Instrument User Manual 510731-10EN1 (06/2008) 24262 bioMérieux, Inc. Box 15969 Durham, North Carolina 27704-0969 / USA Tel. (1) 800-682-2666 EC [04] REP bioMérieux® SA au capital de 12 029 370 € 673 620 399 RCS LYON 69280 Marcy l’Etoile / France tél. 33 (0)4 78 87 20 00 / fax 33 (0)4 78 87 20 90 http://www.biomerieux.
Argentina bioMérieux Argentina Av. Congreso 1745 C1428BUE Capital Federal Buenos Aires tel. (54) 11 5555 6800 fax (54) 11 5555 6888 bioMérieux China Limited Room 2605, South Tower, World Trade Center 371-375 Huan Shi Dong East Road 510095 Guangzhou tel. (86) 20 8762 7010 fax (86) 20 8762 7015 Australia bioMérieux Australia P/L Unit 25 - Parkview Business Centre 1, Maitland Place Baulkham Hills NSW 2153 tel. (61) 2 8852 4700 fax (61) 2 8852 4777 Colombia bioMérieux Colombia Ltda Avenida 15 No.
Norway bioMérieux Norge AS Økernveien 145 N-0513, Oslo tel. (47) 23 37 55 50 fax (47) 23 37 55 51 Spain bioMérieux España S.A. Manual Tovar, 45–47 28034 Madrid tel. (34) 91 358 11 42 fax (34) 91 358 06 29 Philippines (The) Representation Office bioMérieux Philippines 11th Floor, Pearlbank Centre 146 Valero Street, Salcedo Village 1227 Makati City tel. (632) 817 7741 fax (632) 812 0896 Sweden bioMérieux Sverige AB Hantverksvägen 15 436 33 Askim tel.
Liability Disclaimer bioMérieux, Inc. makes no express or implied warranty regarding this manual, its quality, performance, or appropriate use regarding any type of specific procedure. Furthermore, this manual may be modified by bioMérieux without notice and without implying any obligation or liability on the part of the company. Intellectual Property bioMérieux, the blue logo, bioLIAISON and VITEK are used, pending and/or registered trademarks belonging to bioMérieux SA or one of its subsidiaries.
Warranty Seller, bioMérieux, Inc., warrants the VITEK® 2 instrument (the “instrument”) to the original purchaser for a period of one (1) year after date of installation against defects in material and workmanship and defects arising from failure to conform to specifications applicable on the date of installation.
STANDARD SYMBOLS The following table presents symbols that may appear in the instructions for use or on the instrument, package inserts, or packaging.
Standard Symbols Electric shock warning Radiation warning Potential pinch-point warning Laser Temperature limitation Upper limit of temperature Lower limit of temperature In Vitro Diagnostic Medical Device Batch code Authorized Representative in the European Community Catalog number Serial Number Do not reuse Recyclable ii Manual Name 702357-3EN1 REV nn/nnnn
Standard Symbols Separate collection for waste electrical and electronic equipment Very toxic Corrosive Sodium azide Irritant Positive control Negative control Keep away from sunlight Protect from light This way up Do not stack Humidity limitation Manual Name 702357-3EN1 REV nn/nnnn iii
Standard Symbols Fuse Direct current Alternating current Both direct and alternating current Three-phase alternating current Earth (ground) terminal Protective conductor terminal Frame or chassis terminal Equipotentiality ON (supply) OFF (supply) ON (only for a component of the system equipment) OFF (only for a component of the system equipment) Equipment protected throughout by double insulation or reinforced insulation (Equivalent to Class II of IEC 536) iv Manual Name 702357-3EN1 REV nn/nnnn
GENERAL WARNINGS IMPORTANT: The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the VITEK® 2 instrument and the Smart Carrier Station. IMPORTANT: The configuration that you have purchased is adapted to the legislation and standards of the different countries it will be sent to. For this reason, it may differ from the one presented in this document.
General Warnings General Warnings BIOHAZARD WARNING This instrument may be involved with hazardous organism suspensions. This user manual does not purport to address all of the safety matters associated with the instrument’s use. It is the responsibility of the user of this instrument to establish and follow appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.
General Warnings WARNING This statement only applies to European countries with regard to the waste electrical and electronic equipment European directive: You can play an important role in contributing to reuse, recycling and other forms of recovery of waste electrical and electronic equipment. Sorting this type of waste significantly reduces potential negative effects on the environment and human health as a result of the presence of hazardous substances in electrical and electronic equipment.
General Warnings Laser Caution A laser caution label appears on the VITEK® 2 and VITEK® 2 XL at the following locations: VITEK® 2 VITEK® 2 XL • on the front access door • on the left front access door • on the inside panel behind the front access door • above the center front sliding door • behind the waste collection door • behind the right side waste collection door The label appears as shown here: CAUTION Laser light when open. DO NOT STARE INTO BEAM.
TABLE OF CONTENTS General Warnings ........................................................................................................i General Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii Laser Caution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iv List of Tables ............................................................................................................
Table of Contents When to Configure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Accessing Configuration Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 SCS Main Configuration Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Cassette ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Setting Cassette ID. . . . . . . . . . . . . . . . . . . . . . .
Table of Contents Pipettor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12 Displacement Pump Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-13 Filler Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sealer Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Test Card Incubation and Reading . . . . . . . . .
Table of Contents Dilution Mode Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5 Status Screen Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5 Monitoring Pipette Tips and Saline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-6 VITEK® 2 Menu System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7 Frequently Used Keys. . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of Contents Tracking Cassettes and Cards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-27 Batch Loads (Smart Carrier or Cassette Only Mode) . . . . . . . . . . . . . . . . . . . 6-28 Selecting Batch Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-28 Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-28 Starting a Batch Load . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of Contents Troubleshooting the VITEK® 2 Instrument ........................................................... 8-1 Error Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-3 Error Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4 Types of Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4 Status Screen Error Conditions . . . . . . . . . .
Table of Contents Appendix A: Hardware Specifications ..................................................................A-1 VITEK® 2 Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Mass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Environment. . . . . . . . . . . . . . . . . . . . . . .
Table of Contents xii VITEK® 2 Instrument User Manual 510731-10EN1
LIST OF TABLES Table 2-1: Physical Characteristics .....................................................................................2-9 Table 2-2: Electrical Requirements .....................................................................................2-9 Table 2-3: Environmental Requirements .............................................................................2-9 Table 2-4: Bar Code Scanner Model References .............................................................
List of Tables xiv VITEK® 2 Instrument User Manual 510731-10EN1
LIST OF FIGURES Figure 2-1: Smart Carrier Station .........................................................................................2-2 Figure 2-2: SCS – Cable Connections .................................................................................2-4 Figure 2-3: Initial SCS Screen ..............................................................................................2-6 Figure 2-4: The Smart Carrier Station ..........................................................................
List of Figures Figure 5-6: Option Box Screen .......................................................................................... 5-12 Figure 5-7: Range Value Screen ....................................................................................... 5-12 Figure 6-1: VITEK® 2 Status Screen in Cassette Only Mode .............................................. 6-3 Figure 6-2: VITEK® 2 Status Screen in Non Standard Card Reading Mode .......................
List of Figures Figure 7-15: Molded Incubator Access Cover ......................................................................7-16 Figure 7-16: Carousel Cleaning Replace Section Now Screen ............................................7-17 Figure 7-17: Orientation of the Carousel ..............................................................................7-17 Figure 7-18: Removing the Button Memory Module .............................................................
List of Figures xviii VITEK® 2 Instrument User Manual 510731-10EN1
HOW TO USE T HIS MANUAL 1 Introduction This chapter gives you important information about how to use this manual. bioMérieux recommends that you read this chapter first. IMPORTANT: Read this manual carefully before you attempt to operate the VITEK® 2 system.
Organization How To Use This Manual Organization The manual is separated into four basic parts: • Part 1, which includes Chapter 2 and Chapter 3, covers the Smart Carrier Station. Chapter 2 describes the hardware and Chapter 3 provides the procedures you use to configure this station for your laboratory workflow. • Part 2, which includes Chapter 4 and Chapter 5, covers the VITEK® 2 Instrument. Chapter 4 describes the hardware and Chapter 5 shows you how to configure the instrument’s user interface.
How To Use This Manual How to Find Topics and Procedures How to Find Topics and Procedures There are four tools to help you find a topic or procedure in the manual. • General Table of Contents. This table is located in the front of the manual and includes the entire document. You can use this table to locate major headings throughout the manual. The table also includes a List of Tables and a List of Figures. • Chapter Table of Contents.
