HB-1D Hybridisation oven OPERATOR’S MANUAL Issue 26 07/2014
CONTENTS INTRODUCTION ........................................................................................................ 5 BEFORE USE............................................................................................................. 6 Unpacking.................................................................................................................................................6 SAFETY AND INSTALLATION ................................................................................
Support contacts .................................................................................................................................... 29 SPARES AND ACCESSORIES ................................................................................ 30 DECLARATION OF CONFORMITY .........................................................................
INTRODUCTION Please read all the information in this manual before using the unit. The Techne HB-1D hybridisation oven is ideal for blotting techniques in which RNA, DNA or protein are immobilised onto nylon or nitrocellulose filters. Several filters can be hybridised simultaneously in a single tube while the rotating design ensures volumes as low as 5ml can be used and recovered. The HB-1D has unique “slot-in” tubes with adjustable tube rotation speeds of 0 to 20 rpm; controllable to suit your application.
BEFORE USE Before using the HB-1D please make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you.
SAFETY AND INSTALLATION Please read all the information in this manual before using the HB-1D. WARNING HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite combustible material.
I Mains Switch On O Mains Switch Off Replacement cable 2 Should the mains lead need replacement, a cable of 1mm of harmonized code H05VV-F connected to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
SÉCURITÉ ET CONSIGNES D’INSTALLATION Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le HB-1D. AVERTISSEMENT Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l’opérateur et enflammer les matériaux combustibles.
3. Placer l’appareil sur une surface plane ou si nécessaire sous une hotte d’aspiration, veiller à ce que les trous d’aération situés sous l’appareil ne soient pas obstrués. 4. Raccorder le câble d’alimentation à la prise située à l’arrière de l’appareil. 5. Allumer l'appareil.
SICHERHEITS- UND INSTALLATIONSINFORMATIONEN Lesen Sie diese Anleitung vor Verwendung des HB-1D bitte sorgfältig durch. ACHTUNG HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Techne hat bei der Konstruktion dieses Gerätes sehr darauf geachtet, daß der Bediener vor Gefahren geschützt ist.
I Netzschalter Ein O Netzschalter Aus Ersatzkabel 2 Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTROFACHMANN HINZUZIEHEN.
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE Leggere attentamente il presente manuale prima di usare il HB-1D. AVVERTENZA Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile.
3. Collocare l'unità su un banco piano idoneo o in una cappa aspirante se necessario, assicurandosi che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti. 4. Inserire il cavo di rete nella presa che si trova sul retro dello strumento. 5. Accendere lo strumento: I Interruttore di rete Acceso O Interruttore di rete Spento Cavo di ricambio 2 Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm codice armonizzato H05VV-F collegato ad una spina IEC 320.
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN Lea atentamente este manual antes de utilizar el HB-1D. ADVERTENCIA LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible.
3. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción. 4. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento. 5.
CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne dealer or: UK Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA UK Tel: +44 (0)1785 812121 Fax: +44 (0)1785 810405 E-mail: sales@bibby-scientific.com www.techne.com North and South America Bibby Scientific US Inc. t/a Techne Inc. 3 Terri Lane, Suite 10 Burlington, N.J. 08016 USA Toll Free (in NA): 800-225-9243 Tel: +1 609 589 2560 Fax: +1 609 589 2571 E-mail: labproducts@techneusa.com www.techneusa.
SPECIFICATIONS Tube capacity Maximum temperature* 6 (large and small in any combination) 24 mini tubes (when using the FHB61 mini tube holder) 96 x 15ml tubes (in holder) 48 x 50ml tubes (in holder) 100°C Minimum temperature 10°C above ambient Temperature selection Digital Adjustable rotation speed Adjustable rocking platform (optional) 0; 5 to 20 rpm 30 opm recommended Temperature display LED Temperature set point resolution 0.
INSTALLATION See also the sections on Safety and Installation. 1. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet and outlet vents are free from obstruction. 2. Place some water in a hybridisation tube and fit the tube in the HB-1D. Adjust the feet on the base of the unit by unscrewing them anticlockwise slightly until the unit is level. Check the level by placing the tube in different positions in the oven. 3.
