anemometer to measure the exhaust velocity when calculating the
cabinet inflow velocity. When it is desirable to exhaust air to the atmosphere,
exhaust should be via a 100% exhaust system (i.e., a system that does not
recirculate its exhaust air into other parts of the building). The recommended
exhaust system connection for types A1 and A2 cabinets is an exhaust canopy
connection. Every canopy design must be tested to determine the airflow rate
exhausted by the canopy that will ensure performance. Whenever the cabinet
is field certified, the minimum exhaust flow by the canopy should be verified by
measurements using the approved instruments and techniques. No type A
cabinet should ever be hard connected to an exhaust system.
It is preferable that cabinets be installed using an exhaust connection that
allows for scan testing of the exhaust HEPA filter. A properly designed and
installed exhaust canopy will allow a Type A1 or A2 cabinet to maintain
acceptable inflow velocity at the front access opening even when the flow
through the exhaust canopy is completely stopped. The performance of the
exhaust canopy should be assessed by either the manufacturer of the exhaust
canopy or the user to ensure awareness of the performance characteristics of
the exhaust canopy with the particular model of cabinet being exhausted.
When the exhaust canopy is used to capture hazardous nonparticulate
material being exhausted from the cabinet, the exhaust and associated alarm
system should meet the same criteria as indicated for the Types B1 and B2
cabinets.