P H A R M A C I A B I O T E C MultiTemp III ® User Manual 18-1106-33 Edition AD A H
Important user information Reading this entire manual is necessary for full understanding and safe use of this product. Should you have any comments on this manual, we will be pleased to receive them at: Pharmacia Biotech AB S-75182 Uppsala Sweden Pharmacia Biotech reserves the right to make changes in the specifications without prior notice. Warranty and Liability Pharmacia Biotech AB guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications.
Contents 1. Introduction..................................................................... 3 2. Safety information ........................................................... 3 2.1 Safety precautions................................................. 3 2.2 Safety standards.................................................... 4 2.3 Safety features ...................................................... 5 3. Installation....................................................................... 6 3.
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1. Introduction and 2. Safety information 1. Introduction MultiTemp® III circulates constant temperature liquid to external electrophoresis equipment. The liquid temperature range is -10 to +90 °C. The unit contains KLEA 134a*, an ozone friendly HFC-type coolant. MultiTemp III both supplies coolant for high voltage techniques and provides temperature control at higher temperature (e.g. 60 °C) when performing DNA sequencing.
1. Introduction and 2. Safety information 7. Do not use the circulator beyond its specifications or for other than its intended use. 8. Do not touch the stainless steel bath cover and tubings when the unit is used at a high working temperature. Under these conditions, the surface temperature can rise up to approximately 70 °C. 2.
1. Introduction and 2. Safety information 2.3 Safety features General cut-off The heater, the compressor and the circulation pump can be switched off by a relay built into the unit. This general cutoff will be activated if a malfunction is detected by the safety sensors. See 7.4, Description of Safety Features. Protection against over-heating A built-in temperature limit switch protects against uncontrolled heating of the bath liquid by activating the general cut-off if this limit is exceeded.
3. Installation 3. Installation 3.1 Unpacking and installation 1. Check the contents against the packing list supplied. Inspect for any damage that may have occurred during transit. Report any damage immediately to your local Pharmacia Biotech representative and to the transport company concerned. 2. Place MultiTemp III in its working location, ensuring adequate space for ventilation of air through the grids on the sides (air outlet) and rear (air inlet) of the unit. 3.
3. Installation 3.2. Tubing connections Silicone tubing (inner diameter 8 mm) can be used over the entire working temperature range of the unit. PVC tubing can only be used in the temperature range of 10 to 60 °C. 1. Connect the inlet and outlet tubing to the nozzles on the rear of the circulator (Fig. 1). 2. Connect the other ends to the electrophoresis unit. The nozzles have an outer diameter of 9.5 mm. To ensure safe tubing connections, secure all tubing with hose clamps.
3. Installation liquid pour into a large vessel using hydrostatic pressure. The bath contains 3 litres of liquid, plus the volume in tubing and connected equipment. The last millilitres can be removed by opening the screw on the front of the unit, (see Fig. 1). Warning! Do not drain hot water. Use protective gloves and clothing when draining the bath liquid. Compare the local mains voltage and frequency with the spe3.
4. Operation 4. Operation 4.1 Start-up 9 8 10 1 3 2 7 4 6 5 Fig. 2. Front panel of MultiTemp III. 1. 2. 3. 4. 5. Mode selector Set value down Set value up Enter Reset 6. Mains switch 7. Control lamp cooling on/off 8. Display 9. Heater control sign 10. Display Before switching on, confirm that the outlet and inlet nozzles are either firmly connected to external equipment or to each other and that the tubing is secured with hose clamps. Switch the power on at the mains switch.
4. Operation the external system and the level in the bath will fall. Add more liquid until the level is once again 2 cm below the top plate. If the safety circuit is triggered, please top up with more liquid and restart the unit using . Repeat this action if necessary. Note: Check the liquid level after the unit has been in use for a while. When MultiTemp III is switched on, the compressor starts and the heater will be activated if the set temperature is greater then the actual temperature.
4. Operation 2. Use or to set the maximum allowed set temperature. The factory pre-set value is 90.0 °C. 3. Confirm with before the display returns to the actual temperature value. Note: The new value is not saved until sed. 1. Press 4.4 Temperature setting 2. Use has been pres- to select the S mode. or to set the working temperature. 3. Confirm with before the display automatically returns to the actual temperature value. Note: The new set value is not saved until pressed. 4.