Typographic and Usage Conventions How To Use This Manual Example: File > Quit menu command Names and Titles The names and titles of menus, dialog boxes, fields, icons and toolbar buttons are in proper case, bold. Example: Setup menu The names of windows are in proper case, but are not bold. Example: Configuration window Press The term “press” refers to holding down a key on the keyboard in order to initiate action in the software.
How To Use This Manual Typographic and Usage Conventions Warnings, Cautions, and Information This manual uses different types of symbols to alert you to important information. Symbols and their associated information are labeled in text where they occur and set off from surrounding paragraphs, as shown in the following examples. WARNING Warning is a statement that alerts the user to the possibility of injury, death, or other serious adverse reactions associated with the use or misuse of a device.
Typographic and Usage Conventions 1-6 How To Use This Manual VITEK® 2 Instrument User Manual 510731-10EN1
SMART CARRIER STATION 2 Introduction Use this chapter to familiarize yourself with the Smart Carrier hardware. It includes an overview of each part of the unit.
Purpose Smart Carrier Station Purpose The purpose of this chapter is to aid in the safe handling, setup, configuration and operation of the Smart Carrier Station. Unpacking Instructions Figure 2-1 shows a Smart Carrier Station (SCS).
Smart Carrier Station Unpacking Instructions Inspect the shipping container for external damage. If it has been damaged, inspect the SCS itself for damage. If the SCS has been damaged, file a claim with the shipper and notify bioMérieux, Inc. Check the packing list included with the VITEK® 2 System and verify that all required items are included in the shipment. Note: The packaging materials and shipping container should be retained for future transportation needs, if necessary.
Unpacking Instructions Smart Carrier Station Installation Procedure After completing the unpacking procedure, the SCS is ready to be placed in its permanent location. The SCS is intended for use on any normal, flat bench top, commonly found in a microbiology lab, away from direct sunlight. Some cables must be connected to the unit. Figure 2-2 shows the cable connections on the back of the unit.
Smart Carrier Station Preparations for Operation Preparations for Operation Place the SCS in its permanent location. WARNING Do not place the SCS in a location where it could be exposed to direct sunlight during operation. Position the instrument to meet the minimum clearance of 5 cm (2 in.) on all sides and 30.5 cm (12 in.) above the instrument to provide adequate space for placing cassettes onto the unit. Use this procedure to begin using the SCS.
Smart Carrier Station Overview Smart Carrier Station Figure 2-3: Initial SCS Screen 5) Note: When the SCS is ready to be used, the screen shown in Figure 2-3 will be displayed. If there is no display, switch the power OFF and restart at Step 2. If you have not yet customized your configuration settings, the Configuration screen is displayed. For information on changing the settings, see Chapter 3 in this Manual.
Smart Carrier Station Smart Carrier Station Overview 3 2 1 5 4 Figure 2-4: The Smart Carrier Station 1 — SCS Base Unit. The SCS base contains the microprocessor. There are connection ports on the back of the base for the keyboard and the bar code scanner. The top of the base is specifically shaped to accept a cassette. Metal contacts on the base connect the unit’s computer to a button memory chip on the cassette.
SCS Keyboard Smart Carrier Station keyboard is smaller than a standard size keyboard, so it can be stored under the Base Unit. 5 — External Power Supply. The SCS is powered by an external DC power supply. This power supply accepts 120 VAC to 240 VAC mains power via a detachable two conductor power cord. SCS Keyboard The SCS keyboard has a standard set of alphabetic and numeric keys. It also has several keys that are uniquely designed for use on the SCS. Figure 2-5: The SCS Keyboard Help.
Smart Carrier Station SCS Base Unit SCS Base Unit The base unit for the SCS contains the electronic components that make its data entry and storage functions possible. The power switch and cable connectors are located on the back panel of the base unit. The base unit is actually a small computer with its own processing unit, memory, and software program. However, the program in this computer is limited to the task of entering and storing data for a cassette.
Setup Bar Codes Smart Carrier Station Table 2-3: Environmental Requirements (Continued) Shipment and Storage Temperature: –20 ºC to +50 ºC Humidity: 20% to 80% (Non-Condensing) Minimum installation altitude: –100m Maximum installation altitude: 2000m WARNING Do not operate the SCS in an environment above 30 °C (86 °F) Setup Bar Codes The bar code scanner is programmed at the factory. Use these setup bar codes in the event that the Bar Code Scanner loses its settings.
Smart Carrier Station Setup Bar Codes IMPORTANT: 3) Review the last two digits of the serial number (1, Figure 2-6) to determine which generation of SCS is in use. 4) Press F2 to exit the Configuration screen. 5) Refer to Table 2-4 and print the applicable bar code figure to use to program the bar code scanner. Print the applicable bar code figure using a laser printer. 6) Align the scanner several inches away from the first bar code illustrated in the applicable figure.
Setup Bar Codes Smart Carrier Station Table 2-4: Bar Code Scanner Model References (Continued) SCS Common Name 2nd Generation or G2 3rd Generation or G3 2-12 SCS Part Number(s) 530001-3 530001-4 27203 27204 27209 Bar Code Reader Bar Code Reference Figure Model 3400 (Scanner ID Number 722106) P/N 531130-1 (Discontinued) Figure 2-8 Model 3800 (Scanner ID Number IT3800) (Programmed to AT Mode) P/N 514144-2 Figure 2-10 Model 3800 – Black Figure 2-10 VITEK® 2 Instrument User Manual 510731-10EN1
Smart Carrier Station 1 2 3 4 5 6 Setup Bar Codes Factory Default 7 Exit PGM mode 8 Enter Program Mode 9 Good Read Delay PC-XT Format Enter Program Mode Set Inter char delay 10 High 11 Exit PGM mode Code 0 (3-27) Code 2 (3-27) Figure 2-7: Bar Codes for Bar Code Scanner – A VITEK® 2 Instrument User Manual 510731-10EN1 2-13
Setup Bar Codes 1 2 3 4 5 6 Smart Carrier Station Factory Default 7 Exit PGM mode 8 Enter Program Mode 9 Good Read Delay PC-AT Format Enter Program Mode Set Inter char delay 10 High 11 Exit PGM mode Code 0 (3-27) Code 2 (3-27) Figure 2-8: Bar Codes for Bar Code Scanner – B 2-14 VITEK® 2 Instrument User Manual 510731-10EN1
Smart Carrier Station Setup Bar Codes Factory Defaults ~ 0 1 D E F A L T . 1 IBM/XT Interface ~ 0 1 P A P CR Suffix _ X T ; V S U F C R . 2 Intercharacter Delay 10ms (02) ~ 0 Good Read Medium Delay 1 D L Y C H R 0 2 ; G R D D L Y 2 .
Setup Bar Codes Smart Carrier Station Factory Defaults (AT Interface) ~ 0 1 D E F A L T . 1 CR Suffix Intercharacter Delay 10ms ~ 0 1 V S U F C R ; D L Y C H R 0 2 .
SCS CONFIGURATION 3 Introduction Configuration options allow you to operate the Smart Carrier Station in a number of different ways. This chapter explains not only how to set these options, but also how each option affects the data stored in the button memory of a cassette.
Configuration Overview SCS Configuration Configuration Overview When to Configure Set SCS configuration options before you insert a cassette and enter information for the test cards. When first turned on, the Configuration Screen is displayed if you have not customized your settings. Note: One exception to this is the Firmware Update screen. You do not need to use that screen until you receive instructions from bioMérieux to do so.
SCS Configuration SCS Main Configuration Options • Firmware Updates – Displays the current firmware version and includes options for uploading new software for the SCS. Accessing Configuration Screens Access the SCS Configuration screen by pressing F4, followed by the Previous or Next Slot key to switch to the other screens.
SCS Main Configuration Options SCS Configuration Setting Cassette ID 1) Press F4 to access the Configuration screen. 2) In the Cassette ID field, type up to three characters and press Enter. 3) Press F2 to exit the SCS Configuration screen. or Press Next or Previous Screen to access other configuration screens. Setup Technologist ID The Setup Technologist ID field allows you to enter your name at the SCS and transfers that information to the workstation.
SCS Configuration SCS Workflow Configuration Options SCS Workflow Configuration Options Figure 3-2: SCS Workflow Configuration Screen AST Dilution Mode An AST, or Antimicrobial Susceptibility Test, uses an inoculum that is specifically diluted according to the type of test card being used. These inocula can be prepared manually by the technologist, or prepared automatically by VITEK® 2 at the Dispenser/Pipettor station.
SCS Workflow Configuration Options SCS Configuration Erase Cassette The Cassette Erase function gives you the option of automatically erasing cassettes that have been processed by the VITEK® 2 instrument. Setting Cassette Erase 1) Press F4 to access the Configuration screen. 2) Press Next or Previous Screen to access the Workflow Configuration screen. There are two settings available, Automatic (default) and Verify First. • Select Automatic to automatically erase cassettes that have been processed.