Front view of the HB-1D 20
OPERATION SWITCHING ON Once your HB-1D is connected to a power supply, switch it on using the power switch next to the power inlet socket on the rear panel. THE FRONT PANEL CONTROLS The front panel controls consist of a four digit LED temperature display, three buttons for controlling the display, three indicators and a speed control knob for adjusting the tube rotation speed. The front panel controls When you first switch on, the display will show the edition of the software installed on the unit.
SETTING THE TEMPERATURE 1. To display the set temperature, press the SET temperature button: 2. To increase the set temperature, hold down the SET temperature button and press the Increase button. 3. To decrease the set temperature, hold down the SET temperature button and press the Decrease button. Each press of the Increase or Decrease buttons will increase or decrease the set temperature by 0.1°C. If the buttons are held down the temperature change will accelerate to 5°C per second.
INDICATORS 1. Power indicator. When lit, this shows that there is power to the unit. 2. Heater indicator. When this is lit or flashing, it indicates that the heater is heating the unit or controlling the temperature. If the oven is above the set temperature then the indicator will be off. 3. Over-temperature indicator. If this is lit, it indicates that the unit has developed a fault and the over-temperature cut-out has been triggered.
HYBRIDISATION TUBES The hybridisation tubes are manufactured from durable thick borosilicate glass with a non-drip design. The tubes must be checked regularly and replaced if any damage occurs. Any damage to the ends of the tubes can cause them to break or leak when the end caps are inserted. Large (80mm diameter) tube assembly (FHB12) Remove the tube end cap which has the two holes in it. Place the filters in the tube and replace the end cap.
Mini (4 x 32mm) tube assembly (FHB41 and FHB43) The mini tubes must be used in the FHB61 multi-tube holder. Unscrew the end cap of the tube. Place the filters and the liquids appropriate to the protocol in the tube and replace the end cap. Fix the tube into a multi-tube holder, using the springs, and ensure that there are four tubes in the holder. The end caps must be as shown, on alternate ends. The multi-tube holder will not rotate properly if there are not four mini tubes in it.
INSTALLING THE HYBRIDISATION TUBES 1. Pre-heat the oven to the required temperature. 2. Insert the tubes or tube holder into the HB-1D ensuring that they rotate freely and the end caps engage on the drive rollers on the right had side of the chamber. 3. Close the door. 4. Turn the speed control knob until you reach the required speed.
DO NOT TIP THE TUBE SO THAT THE COLOURED END IS DOWN AS THIS WILL CAUSE A SPILLAGE. 3. The unit has been designed to conform to international safety specifications and is fitted with a resettable over-temperature cut-out. If, for any reason, the temperature rises above a safe level for the instrument, the over temperature protection cuts out the heater, but the tubes continue to rotate. Check the reason for the cut-out; if there is no obvious cause, reset the cut-out once the unit has cooled.
ADDITIONAL INFORMATION Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment. FAULT FINDING Should you have any problems with your HB-1D which cannot be easily remedied, you should contact your supplier and return the unit if necessary. Contact the Bibby Scientific Ltd.
SUPPORT CONTACTS If you require further technical or application assistance please contact Techne at: E-mail: technehelp@bibby-scientific.com. Phone: +44 (0)1785 810433 For servicing information please contact: Bibby Scientific Ltd. Service Department Beacon Road Stone Staffordshire ST15 0SA E-mail: service@bibby-scientific.com. Phone: +44 (0)1785 810475 Please note that neither Bibby Scientific Ltd. nor any of their agents can accept the return of ANY GLASSWARE from these units.
SPARES AND ACCESSORIES The following spares and accessories may be purchased from Bibby Scientific Ltd. or their dealers. All Techne tubes are made from durable Borosilicate glass. Contact Bibby Scientific Ltd. or your Techne dealer for further details. Part code Description FHB12 Large (80mm diameter) hybridisation tube assembly complete with sealing rings and end caps.
DECLARATION OF CONFORMITY 31
