Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Biological Laboratories
Siemens Industry, Inc. 89
Topic Requirement(s) Commentary
Biosafety
Cabinets –
Installation and
Safe Usage
Recommendations
(Continued)
When Type A1 and A2 cabinets are found to be directly attached to the
exhaust system and vented to the outside without the use of an exhaust
canopy, it is recommended that the exhaust connection be modified to an
exhaust canopy.
E.2.2 Types B1 and B2 cabinets
Type B1 and B2 cabinets are to be vented outside the building without
recirculation. The venting system should include a leak-tight duct, a damper in
the duct near the cabinet to permit flow adjustment closure and
decontamination, and an external exhaust fan as the final system component.
The exhaust fan should be sized to deliver the required exhaust airflow (as
specified by the cabinet manufacturer), considering pressure losses in the duct
and allowing at least 2 in w.g. (500 Pa) for a dirty HEPA filter. If a charcoal
filter is used downstream of the HEPA filter, an additional pressure capacity
equal to the manufacturer's recommended resistance should be provided. An
alarm should be provided at the cabinet to indicate loss of exhaust flow. This
can be an exhaust volume flow measuring device in the duct downstream of
the exhaust filter, a sail switch at the fan discharge, or a flow measuring station
in the exhaust duct. It is recommended that each Type B1 or B2 cabinet have
its own (dedicated) exhaust system. The cabinet should be interlocked with the
blower in the duct or the building system to prevent pressurization of the
exhaust system. In addition, cabinets hard connected to an exhaust system It
is recognized that there is interest in utilizing the increasingly sophisticated
modulated flow exhaust ventilation systems where the exhaust from Type B1
or B2 cabinets, chemical fume hoods, flexible exhaust hoses, and/or room
exhausts are modulated based on use to optimize containment, maintain
appropriate pressure differentials, and maximize energy savings by reducing
overall exhaust volume. These systems are required to maintain a high level of
control of many complex factors over a number of years. Although the
potential cost savings are great, the severity of the hazards contained by the
biological safety cabinets requires the use of simpler and more reliable
constant flow systems for the cabinet exhaust. If a modulated flow exhaust
system is used, it is recommended that the operation of the cabinet exhaust be
verified under a variety of conditions over time. Furthermore, the type of
exhaust alarm must be assessed in light of the type of sensors and controls
used in the modulated flow system.
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