SCS Configuration SCS Utilities 4) Press F2 to exit the Workflow Configuration screen. or Press Next or Previous Screen to access other configuration screens. SCS Utilities Figure 3-3: SCS Utilities Screen Language The SCS interface is programmed in limited languages. English is the default language setting. By setting this configuration option, you can work on the SCS in the language of your choice. Setting SCS Language 1) Press F4 to access the Configuration screen.
SCS Utilities SCS Configuration Audible Feedback The SCS custom keyboard does not have an audible click when you press a key. This option enables an audible click from the instrument every time you press a key on the SCS keyboard. Setting Audible Feedback 1) Press F4 to access the Configuration screen. 2) Press the Down Arrow key to move the cursor to the Enable Audible Feedback field. 3) In the Audible Feedback field, press 0 to turn the Audible Feedback off.
SCS Configuration SCS Firmware Update 2) Press the Down Arrow key to move the cursor to the Time field. 3) In the Hour field, enter the current hour, using a 24-hour format (for example, 9 PM = 2100). Press Enter to accept the new value. 4) Enter the current minutes. Press Enter to accept the new value. 5) Enter the current day. Press Enter to accept the new value. 6) Enter the current month. Press Enter to accept the new value. 7) Enter the current, four-digit year.
SCS Firmware Update SCS Configuration Receive Firmware Update – This field allows you to request a complete firmware update from the workstation. Request Specific File – This field allows you to request a specific file from the workstation. If the use of this field is neccessary, the appropriate procedure will be included in the instructions that were provided with the update media.
VITEK® 2 INSTRUMENT 4 Introduction This chapter describes the hardware systems in the VITEK® 2 instrument. External Instrument Components on page 4-2 points out the controls and connections found on the instrument’s exterior, and the doors by which you gain access to its interior. Instrument Hardware Components on page 4-5 describes the work stations and components in the instrument.
VITEK® 2 Instrument External Instrument Components External Instrument Components The following images contain a front and a left side view of the VITEK® 2 and VITEK® 2 XL instrument. Use the diagram to locate and familiarize yourself with the external controls and the access doors to the interior of the instrument.
VITEK® 2 Instrument External Instrument Components Controls, Access Doors and Connections CAUTION: All access doors should remain closed when processing cards. LASER WARNING All access doors and covers must remain closed when processing cards to avoid exposure to laser light. 1 — User Interface Screen and Keypad. This screen and keypad comprise the User Interface system. See page 4-18 for detailed information. 2 — Front Access Door.
Turning on the VITEK® 2 Instrument VITEK® 2 Instrument 9 — Saline Access Door. Provides access to the sterile saline bag. The door lifts from the front and stays in the open position. 10 — Center Front Sliding Door. VITEK® 2 XL only. The Cassette load/ unload door (3) must be opened before this door can be opened. Connections 11 — UPS Connection. This cable connector port connects VITEK® 2 to an uninterruptable power supply (UPS).
VITEK® 2 Instrument Instrument Hardware Components Instrument Hardware Components The VITEK® 2 instrument is an integrated system, combining the tasks of sample preparation, test card inoculation, and test card incubation and reading. The parts and functions of the VITEK® 2 instrument can best be described by following a test card through the phases of a typical processing cycle.
VITEK® 2 Instrument Instrument Hardware Components Cassettes BIOHAZARD WARNING The boat should be considered as potentially contaminated and should be handled appropriately. Qualified laboratory personnel should take the usual precautions necessary for infectious agents. The cassette is the main component of the test card transport system. It can hold up to 15 test cards with their inoculum test tubes.
VITEK® 2 Instrument Instrument Hardware Components 4 — Button Memory. For systems using a Smart Carrier Station, each cassette is fitted with a special memory chip, called the button memory. When a cassette is on a Smart Carrier Station, the button memory stores the information that you enter for each test card. This information is read by a station in VITEK® 2, which marks the memory chip as being read, allowing the cassette to be reused. 5 — Cassette Base.
VITEK® 2 Instrument Instrument Hardware Components Table 4-2: Indicator Light States (Continued) Light Status Error Condition and Resolution Off The cassette load door is locked and cannot be opened. Blinking A boat with an empty cassette has arrived at the station. The cassette load door is unlocked. Open the door and remove the cassette. When you close the door, the blinking stops. CAUTION: The VITEK® 2 test card transport system stops while the cassette load door is open.
VITEK® 2 Instrument Instrument Hardware Components 4 5 1 2 3 Figure 4-5: Boat Components A boat, shown in Figure 4-5, has particular features that support its three major functions: 1— Test Card Transport. The top surface of a boat forms a specially shaped well into which a cassette is placed. The shape of the well conforms to the base of a cassette, providing it with a secure platform on which to ride.
VITEK® 2 Instrument Instrument Hardware Components Investigational Use Only (IUO) flag — This tells VITEK® 2 if an experimental test card is being processed. Test Card Expiration Date — This date is transferred to the workstation, which prints it on the laboratory report. Test Card Lot Information and Sequence Number — This includes the test card’s lot number to provide manufacturing traceability. The sequence number uniquely identifies a test card.
VITEK® 2 Instrument Instrument Hardware Components Dispenser/Pipettor Station This station automatically prepares the organism dilution used for AST (Antimicrobial Susceptibility Test) cards. VITEK® 2 provides a significant time savings by ensuring that mated ID and AST cards are processed from the same sample. Dispenser The dispenser system, shown in Figure 4-6, delivers 2.5 mL of sterile saline into the test tube of each AST card in a cassette. 1 2 4 3 Figure 4-6: Dispenser System Sterile Saline.
VITEK® 2 Instrument Instrument Hardware Components chamber but is attached to an air pump. It is fitted with a filter (4) to prevent particulate contamination. Electromechanical Components (not shown). The electromechanical components control how the system works. 1) When an AST card is detected, a valve opens to allow saline to travel from its bag to the dispensing chamber. 2) An optical sensor detects when the chamber is full (2.5 mL) and closes the valve.
VITEK® 2 Instrument Instrument Hardware Components Note: The displacement pump is not normally visible as it is located directly behind the interface screen and keypad. Displacement Pump Operation The displacement pump is part of a larger assembly that performs the pipetting and transfer of the suspension when VITEK® 2 is in the Automatic dilution mode. Here is how it works: 1) The container drops a pipette tip into position.
Instrument Hardware Components VITEK® 2 Instrument Various temperature and air pressure sensors in the system monitor the inside of the vacuum chamber. VITEK® 2 ensures proper test card fills by monitoring these parameters throughout the entire cycle and controlling the rate at which the vacuum is drawn and released. Note: The seal on the vacuum chamber should be cleaned periodically. See the maintenance schedules and procedures in Chapter 7 of this manual.
VITEK® 2 Instrument Instrument Hardware Components Carousel The Carousel, shown in Figure 4-8, has a capacity of 60 test cards. During their time in the carousel, the test cards are incubated at an average temperature of 35.5 °C. Figure 4-8: Carousel Placement As the carousel rotates, each test card moves into the reading position every 15 minutes. A mechanical device called the Reader Head, shown in Figure 4-9, conveys the test card through the optics stations.
VITEK® 2 Instrument Instrument Hardware Components Optics The VITEK® 2 instrument performs its identification and susceptibility analyses by continually monitoring the growth and activity of organisms inside the wells of the test cards. The optics system performs this function. Transmittance Optics The transmittance optics (1, Figure 4-10) use visible light to directly measure organism growth. These optics are based on an initial light reading of a well before significant growth has begun.
VITEK® 2 Instrument Instrument Hardware Components CAUTION: Do NOT re-insert ejected test cards into the VITEK® 2 instrument. WARNING Make sure that all test card processing has been completed before ejecting a test card. Waste Collection Station The card ejector removes test cards that have completed testing from the reader. These test cards are collected in a tray at the Waste Collection Station for removal from the VITEK® 2 instrument and disposal.
VITEK® 2 Instrument User Interface System Note: Periodically remove the waste collection tray for cleaning. See Cleaning the Test Card Collection Tray on page 7-25. User Interface System Throughout the entire processing cycle for test cards, communication between the user and the VITEK® 2 instrument is essential. The VITEK® 2 User Interface System provides the means of that communication. Keypad and Screen A keypad and screen are located on the front of the instrument.
