FirstLight UV Transilluminators Instruction Manual UVP, LLC 2066 W 11th Street, Upland, CA 91786 Tel: (800) 452-6788 / (909) 946-3197 Fax: (909) 946-3597 Ultra-Violet Products Ltd. Unit 1,Trinity Hall Farm Estate, Nuffield Road Cambridge CB4 1TG UK Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561 Web site: www.uvp.
FirstLight UV Transilluminators Page 2 Introduction UV transillumination is a ubiquitous tool in Life Science research. With few exceptions, fluorescent stains used in post electrophoresis analysis of proteins and nucleic acids have significant excitation peaks with ultraviolet (300-365 nm) light, making midrange UV the excitation source of choice for high sensitivity analysis for many fluorophores.
FirstLight UV Transilluminators Page 3 Specifications The FirstLight UV illuminator features a powder coat housing base and stainless steel frame. The stainless steel frame is coated with an antimicrobial substance that resists bacterial growth. The filter assembly is a unique combination of phosphored and filtered plates.
FirstLight UV Transilluminators Page 4 When the UV Blocking Cover is not being used, UV light may escape through the holes dedicated to accepting the bracket pins of the UV Blocking Cover. Remove the black safety plugs from their package Insert the safety plugs through the holes as shown. Safety Plug Set-Up Place the transilluminator on a level work surface. Be sure that an air space exists around the bottom of the work surface.
FirstLight UV Transilluminators Page 5 In the past, quantitative CCD imaging with UV has been difficult due to the lack of uniformity found in typical UV transilluminators. Accurate and repeatable UV imaging requires a uniform light source.
FirstLight UV Transilluminators Page 6 Servicing Procedures Servicing the Transilluminator The FirstLight UV illuminator has no serviceable parts at the customer location except the fuses. See below for replacement fuse part numbers. NOTE: The warranty is void if the unit is opened! The illuminator must be returned to the factory for repairs or replacement of parts, except the fuses. A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product.
FirstLight UV Transilluminators Page 7 Technical Support UVP offers technical support for all of its products. If you have any questions about the product’s use or, operation, please contact UVP’s offices at the following locations.
FirstLight UV Transilluminators Page 8 References 1. Waluszko, A.1, Cole, K.1, Osburn, M.2, Zhang, H.1, Galdamez, L.1, Meyer, R.1, Messenger, R.1, Chakravarti, B.3, Chakravarti D.3, Gallagher, S.R.1 , Applications of a Highly Uniform UV Transillumination Imaging System for Quantitative DNA and Protein Analysis, 2004 1UVP, LLC Upland, CA 91786; 2Scripps College, Claremont CA 91711; 3Keck Graduate Institute, Claremont CA 91711 2. Gallagher, S.R., B. Moomaw, and S.
