Operating instructions
Charged Aerosol Detectors
Corona Veo and Corona Veo RS
Operating Instructions Page 111
8.3.2 Maintaining the Detector between Analyses
Prevent build-up or accumulation of residue from mobile phase/analytes within the
detector between the analyses. To do so, periodically flush the detector. This helps
maintain consistent response, optimum performance and reduced detector downtime. In
general, make sure that you use the solvent with the best solubility characteristics for the
analyte(s) of the analytical method in question.
Remove the analytical column before you perform any of the following wash procedures.
Without column, a typical flow rate will be 2.0 mL/min.
Mobile phase and sample
composition
Recommended wash procedure
Reverse phase (small
molecules)
• A mixture of isopropyl alcohol (IPA) and HPLC-grade water. The
required amount depends on the gradient method used.
• High aqueous mixtures when using buffers over 100 Mm in strength.
Reverse phase (large molecules
and polymers)
100 % tetrahydrofuran (THF) or other appropriate organic solvent
as determined by the HPLC method used.
Reverse phase (proteins) A mixture of alcohol (IPA/methanol) and HPLC-grade water
(50:50).
Normal phase A mixture of tetrahydrofuran and methanol (50:50).
8.3.3 Cleaning the Surface of the Detector
Observe the following when cleaning the surface of the enclosure:
• Clean the surface of the detector with a soft towel moistened with a mild detergent. This
should be suitable for removing dust and fingerprints.
• Prevent any liquids from entering the inside the detector, as this could damage the
detector. Clean the display with a soft tissue.
If it becomes necessary to disinfect the enclosure surface, use a mild 10 % bleach solution.
However, do not allow the bleach solution to remain on the detector for more than a few
minutes. Wipe the solution off with a damp towel. After cleaning, dry the detector with a soft
towel.
8.3.4 Operational Qualification and Performance Qualification
Operational Qualification and Performance Qualification allow you to check and document
the performance of the HPLC system. All materials required for performing qualification and
detailed instructions are available on request.