VITEK® 2 Instrument User Interface System 2 — Help (?) Key. Press this key at any time to access the error/message queue. 3 — Previous Screen Key. Use this key to: • • • • Exit from a screen or function to its menu. Return to a previous screen in a function. Go from a sub-menu to its previous menu. Go from the Main Menu to the Status screen. 4 — Undo Key. Use it to cancel the last action performed. 5 — Arrow Keys. Use these keys to: • Scroll a screen or menu. • Move the cursor on screen.
User Interface System 4-20 VITEK® 2 Instrument VITEK® 2 Instrument User Manual 510731-10EN1
CONFIGURING THE VITEK® 2 INSTRUMENT 5 Introduction Configuration options allow you to operate the VITEK® 2 instrument in a number of different ways. This chapter explains not only how to set these options, but how each option affects the operation of the instrument, and therefore your laboratory workflow. Certain configuration options affect only the physical interface and can be changed to suit your preferences. For example, you can adjust the contrast on the interface screen.
Configuring the VITEK® 2 Instrument Configuration Options Configuration Options Configuration Overview There are 12 configuration options in the VITEK® 2 user interface. They can be divided into two groups, depending on whether or not the option affects how the instrument operates.
Configuring the VITEK® 2 Instrument Configuration Options 2) Evaluate the effect that an option has on your workflow and decide how to set the option. This is especially important for the options in Table 5-1. 3) Set the options according to the choices made in Step 2, by following the procedures in this chapter. 4) Operate the system to validate the settings and evaluate the physical parameter settings for the options in Table 5-2. 5) Change any option as required.
Configuring the VITEK® 2 Instrument Configuration Options For information on setting this option, see the procedure Defining Character Sets on page 5-9. Schedule Instrument QC Status The QC Status is a report of the current incubator temperature and the status of the optics systems. The report is sent to the VITEK® 2 workstation where it is recorded with the date and time the report was taken.
Configuring the VITEK® 2 Instrument Configuration Options Recording QC Report The instrument user interface contains a function with which you can manually record a QC Status. You can set this option if you want the report recorded automatically. For example, you can have the report recorded at the same time every day. For information on setting this option, see the procedure Setting Time for QC Status on page 5-10.
Configuring the VITEK® 2 Instrument Configuration Options Note: If the instrument is in Cassette Only mode, you can use the Cassette Setup function (Main Menu) to process individual cassettes in the dilution mode opposite to the configuration setting. BIOHAZARD WARNING Biohazardous spills can occur inside the VITEK® 2 instrument if the Dilution Mode option is not properly set. This is especially true if pre-diluted samples are used with the mode set to Automatic.
Configuring the VITEK® 2 Instrument Configuration Options The default setting for the alarm volume is at the midpoint of its range. Use this option to make the appropriate adjustments as required. The option includes a test function so that you can hear the alarm while changing it. For information on setting this option, see the procedure Setting a Range Value on page 5-12. Visual Alarm Enable The instrument causes the interface screen display to blink to alert you to an error condition.
Configuring the VITEK® 2 Instrument Using the Interface For information on setting this option, see the procedure Setting a Range Value on page 5-12. Waste Tray Warning Level The VITEK® 2 instrument displays a warning message when the Waste Collection Tray reaches full capacity. Use this setting to adjust the amount of advance warning the instrument will provide.
Configuring the VITEK® 2 Instrument Using the Interface All of the configuration options for VITEK® 2 are on the Configuration menu. You access this menu using the path: Main Menu > Utilities > Configuration The VITEK® 2 Main Menu looks like this: Figure 5-2: VITEK® 2 Main Menu Defining Character Sets Use this procedure for the Cassette Names and Instrument Name configuration options.
Configuring the VITEK® 2 Instrument Using the Interface Note: This screen does not appear for the Instrument Name option. 3) Press a number key to select one of the ten cassette name positions, and then press Enter. The Character Selection screen appears: Figure 5-4: Character Selection Screen Note: This screen is similar for both the Cassette Names and Instrument Name options. 4) Select a letter using the Arrow keys and then press the option button for the character box.
Configuring the VITEK® 2 Instrument Using the Interface The following screen appears: Figure 5-5: Schedule QC Status Screen The time fields operate on a 24-hour clock. Times A, B, or C are disabled until you schedule the QC Status. Note: The Left or Right Arrow keys beneath Hours/Minutes allow you to toggle between Hours and Minutes when setting the time. To set the Hour for Time A: 1) Press the Left Arrow key until Hours displays. 2) Use the Up or Down Arrow keys to set the hour.
Configuring the VITEK® 2 Instrument Using the Interface 2) Press Up or Down Arrow to choose an option. A screen similar to Figure 5-6 appears. Figure 5-6: Option Box Screen 3) To change the current setting, press the Up or Down Arrow keys.
PROCESSING VITEK® 2 TEST CARDS 6 Introduction This chapter contains the procedures you need to process VITEK® 2 test cards. The chapter begins with three introductory sections that provide reference information about the VITEK® 2 Status screen, the VITEK® 2 Menu system, and the Smart Carrier Station interface. The next two sections contain suggested workflows and the procedures for working either with or without a Smart Carrier Station (SCS).
Processing VITEK® 2 Test Cards Cassette Only Mode Workflow • 6-21 Cassette Preparation • 6-22 Loading a Cassette • 6-25 Entering Data for a Cassette • 6-27 Batch Loads (Smart Carrier or Cassette Only Mode) • 6-28 Selecting Batch Loading • 6-28 Starting a Batch Load • 6-28 Unloading the Cassette and Removing Waste • 6-30 Unloading a Cassette • 6-30 Removing Ejected Test Cards • 6-31 6-2 VITEK® 2 Instrument User Manual 510731-10EN1
Processing VITEK® 2 Test Cards Instrument Status Screen Instrument Status Screen You use the Status screen more often than any other screen in the VITEK® 2 user interface. Since it is so basic to the workflow, the Status screen is very easy to display and in many cases, the interface displays it automatically. Displaying the Status Screen There are three ways to display the Status screen: • Automatically displays at the end of the instrument’s power up initialization process.
Processing VITEK® 2 Test Cards Instrument Status Screen • Messages. This status indicates that there is an error message in the error/message queue that has not been viewed. Press the Help (?) key to view the error/message queue. • Errors. This status appears after the error/message queue is accessed if the error condition has not been resolved. This status can be cleared only by resolving the condition that generated the original message. • Cleaning.
Processing VITEK® 2 Test Cards Instrument Status Screen The number you see in the MAX Available Slots field depends on the VITEK® 2 instrument you have and the optical configuration of the instrument.
Processing VITEK® 2 Test Cards Instrument Status Screen •VITEK® 2 Instrument Icon. This icon represents the VITEK® 2 instrument. It shows when one of the access doors is open. These include the front access door on the front of the instrument, and the top access door. •VITEK® 2 XL Instrument Icon. This icon represents the VITEK® 2 XL instrument. It shows when one of the access doors is open. These include the Section A top access door, Section B top access door, and the center front sliding door.
Processing VITEK® 2 Test Cards Instrument Status Screen Figure 6-3: Pipette Tips Status As the disposables are used, the bar graphs begin to decrease. The graphs are marked to show levels of ¼, ½, and ¾ capacity. The number associated with the graph (156, in this example) decreases at the same time. When the number goes under 40, the value changes to Low. Note: For pipette tips, the number refers to the number of available tips. For saline, the number refers to the available number of aliquots.
6-8 Cassette Names Instrument Name Boat Cleaning Change Saline Display Instrument QC Status Schedule QC Status Carousel Cleaning Cleaning (Menu) Change Pipette Tips Maintenance (Menu) Cassette Mode Dilution Mode Optics Cleaning Shut Down Cassette Setup Optics Audible Alarm Enable Audible Alarm Volume Card Transport Visual Alarm Enable Vacuum Move Boat Waste Tray Warning Level Dispenser/ Pipettor (Instrument) Diagnostics (Menu) Utilities (Menu) Figure 6-5: Detailed Structure of the
Processing VITEK® 2 Test Cards About the Smart Carrier Station (SCS) In Figure 6-5, the menu structure is shown with all of the function options added. Bold lines show the menus and pathways between them. This manual describes the pathway to a specific function by naming each menu to access, with the menus separated by arrows.
About the Smart Carrier Station (SCS) Processing VITEK® 2 Test Cards Type, Organism, Offline Tests, and Modifier fields can be entered without having to use the keyboard. If your laboratory uses bar codes for accession number of specimens, these can also be entered using the scanner. Cassette. The cassette helps you organize your work. Each cassette has 15 slots for test cards and 15 corresponding wells for specimen test tubes.