4. Operation 5. Confirm with temperature value. before the display returns to the actual Note: The new value is not saved until sed.
5. Trouble shooting 5. Trouble shooting 5.1 Error messages Message on display Cause Remedy The bath temperature has exceeded Pour in more liquid. the safety temperature limit. Too low water level. Pour in more liquid. The winding of the pump motor is too hot. Check pump and wait a few minutes until the motor temperature has dropped. If this fault occurs often, return the unit for service. The temperature sensor is broken. The sensor must be exchanged by service personnel.
6. Maintenance 6. Maintenance 6.1 General Warning! Always disconnect MultiTemp III from the mains (Pull out the mains plug) before maintenance and/or service. Service should be carried out by authorised personnel only. To maintain efficient operation of the compressor cooling unit, have the air grid and liquifier (heat exchanger) checked and cleaned by service personnel two to four times a year, depending on the amount of dust in the air. Always ensure the liquid bath has sufficient circulating liquid.
6. Maintenance 6.2 Fuses The circuit breaker (thermal fuses) for MultiTemp III are located on the rear of the unit. Note: If the circuit breaker has been triggered, there may be some delay before it can be released. Please push the circuit breaker button only; do not pull.
7. Detailed Description 7. Detailed Description 7.1 General operating principle MultiTemp III regulates temperature by balancing the effects of constant cooling and controlled pulses of heating. Constant cooling is provided by a built-in unit that includes a compressor, an air cooled liquifier and an evaporator (a cooling coil as heat exchanger) located in the chamber. This cooling unit operates at temperatures up to 68.0 °C. Beyond this temperature, the compressor automatically switches off.
7. Detailed Description Mains Switch. Starts the cooling compressor and the pump motor that circulates the temperature controlled liquid. Activates the heater if the temperature set is greater than the actual temperature. Change the temperature value. Values are changed in an accelerating manner when either key is held down. Changes between showing the actual temperature and the various program modes. Actual temperature mode; shows the actual temperature in the bath. S mode; sets the working temperature.
7. Detailed Description 7.4 Description General cut-off The heater, the compressor and the circulation pump can be of safety switched off by a relay built into the unit. This general cutfeatures off will be activated if a malfunction is detected by the safety sensors. When the general cut-off switch is activated, the circulator can only be restarted after the cause of the indicated fault has been eliminated. The display will show when this has occurred.
7. Detailed Description Accurate Setting of Temperature Limit If a more accurate temperature limit is required, for example 50 °C, follow the procedure below. 1. 2. Set the temperature limit switch to its highest value. Set the working temperature to 50 °C according to Section 4.4. 3. Run the unit and wait until it reaches exactly 50 °C. 4. Turn the temperature limit dial very slowly anticlockwise (temperature down) until the safety circuit is triggered and the display indicates a malfunction. 5.
7. Detailed Description Note: Although this feature activates the general cut-off, it is not a dedicated ”safety-function” of the type listed above. When the error message shown above is displayed, the sensor must be exchanged by service personnel. Restart after power failure 7.5 Manual restart in operation mode 8 MultiTemp III automatically restarts after a power failure. Make sure that this automatic restart will not cause a dangerous situation to arise.
7. Detailed Description To start MultiTemp III in 8 mode ON, follow the instructions below: 1. Check that all tubings are firmly connected and secured with hose clamps. 2. Switch the power on with the mains switch. The display flashes . 3. Press for start. The version number of the software is briefly shown on the display. The pump motor and compressor start up, liquid starts to circulate, and the heater will be activated if the set temperature is greater than the actual temperature.
8. Technical specifiations 8. Technical specifications Technical specifications (ambient temperature 20 °C) Working temperature range -10 to 90 °C Temperature control constancy at 20 °C < ± 0.
9. Ordering information 9. Ordering information Designation Code No. MultiTemp III 100-120 V 18-1102-77 MultiTemp III 220-240 V 18-1102-78 Accessories Item Quantity Code No.
Short instructions Note: The following short instructions are intended as a guide to users who are fully familiar with safety procedures and operating instructions described earlier in this manual. 1. Check to make sure that all tubings are firmly connected and secured with hose clamps. 2. Check the liquid level and fill the bath if needed (the level should be 2-4 cm below the lid). 3. Switch the power on at the mains switch. 4. Set the safety temperature limit using the dial on the rear of the unit.
