LABS (Laboratory Archival Biological Storage) CRYOSTORAGE SYSTEM OPERATION & MAINTENANCE INSTRUCTIONS CAUTION - SAFETY FIRST! • REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN THE “HANDLE WITH CARE” BOOKLET FORM # TW-10 P/N 7950-8052 BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM. • DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND THESE INSTRUCTIONS. • DO NOT PERMIT UNTRAINED PERSONS TO USE OR MAINTAIN THIS UNIT.
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LABS TABLE OF CONTENTS SAFETY PRECAUTIONS FOR LIQUID NITROGEN............................................ 3 Extreme Cold - Cover Eyes and Exposed Skin ......................................................... 3 Keep Equipment Area Well Ventilated .................................................................... 3 Liquid Nitrogen System ........................................................................................... 3 Dispose of Waste Liquid Nitrogen Safely ....................................
LABS Figure 1.0 A master computer program works with the KRYOS software system to control and monitor this bank of LABS cryostorage units. Figure 2.0 & 2.1 Retractable steps make retrieval of stored samples easy and efficient. TW-LABS-Manual-Digital-TOC-BlackandWhite.
LABS SAFETY PRECAUTIONS FOR LIQUID NITROGEN Nitrogen is an inert, colorless, odorless, and tasteless gas making up four-fifths of the air you breathe – and can be hazardous. Air is roughly one-fifth oxygen. Liquid nitrogen is at a temperature of -196°C (-320°F) under normal atmospheric pressure. Extreme Cold - Cover Eyes and Exposed Skin Accidental contact of liquid nitrogen or cold issuing gas with the skin or eyes may cause a freezing injury similar to frostbite.
LABS GENERAL INFORMATION This state-of-the-art Cryogenic System can be used in either the liquid or vapor phase. Units are supplied with Taylor‑Wharton approved controllers. If other liquid level controllers are used, please contact Taylor-Wharton before putting the refrigerator into service. Taylor-Wharton LABS Cryostorage Systems are designed for applications where extremely low temperature storage of biological products is required.
LABS LABS Cryostorage Specifications Liquid nitrogen at atmospheric pressure weighs 1.78 lb./liter (0.8 kg/liter). All Taylor-Wharton Gas Equipment CryoScience systems are designed to support the full weight of liquid nitrogen and a complete stainless steel or aluminum inventory control system with boxes and specimens. Dimensions 20K 38K 40K 80K 94K External Operating Height (Top of Step to Lid opening) in. mm 42 1067 44 1118 42 1067 42 1067 40 1016 Step Height1 in. mm 11 279 11.
LABS INSTALLATION Unpacking and Inspection Inspect shipping containers for external damage. All claims for damage (apparent or concealed) or partial loss of shipment must be made in writing within five (5) days from receipt of goods. If damage or loss is apparent, please notify the appropriate parties as indicated below: Domestic LTL Shipments – The customer should notify and file the appropriate damage claims with the carrier. All products are shipped FOB Origin. Figure 4.
LABS A. System model and serial number, and controller model and unit, if available. B. User’s name, company, address, and phone number C. Malfunction symptoms If possible, the original packing material should be retained for reshipment. If not available, consult Taylor-Wharton for shipping and packing instructions. It is the responsibility of the customer to assure that the goods are adequately packaged for return to the factory.
LABS WARNING: If the fill fails to stop for any reason, quickly close the liquid supply valve to prevent overfilling until the cause of the problem can be determined. Initial Fill The LABS Cryostorage System comes preset for vapor phase storage from the factory. The liquid nitrogen supply pressure at the inlet to the refrigerator should be in the range of 10 psig (0.7 bar/69 kPa) to 22 psig (1.4 bar/152 kPa) for optimum performance.
LABS Inventory Control System Layouts (LABS Cryostorage System) Figure 5.0 Vial Storage in Boxes on Racks • 100 Vial Polycarbonate Typical Rack with Box Design • 100 Vial Aluminum Boxes • 81 Vial Polycarbonate, • Cardboard, or • Stainless Steel Boxes LABS 20K LABS 38K LABS 40K LABS 80K LABS 94K NOTE: Racks are not square. Orientation is important.
LABS Figure 6.0 Rack Arrangements for 50 ml Cassettes Typical Cassette Design LABS 20K LABS 38K LABS 40K LABS 80K LABS 94K Part Number Capacity Frames Cassettes LABS 20K 924 132 924 LABS 38K 1582 226 1582 LABS 40K 1876 268 1876 LABS 80K 3381 483 3381 LABS 94K* 3864 483 3864 Figure 6.0 Rack Arrangements for 50 ml Cassettes *LABS 94K use an 8-level rack. All other units utilize a 7-level rack configuration. 10 TW-LABS-Manual-Digital-TOC-BlackandWhite.
LABS Figure 7.0 Rack Arrangements for 250 ml Cassettes Typical Cassette Design LABS 20K LABS 38K LABS 40K LABS 80K LABS 94K Part Number Capacity Frames Cassettes LABS 20K 500 100 500 LABS 38K 900 180 900 LABS 40K 990 198 990 LABS 80K 1920 384 1920 LABS 94K* 2304 384 2304 Figure 7.0 Rack Arrangements for 250 ml Cassettes *LABS 94K use a 6-level rack. All other units utilize a 5-level rack configuration. 11 TW-LABS-Manual-Digital-TOC-BlackandWhite.
LABS Figure 8.0 Rack Arrangement for 500 ml Cassettes Typical Cassette Design LABS 20K LABS 38K LABS 40K LABS 80K LABS 94K Part Number Capacity Frames Cassettes LABS 20K 420 84 420 LABS 38K 690 138 690 LABS 40K 830 166 830 LABS 80K 1610 322 1610 LABS 94K* 1932 322 1932 Figure 8.0 Rack Arrangement for 500 ml Cassettes *LABS 94K use a 6-level rack. All other units utilize a 5-level rack configuration. 12 TW-LABS-Manual-Digital-TOC-BlackandWhite.
LABS MAINTENANCE Strainer Cleaning Instructions The container will not fill properly if the strainer is clogged with ice or dirt. To clean the strainer, first close the supply valve to the refrigerator. Vent the fill line of all pressure. Remove and warm the strainer to ambient temperature. Purge the strainer from both directions with dry nitrogen gas or dry oil-free air. Rinse the strainer with alcohol and purge it again with dry nitrogen gas or dry oil-free air to clear contaminants.
LABS To clean and sterilize your defrosted LABS Cryostorage System, spray the entire inner vessel surface with ample amounts of an approved & compatible disinfectant. Allow surface contact to be maintained for a minimum of five minutes. Rinse the inner vessel with water, remove all water and debris through the access opening located in the carousels bottom tray, and vacuum or towel dry the surface.
LABS FREEZER SERVICE AND MAINTENANCE HISTORY LOG Fill in top section at installation. Copy this form each time service is required. Fill bottom section with service notes to keep a complete log of each freezer service and maintenance history.
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4075 Hamilton Boulevard, Theodore AL 36582 TWSalesandService@taylorwharton.com www.taylorwharton.com Europe +421 (0)55-727-7157 07/2012 TW-LABS-Manual-Digital-TOC-BlackandWhite.indd 18 CryoScience Information Centers: Americas +251-443-8680 • In U.S. and Canada: 800-898-2657 ©2012 Taylor-Wharton International. All Rights Reserved.