Processing VITEK® 2 Test Cards About the Smart Carrier Station (SCS) discrepancies in test card type or position in the cassette are reported to the user so that corrective action can be taken. Cassette Edit Screen The Cassette Edit screen provides an interface for entering test information. This screen is repeated for each cassette slot. Figure 6-7: Fields of the Cassette Edit Screen • Cassette ID.
About the Smart Carrier Station (SCS) Processing VITEK® 2 Test Cards • Modifier. Field used for organism modifiers. Six generic entries, mod1 to mod6, are preloaded. When this data reaches the workstation computer, the generic entry is converted to one of the first six corresponding entries in the Organism Modifier table of the bioLIAISON® database. • Dilution Mode. The current dilution mode setting is displayed to allow quick verification.
Processing VITEK® 2 Test Cards About the Smart Carrier Station (SCS) SCS Functions Table 6-2 summarizes the functions that can be performed while entering data, and the use of the special function keys. Table 6-2: Summary of SCS Functions Key Name Function(s) How To Use It Help (?) Display context-sensitive help screens and data field option boxes. Press Help (?). Up/Down or Left/ Right Arrow key Move between fields. Press the Up/Down or Left/Right Arrow keys. Access the Isolate Number field.
Processing VITEK® 2 Test Cards About the Smart Carrier Station (SCS) Table 6-2: Summary of SCS Functions (Continued) Key Name Function(s) F9 Erase the data for a slot. Note: This function also erases data in any successive linked slots. F10 Erase all the data in the button memory chip. How To Use It 1) Press F9. A confirmation window appears. 2) Press F1 to erase the data. 1) Press F10. A confirmation window appears. 2) Press F1 to erase the button memory chip.
Processing VITEK® 2 Test Cards About the Smart Carrier Station (SCS) Offline Tests > blac + blac Figure 6-9: Offline Tests Option Box ANC Identification Card Offline Test Result Screen The Offline Test Result screen allows entry of values that are specific to Anaerobic and Corynebacteria (ANC) tests. Use the Up and Down keys to change fields. Figure 6-10: Offline Test Result Screen Gram The Gram field provides an option box that allows the entry of Gram stain information for the current test.
Processing Test Cards Using the Smart Carrier Station Processing VITEK® 2 Test Cards Modifier The option box for the Modifier field contains six default entries, mod 1 to mod 6. When this data reaches the workstation computer, the entry is matched to one of the first six entries in the Organism Modifier table of the bioLIAISON® database. Selecting an Entry Using an Option Box 1) Use the Up or Down Arrow key to move the cursor into the correct field.
Processing VITEK® 2 Test Cards Processing Test Cards Using the Smart Carrier Station • On the VITEK® 2 Instrument. The most important configuration option for using the SCS is the Cassette Mode. This option must be set to Smart Carrier. Smart Carrier Workflow Table 6-3 provides a general workflow for using the SCS.
Processing VITEK® 2 Test Cards Processing Test Cards Using the Smart Carrier Station The SCS displays the Cassette Edit screen (see Figure 6-7) automatically whenever a cassette is firmly seated on the SCS base unit. 2) Select the correct slot. • Make sure that the Cassette Edit screen is displaying the correct slot (1–15). Use the Next Slot or Previous Slot keys. • Make sure that the cursor (blinking bar) is in the correct field. Use the Up or Down Arrow keys. 3) Enter the data as shown in Table 6-4.
Processing VITEK® 2 Test Cards Processing Test Cards Using the Smart Carrier Station • A Gram negative inoculum can be used for either one or two susceptibility tests. When diluting the inocula for two tests, the test cards must occupy the two slots immediately following the slot containing the inoculum test tube. • The VITEK® 2 AST card type (e.g., AST-P515) MUST be entered in the Card Type field on the Smart Carrier Station Cassette Edit screen.
Processing Test Cards Using the Smart Carrier Station Processing VITEK® 2 Test Cards Before you attempt to load a cassette, make sure the indicator light is on or is blinking. Loading a Cassette 1) Open the Cassette Load Station by lifting the cassette load door up. There should be an empty boat at the station, or one with a cassette that contains waste. CAUTION: Do not attempt to load a cassette if a boat is not at the station. Damage to the cassette and the VITEK® 2 instrument is possible.
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode information is provided once the test cards reach the Card Incubator and Reader Station and are read for the first time. Note: The VITEK® 2 instrument should be attended during the first minute after a cassette is loaded and the boat moves forward to process cards. This period is denoted by the presence of the Cassette icon on the VITEK® 2 Status screen. See Cassette Load Processing Errors on page 8-10 for additional information.
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode • selected the VITEK® 2 test cards to process • decided whether to prepare susceptibility suspensions manually or automatically using VITEK® 2 Note: If you choose to prepare suspensions manually, suspension preparation instructions can be found in the VITEK® 2 Systems Product Information. Table 6-7: Processing Test Cards (Cassette Only Mode) Activity Reference(s) 1) Make sure that all configuration options are set correctly.
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode Date: 07/26/2005 08:38:23 WSVT2-R04.
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode Correct Positioning of Susceptibility Test Cards If you pre-dilute the inocula for the susceptibility tests, you can place the test cards into any slots in the cassette, in any order you choose. However, when VITEK® 2 is performing the dilution (Automatic Dilution mode), you must follow these rules: • The AST card must be placed in a slot immediately following the slot containing the inoculum test tube.
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode 2) Select one of the cassette names (1, Figure 6-13) from the list shown by pressing its corresponding number on the keypad. The selected name is highlighted. Note: If you need to add a new cassette name, refer to the section Cassette Names on page 5-3. 3) If necessary, press the Change Dilution Mode button (3) to change the current dilution mode (2).
Processing VITEK® 2 Test Cards Processing Test Cards in Cassette Only Mode Table 6-8: Cassette Load Parameters (Cassette Only Mode) (Continued) Parameter Tips and Saline What to Look For The level of both disposables should be greater than or equal to the number of test cards you are loading. Note: This parameter may be ignored if you are operating in the manual dilution mode. Cassette Name The name of the previously used cassette is shown, and will be applied to this cassette unless you change it.
Processing VITEK® 2 Test Cards Note: Processing Test Cards in Cassette Only Mode The boat is shaped such that it will only accept a cassette in the proper orientation. 3) Close the cassette load door. The Cassette icon appears on the Status screen. 4) Monitor the instrument until the Cassette icon disappears and the boat moves forward to process cards. Monitoring Test Card Processing After a cassette is loaded, the test cards in it are scanned by the bar code reader in VITEK® 2.
Processing VITEK® 2 Test Cards Batch Loads (Smart Carrier or Cassette Only Mode) Table 6-9: Card Identity Components (Cassette Only Mode) (Continued) Identifier Example Source Slot position (1–15) of the test card in the cassette. 03 The actual test card position in the cassette as read by the VITEK® 2 bar code reader. Load Time Apr 03 08:35:40 The date and time the test card was read by the VITEK® 2 bar code reader.
Processing VITEK® 2 Test Cards 2) Batch Loads (Smart Carrier or Cassette Only Mode) Access the Batch Load function on the Main Menu. The VITEK® 2 instrument checks to ensure that at least three boats are available, and that there are no test cards currently in the card transport system. It then displays the following screen: Figure 6-15: Batch Load Screen Note: This screen does not appear if there are only three boats in the instrument. In that case, a batch load of three cassettes is assumed.
Unloading the Cassette and Removing Waste Processing VITEK® 2 Test Cards Figure 6-17: Cassette Only Mode Batch Load Screen 4) Open the door to the Load Station, place the cassette in the boat, and close the door. The instrument moves the boat away from the Load Station, moves the next boat into the station, and redisplays the screen shown in the previous step. 5) Repeat Step 4 as required for the other cassettes.
Processing VITEK® 2 Test Cards Unloading the Cassette and Removing Waste For more information about the indicator light, see the section Using the Cassette Load Station on page 6-19 or on page 6-26. 2) Note: Open the cassette load door and remove the cassette from the boat. The boat itself should not be removed. 3) Close the cassette load door. CAUTION: Leaving the door to the Cassette Unload station open will interfere with the proper processing of the test cards in the VITEK® 2 instrument.
Processing VITEK® 2 Test Cards Unloading the Cassette and Removing Waste 1 2 Figure 6-18: The Waste Collection Station Removing the Waste Collection Tray Note: 1) Open the Waste Collection Station door. Note that waste test cards are held in a removable tray. 2) Place the index finger of one hand on the sliding retainer bar (1, Figure 6-18) to prevent it from snapping back.
MAINTAINING THE VITEK® 2 INSTRUMENT 7 Introduction This chapter includes the procedures required to maintain the VITEK® 2 instrument. Maintenance includes monitoring and changing the disposables, cleaning the boats and the carousel, and general maintenance and cleaning. Note: The Maintenance Log provided at the end of this chapter lists recommended maintenance procedures and their frequency. Please make one copy for your use and keep the original with this manual.
