MultiDoc-It™ Imaging System Installation and User Instructions UVP, LLC Ultra-Violet Products Ltd. 2066 W. 11th Street Upland, CA 91786 Phone: (800) 452-6788 / (909) 946-3197 Fax: (909) 946-3597 Unit 1, Trinity Hall Farm Estate Nuffield Road, Cambridge CB4 1TG UK Phone: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.
MultiDoc-It Imaging System 2 Introduction The MultiDoc-It Imaging System is a high resolution imager capable of capturing and documenting fluorescent gel images. The system combines: 1. 2. 3. 4. 5. MultiDoc-It Darkroom DigiCam digital color camera Ethidium Bromide (EtBr) Filter ® Doc-It LS Acquisition Software UV transilluminator (transilluminator instructions are provided in a separate manual) NOTE: MultiDoc-It TLC Systems (for Thin Layer Chromatography) do not include a transilluminator.
MultiDoc-It Imaging System 3 Doc-ItLS Software The Doc-ItLS software loads onto the user’s existing computer. The software controls the camera acquisition functions as well as enhancement and reporting features. For information on operation of the software, refer to the Doc-ItLS software help files. Doc-ItLS Acquisition Software UV Transilluminator A UV transilluminator may be shipped with the system. Refer to the Transilluminator manual for operating instructions.
MultiDoc-It Imaging System 4 System Setup DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO A POWER SUPPLY. CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment apparatus. Head Screw Power Connections 1.
MultiDoc-It Imaging System 5 CAUTION: All UVP Transilluminators are powerful sources of UV radiation that will cause damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected. UV Blocking Eyewear should be worn as well. Install Doc-ItLS Software NOTE: The following provides a brief instruction for installing the software. For additional instruction on using the software, refer to the Doc-ItLS help files. 1. 2. 3. 4.
MultiDoc-It Imaging System 6 System Operation Darkroom Operation 1. 2. 3. Turn on the green darkroom main power switch, located at the top of the MultiDoc-It darkroom. The main power switch controls the automatic transilluminator shut off switch and the white light power. To use the transilluminator, once the main power switch is on, turn on the green transilluminator power switch. If the darkroom door is opened, the transilluminator automatically shuts off to prevent accidental UV exposure.
MultiDoc-It Imaging System 3. 4. 5. 6. 7 To take images in color, ensure that the Color Capture box is checked. Select Preview at the top of the software to preview the image. While in preview mode, the Brightness slider can be adjusted to brighten dark images or vice versa. The user may adjust the focus manually to enhance the appearance of the previewed image.
MultiDoc-It Imaging System 2. 8 A new camera window appears with additional options: Camera Control in Manual Mode 3. 4. Select the desired options for an enhanced image. See the camera manual for detailed descriptions of options. Select Capture at the top of the software. Overhead UV Lamps (Purchased Separately) 1. Move the UV Lamp power switch to the ON position. 2. Slide the UV Lamps into the lamp brackets inside the darkroom.
MultiDoc-It Imaging System 9 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Replacement Parts and Accessories Accessories are available for use with the MultiDoc-It Imaging Systems. Contact UVP’s offices at the telephone numbers below for replacement parts for MultiDoc-It equipment. For transilluminator replacement parts, refer to the separate transilluminator manual.
MultiDoc-It Imaging System 10 3. The transilluminator itself has a power switch; make sure that the transilluminator power switch is in the “On” position. 4. Ensure that the transilluminator power jumper cord is securely connected at both the ends. Technical Assistance UVP offers expert technical support on all UVP products If there are any questions about product use, operation or repair, contact UVP’s offices at the locations below.