Maintaining the VITEK® 2 Instrument Cleaning the Drip Pan • 7-30 Replacing the Drip Pan • 7-31 Turning the Instrument On • 7-31 Cleaning the SCS • 7-32 7-2 VITEK® 2 Instrument User Manual 510731-10EN1
Maintaining the VITEK® 2 Instrument Maintaining Disposables Maintaining Disposables Chapter 6 introduced the Status screen and how to incorporate it into your workflow. A specific part of that screen is devoted to the maintenance of the VITEK® 2 disposables. 1 2 Figure 7-1: VITEK® 2 Status Screen Monitoring the Disposables Note the two bar graphs at the bottom of the screen. The Tips graph (1, Figure 7-1) displays the current level of pipette tips that remain in the instrument.
Maintaining the VITEK® 2 Instrument Maintaining Disposables Using the Graphs The best way to use the graphs is to periodically compare the levels of the graphs to the number of ASTs you anticipate during a period. If the disposables level is lower than the anticipated work load, install an accessory kit before you begin (see following sections). In this way, your workflow will not be interrupted during test card processing.
Maintaining the VITEK® 2 Instrument 3) Maintaining Disposables Open the front access door (1, Figure 7-2) to expose the Dispenser Station, then lift the saline access door (2) to expose the saline bag. 2 3 1 5 4 Figure 7-2: Location of Saline Dispensing Chamber Note: 4) Pull on the filter disk (3) to remove it from its connector. 5) While pushing the Dispenser Release button (4), carefully pull the shot tube (5) out of its housing. 6) The entire assembly is now disconnected.
Maintaining the VITEK® 2 Instrument Maintaining Disposables 6 7 Figure 7-3: Cannula and Tubing Assembly 11) Place the new bag of saline on its shelf on the top of the instrument, placing the eyelet of the bag over the post at the back of the shelf. 12) While pushing the release button (8, Figure 7-4), insert the saline dispenser tube (9) into its housing. After the tube is fully inserted, release the button.
Maintaining the VITEK® 2 Instrument Maintaining Disposables 12 Figure 7-5: Saline Fill Line 15) Release the round plate (11, Figure 7-4). 16) Close both the front access door and the saline access door to the instrument. 17) Press Done. The following message appears: Performing Dispenser Self Test The self test checks for proper installation of the saline dispenser tube. It is recommended that you wait for the test to complete (about 20 seconds) before leaving the instrument.
Maintaining the VITEK® 2 Instrument Maintaining Disposables Installing an Accessory Kit, Part B: Pipette Tips If the card transport system is processing cassettes through the Card Transport Station, the following message appears: Card Transport System is busy. Please try later. CAUTION: Use aseptic technique when installing an accessory kit.
Maintaining the VITEK® 2 Instrument Maintaining Disposables 7) Remove the pipette container from its base using a pulling motion. 8) Discard any pipette tips that remain in the container. CAUTION: To avoid contaminating new pipette tips, do not touch the inside of the pipette container. Note: Discarding the remaining tips is required in order for VITEK® 2 to maintain an accurate count of them. This action also minimizes the risk of contamination.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel The following message appears: Performing Pipettor Self Test The Pipettor self test only checks the electric motors in the Pipettor Station. If the motors pass the test, no further message is displayed, and you are done with the installation. If a problem is found, the following message appears: Pipette Tip installation error When this message appears, call bioMérieux for assistance.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel Figure 7-8: VITEK® 2 XL Carousel Cleaning Screen If cards are not processing in the selected incubator, and the carousel sections have not been removed, the following screen displays: Figure 7-9: VITEK® 2 XL Preparing for Section Removal Screen Note: When the Continue button is pressed, you are required to remove all of the sections. This will require about four minutes.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel Note: There are two types of incubator access covers for the VITEK® 2 instrument. Perform the first part of Step 5 if the machined incubator access cover is in use. Perform the second part of Step 5 if the molded incubator access cover is in use.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel 3 Figure 7-11: Molded Incubator Access Cover When you remove the cover, the message on the screen changes to: Preparing for Section Removal 6) When the carousel is in position, the following screen displays: Figure 7-12: Carousel Cleaning Prompt 7) VITEK® 2 Instrument User Manual 510731-10EN1 The carousel is removed in four sections. Grasp the first carousel section and pull it slightly forward (toward the front of the instrument).
Maintaining the VITEK® 2 Instrument Cleaning the Carousel Figure 7-13: Removing a Carousel Section 8) Press the Press here after completion option button to indicate removal of the carousel section. The following message displays: Current number of sections: 3 of 4 The carousel turns 90° so that the next section can be removed. During this time the message Preparing for Section Removal displays again on the screen.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel Carousel Cleaning Methods The four sections of the carousel should be thoroughly cleaned and dried before they are replaced in the VITEK® 2 instrument. The carousel material is designed to withstand any of the following cleaning methods: • Automatic dishwasher with standard laboratory detergent CAUTION: Dishwasher temperatures during the washing and drying cycles must not exceed 85 °C (185 °F).
Maintaining the VITEK® 2 Instrument Cleaning the Carousel 3) Remove the incubator access cover (1, Figure 7-14) from the carousel by holding the retaining pin (2) on the top of the cover to the right while pulling the cover slightly forward and to the right, out of the instrument. 2 1 Figure 7-14: Machined Incubator Access Cover or Remove the incubator acces cover (3, Figure 7-15) from the carousel by lifting the top of the cover straight up from the instrument.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel When the access cover has been removed, the following message displays: Preparing for section replacement When the carousel is in position, the following screen displays: Figure 7-16: Carousel Cleaning Replace Section Now Screen 4) Take any one of the carousel sections and orient it as shown in Figure 7-17.
Maintaining the VITEK® 2 Instrument Cleaning the Carousel 6) When the section has been pushed down all the way, release the top portion, allowing it to rest against the base plate. When you perform Step 6, two pins on the back of the section should engage the two holes (4) in the base plate and secure the section. 7) Press the Press here after completion option button to indicate that the section is in place.
Maintaining the VITEK® 2 Instrument Cleaning the Cassettes Cleaning the Cassettes Cassettes should be cleaned monthly, or as conditions require. BIOHAZARD WARNING The cassettes should be considered as potentially contaminated and should be handled appropriately. Qualified laboratory personnel should take the usual precautions necessary for infectious agents. CAUTION: Exposing the button memory to the recommended cassette cleaning procedures can cause irreparable damage to the unit.
Maintaining the VITEK® 2 Instrument Cleaning the Cassettes 1 2 Figure 7-18: Removing the Button Memory Module 4) Clean the metal contacts (3, Figure 7-19) on the module with an alcohol wipe. 3 Figure 7-19: Metal Contacts on Cassette Note: When cleaning multiple cassettes, it is not necessary to match a button memory module to a particular cassette. The units are completely interchangeable. 5) The cassettes should be thoroughly cleaned and dried before they are used again.
Maintaining the VITEK® 2 Instrument Cleaning the Boats 1 2 4 3 Figure 7-20: Replacing the Button Memory Module 3) Place the other end of the module against the holding bracket (4) and push the module towards the side of the cassette. The module should snap into place. Cleaning the Boats Note: The procedures in this section should be performed monthly, or as conditions require.
Maintaining the VITEK® 2 Instrument Cleaning the Boats Figure 7-21: Boat Cleaning Prompt 2) Press Remove Boat. If there are boats in the card transport system, and it is not idle, the instrument displays the message: Card transport system is busy. Please try later. If the system is idle, the instrument moves a boat to the boat dock while displaying this message: Moving Boat to Boat Dock. When the boat is ready for removal, the following message appears: Boat is at Boat Dock. Please remove it.
Maintaining the VITEK® 2 Instrument Cleaning the Boats Cleaning the Boats The four boats should be thoroughly cleaned and dried before they are replaced in the VITEK® 2 instrument. The boat material is designed to withstand any of the following cleaning methods. • Automatic dishwasher with standard laboratory detergent CAUTION: Dishwasher temperatures during the washing and drying cycles must not exceed 85° C (185° F). Exceeding this temperature will cause damage to the boats.
Maintaining the VITEK® 2 Instrument Cleaning the Instrument Interior • The front legs (1, Figure 7-22) of the boat are positioned in the channel of the base pan. • The boat is oriented with the arrow (2) pointing toward the back. 2 1 Figure 7-22: Positioning of Boat 6) Press the Press here after completion option button shown below after replacing the boat. The first screen reappears with the boat count at 1 of 4. 7) Repeat this procedure, starting at Step 2, until all four boats have been replaced.
Maintaining the VITEK® 2 Instrument Cleaning the Instrument Interior CAUTION: There are elevated temperatures and hot surfaces inside the instrument. Shut down the instrument before starting the cleaning or any other procedures inside the instrument. Shutting Down the Instrument Note: Finish processing all cards before beginning this procedure.
Maintaining the VITEK® 2 Instrument Cleaning the Instrument Interior The tray material is designed to withstand any of the following cleaning methods: • Automatic dishwasher with standard laboratory detergent CAUTION: Dishwasher temperatures during the washing and drying cycles must not exceed 85 °C (185 °F). Exceeding this temperature will cause damage to the cassettes. Use the top rack of the dishwasher.
Maintaining the VITEK® 2 Instrument Cleaning the Instrument Interior 1 Figure 7-23: Optics The optics consist of multiple units that are hinged on the bottom and held in place by spring-loaded levers. 3) Using your right hand, grasp the optics unit and place your thumb on the lever (2, Figure 7-24). 2 Figure 7-24: Opening the Optics Note: 4) Push down on the lever to release the unit and allow it to rotate on its hinges. 5) Inspect the glass on both surfaces for cracks or scratches.
Maintaining the VITEK® 2 Instrument Cleaning the Instrument Interior 9) Replace the empty test card collection tray in the Waste Collection Station. 10) Close the top access door. 11) Close the Waste Collection door. Note: After cleaning the optics and returning power to the instrument, perform the Optical Diagnostic Test on page 8-26. If you are not performing the remaining cleaning procedures in this section, skip to the procedure Turning the Instrument On on page 7-31.
Maintaining the VITEK® 2 Instrument Cleaning the Drip Pan 1 Figure 7-25: Vacuum Seal 1) Using a solution of disinfectant and warm water, wipe off any dust or dirt from the surface of the base pan. Particular attention should be paid to the area beneath the Dispenser/ Pipettor Station, where drips or spills may have occurred. Note: Note: To disinfect a contaminated surface, use the 10% bleach solution and allow it to remain in contact with the contaminated surface for five minutes.
Maintaining the VITEK® 2 Instrument Cleaning the Drip Pan VITEK® 2 has a small container, called the drip pan, located underneath the Dispenser/Pipettor Station. The pan is designed to catch any spills that occur at this station. Note: This procedure should be performed after you clean the base pan. See Cleaning the Base Pan, Vacuum Seal and Vacuum Chamber on page 7-28. Removing the Drip Pan BIOHAZARD WARNING The contents of the drip pan may include biohazardous materials. Take appropriate precautions.
Maintaining the VITEK® 2 Instrument Cleaning the Drip Pan CAUTION: Dishwasher temperatures during the washing and drying cycles must not exceed 85 °C (185 °F). Exceeding this temperature will cause damage to the drip pan. Use the top rack of the dishwasher. • 10% bleach solution • Phenolic cleaning solution Note: To disinfect a contaminated surface, use the 10% bleach solution and allow it to remain in contact with the contaminated surface for five minutes.
Maintaining the VITEK® 2 Instrument Cleaning the SCS Cleaning the SCS BIOHAZARD WARNING All organism suspensions should be considered as potentially infectious. Qualified laboratory personnel should use acceptable procedures for biohazardous material. Note: This procedure should be performed monthly, or as conditions require.
Maintaining the VITEK® 2 Instrument Cleaning the SCS 5) Clean all surfaces of the SCS base unit, keypad, display, and bar code scanner, except the clear lens of the scanner. 6) Wipe the clear lens of the bar code scanner using a quality, lint free lens paper that has been moistened with a commercial glass cleaner. If foreign material remains on the surface, repeat this step using an alcohol wipe. 7) Allow all parts to dry thoroughly before reconnecting the power cord and switching the power on.
Cleaning the SCS 7-34 Maintaining the VITEK® 2 Instrument VITEK® 2 Instrument User Manual 510731-10EN1
Day of Month: 1 2 Month____________Year____________ 3 4 5 6 7 8 9 10 11 12 13 14 15 Change Saline A/R Change Pipette Tips A/R Clean Boats* Clean Carousel* 16 Maintaining the VITEK® 2 Instrument VITEK® 2 Instrument User Manual 510731-10EN1 VITEK® 2 Maintenance Log Clean Cassettes* Clean Optics* Clean Waste Tray* Clean Base Pan* Clean Vacuum Seal* Clean Vacuum Chamber* Clean Drip Pan* Check Instrument QC Status Report for the Carousel Temperature** Optics Systems** Clean Smart Carrier* *
Day of Month: 17 18 19 Month____________Year____________ 20 21 22 23 24 25 26 27 28 29 30 31 Change Saline A/R Maintenance Logs 7-36 VITEK® 2 Maintenance Log Change Pipette Tips A/R Clean Boats* Clean Carousel* Clean Cassettes* Clean Optics* Clean Waste Tray* Clean Base Pan* Clean Vacuum Seal* Clean Vacuum Chamber* Clean Drip Pan* Check Instrument QC Status Report for the VITEK® 2 Instrument User Manual 510731-10EN1 Optics System* Clean Smart Carrier* * = Monthly ** = Daily MASTER C
Day of Month: 1 2 3 Month____________Year____________ 4 5 6 7 8 9 10 11 12 13 14 15 16 Change Saline A/R Change Pipette Tips A/R Clean Boats* Clean Carousel A & B* Maintaining the VITEK® 2 Instrument VITEK® 2 Instrument User Manual 510731-10EN1 VITEK® 2 XL Maintenance Log Clean Cassettes* Clean Optics A & B* Clean Waste Tray A & B* Clean Base Pan* Clean Vacuum Seal* Clean Vacuum Chamber* Clean Drip Pan* Check Instrument QC Status Report for the Carousel Temp.
Day of Month: 17 18 19 Month____________Year____________ 20 21 22 23 24 25 26 27 28 29 30 31 Change Saline A/R Maintenance Logs 7-38 VITEK® 2 XL Maintenance Log Change Pipette Tips A/R Clean Boats* Clean Carousel A & B* Clean Cassettes* Clean Optics A & B* Clean Waste Tray A & B* Clean Base Pan* Clean Vacuum Seal* Clean Vacuum Chamber * Clean Drip Pan* VITEK® 2 Instrument User Manual 510731-10EN1 Carousel Temp.
TROUBLESHOOTING THE VITEK® 2 INSTRUMENT 8 Introduction This chapter helps you to resolve any problems that may occur while operating the VITEK® 2 instrument. The first section, Error Handling System, explains how the instrument reacts to an error, and how you, the user, should respond. The second section, Status Screen Error Conditions, covers the Instrument Halted screen, Message Status screen, and Error Status screen.
Troubleshooting the VITEK® 2 Instrument Diagnostic Tests • 8-19 Cleaning Optics (Cards Processing) • 8-23 Optical Diagnostic Test • 8-26 Boat Transport Positions • 8-26 Power Failures • 8-28 Displaying Version Information • 8-28 Using Error Message and Recovery Table • 8-29 Restarting the Instrument • 8-29 8-2 VITEK® 2 Instrument User Manual 510731-10EN1
Troubleshooting the VITEK® 2 Instrument Error Handling System Error Handling System The VITEK® 2 instrument continually monitors itself to ensure that it is operating within specifications. To do this it uses numerous optical, mechanical, and temperature sensors, located within each subsystem. If a sensor detects a problem, the VITEK® 2 instrument is programmed to alert you to the situation, and to provide you with the information you need to resolve the problem.
Status Screen Error Conditions Troubleshooting the VITEK® 2 Instrument • Look up the error code from the message in this chapter and follow the recovery procedure. Error Alarms VITEK® 2 has two alarms to alert you to the presence of an error condition. The dual alarms are provided in case the VITEK® 2 instrument and the workstation are in different locations. When they are in close proximity, the audible alarm on the instrument can be disabled as a configuration option. Audible Alarm.
Troubleshooting the VITEK® 2 Instrument Status Screen Error Conditions • Status screen with a status of Messages. Indicates there are error messages that need to be reviewed. These are minor errors that do not cause the Reader or Card Transport to halt. There are two ways you can handle a Status screen with a status of Messages. • Review the message and resolve the condition as indicated in Table 8-2. • Review the message and decide not to resolve the condition.
Troubleshooting the VITEK® 2 Instrument Status Screen Error Conditions 6 – 05:16. Time error occurred. Reviewing an Instrument Halted Screen 1) Note: Press Help (?) to view the error/message queue. If there is more than one message, use the Down Arrow to review and address each message. 2) Note the error code number, primary error code, and secondary error code listed on the error/message queue.
Troubleshooting the VITEK® 2 Instrument Status Screen Error Conditions 1 Figure 8-3: Transport Halted Screen (VITEK® 2) 1 Figure 8-4: Transport Halted Screen (VITEK® 2 XL) 4) Note: View the Transport Halted screen to find the jammed boat (1, Figure 8-3 or Figure 8-4 ). Jammed boats are indicated by dotted line rectangles. Non-jammed boats are indicated by solid line rectangles.
Troubleshooting the VITEK® 2 Instrument Status Screen Error Conditions indicate a Card Transport System Failure error again after the system has rebooted. 6) Close the front access door and the cassette load/unload door. LASER WARNING All access doors and covers must remain closed when processing cards to avoid exposure to laser light. 7) Press Go to exit the Transport Halted screen. 8) The instrument will make a clicking noise and automatically reboot.
Troubleshooting the VITEK® 2 Instrument Status Screen Error Conditions 2 3 4 5 6 Figure 8-6: Message Queue 2 — Primary Error Code. The first line in the error/message queue gives you the Primary Error Code (in this case, Hardware Error). For examples of other Primary Error Codes, see Table 8-2. 3 — Secondary Error Code. The second line of the error/message queue tells you the Secondary Error Code (in this case, Optical Controller Failure). For examples of other Secondary Error Codes, see Table 8-2.
Troubleshooting the VITEK® 2 Instrument Cassette Load Processing Errors • If the recovery procedure was successful, the Status screen appears with a Status of OK. • If the Instrument Halted screen reappears, record the error code number and call bioMérieux. • If a problem still exists, the word Errors will be displayed in the status field. The word Messages will display if a new message has been generated. If a problem recurs, contact bioMérieux.
Troubleshooting the VITEK® 2 Instrument Cassette Load Processing Errors • The bar code reader was unable to read one or more bar codes on the test cards. • The card has passed its expiration date. • An inconsistency was found between the information read from the button memory on the cassette and the bar codes on the test cards. For example, the button memory told VITEK® 2 that a Gram negative susceptibility card is in slot 4 of the cassette. The bar code reader, however, found that slot to be empty.
Cassette Load Processing Errors Troubleshooting the VITEK® 2 Instrument Figure 8-7: Bar Code Error Message 2) Open the door to the Cassette Load Station and remove the cassette. The door should remain open while resolving the bar code error. BIOHAZARD WARNING The cassette should be considered as potentially contaminated and should be handled appropriately. Qualified laboratory personnel should take the usual precautions necessary for infectious agents.
Troubleshooting the VITEK® 2 Instrument Note: Cassette Load Processing Errors The bar code you enter may be longer than the space provided on the screen. When this occurs, the first portion of the bar code scrolls off the display and the symbol > appears. 7) VITEK® 2 checks your entry for: • A valid bar code number. • An expiration date. • A card type match based on the bar code entered compared with the information read from the button memory on the cassette (Smart Carrier Mode only).
Cassette Load Processing Errors Troubleshooting the VITEK® 2 Instrument Figure 8-9: Card Type Error Message 2) Open the Cassette Load Station door, remove the cassette, and place it on the SCS. BIOHAZARD WARNING The cassette should be considered as potentially contaminated and should be handled appropriately. Qualified laboratory personnel should take the usual precautions necessary for infectious agents. 3) Press F3 to display the Summary screen.
Troubleshooting the VITEK® 2 Instrument Note: Cassette Load Processing Errors A matching identification card is one having the same accession ID and test type (gram +/gram -) as the susceptibility card. During the bar code read, the instrument checks for one of these two conditions. If the instrument detects an inoculum error: • The cassette is backed out to the Cassette Load Station. • The error alarm is activated.
Troubleshooting the VITEK® 2 Instrument Cassette Load Processing Errors Card Capacity Errors As the bar codes on the test cards are read, the cards are counted. The count is compared to the number of empty slots currently available in the reader carousel. If the number of cards exceeds the number of available slots: • The cassette is backed out to the Cassette Load Station. • The error alarm is activated. Resolving a Card Capacity Error 1) Press Help (?) to display the error/message queue.
Troubleshooting the VITEK® 2 Instrument Working Without the Bar Code Reader Disposables Errors When a cassette begins processing, VITEK® 2 calculates the quantity of disposables that will be needed. If the calculated value exceeds the current level of a disposable: • The cassette is backed out to the Cassette Load Station. • The error alarm is activated.
Troubleshooting the VITEK® 2 Instrument Instrument Diagnostics Main Menu > Utilities > Configuration The Bar Code Reader screen appears: Figure 8-12: Bar Code Reader Screen 2) Press Down Arrow to disable the bar code reader. Entering Bar Codes Manually 3) Access the Resolve Bar Code Errors option on the Main Menu. 4) Using the numeric keypad, enter the bar code from the bar code label. 5) Press Enter to accept the entry. Repeat this step for each test card in the cassette.
Troubleshooting the VITEK® 2 Instrument Instrument Diagnostics Diagnostic Tests Following are the tests found on the Diagnostics menu, and their function: Diagnostic Test Function Possible Results Temperature Reads the temperature in the reader carousel. Displays the carousel temperature in degrees Celsius. Also displays the word Low or High if the temperature is out of specification. Optics Performs a self-test of the transmittance optics systems.
Troubleshooting the VITEK® 2 Instrument Instrument Diagnostics If you have a VITEK® 2, the screen in Figure 8-14 displays. Figure 8-14: Temperature Diagnostics Screen (VITEK® 2) The screen displays the Incubator temperature and the temperature inside the instrument. Dispenser/Pipettor Diagnostic Test Note: The transport system must be idle to perform this test. This test is designed to be performed: • after installing an Accessory Kit to confirm correct operation of the dispenser/Pipettor Station.
Troubleshooting the VITEK® 2 Instrument Instrument Diagnostics • Look for repeated back and forth movement while the saline dispenser is in the upright position. The saline dispenser tube should move smoothly up and down when filling each AST tube. • Look for saline remaining in the saline dispenser tube when it returns to the upright position. • Look for a saline stream and/or drops released from the saline dispenser tube when it is filling with saline.
Troubleshooting the VITEK® 2 Instrument Instrument Diagnostics • Record the weight of the tube in slot 3 in the Pipettor High Volume section. Test tube 3 — Before test (B). • Record the weight of the tube in slot 5 in the Diluter section. Test tube 5 — Before test (B). Use Table 8-1 to record the weights of your test tubes. Make one copy for your use and keep the original in this manual.
Troubleshooting the VITEK® 2 Instrument Cleaning Optics (Cards Processing) 5) Open the cassette load door, load the test cassette and close the cassette load door. 6) Allow the cassette to process and return to the cassette load/unload door. Remove the cassette. 7) Record the weights of the test tubes placed in test tube slots 1, 3, and 5 using Table 8-1. • Record the weight of the tube in slot 1 in the Pipettor Low Volume section under Test tube 1 — After test (A).
Cleaning Optics (Cards Processing) Troubleshooting the VITEK® 2 Instrument CAUTION: Do not use commercial glass cleaner when cleaning the optics. This may result in calibration failures observed as the optics age. Clean optics using a quality, lint free lens paper that has been slightly moistened with alcohol.
Troubleshooting the VITEK® 2 Instrument Cleaning Optics (Cards Processing) The optics consist of multiple units that are hinged on the bottom and held in place by spring-loaded levers. Note: Do each step for all transmittance optic units at the same time. 6) Using your right hand, grasp the optics unit and place your thumb on the lever (2, Figure 8-16). 2 Figure 8-16: Opening the Optics Note: 7) Push down on the lever to release the unit and allow it to rotate on its hinges.
Troubleshooting the VITEK® 2 Instrument Boat Transport Positions 15) Continue with the Optical Diagnostic Test, noting the conditions of the test. Optical Diagnostic Test The Optical Diagnostic Test should not be performed if ID cards are processing. However, in some cases you need to perform the optical diagnostic test in response to an optics error.
Troubleshooting the VITEK® 2 Instrument Boat Transport Positions Halted Screen on page 8-6 for further information on card transport system jams. LASER WARNING All access doors and covers must remain closed when processing cards to avoid exposure to laser light. 4 5 6 1 3 2 Figure 8-17: Boat Transport Positions 1 – Cassette Load/Unload Station. Boats must not be removed or replaced at this station. This is the area you see when you open the front access door: 2 – Button Memory Station.
Troubleshooting the VITEK® 2 Instrument Power Failures the animated dotted lined rectangle and arrow on the Transport Halted screen. 4 – Vacuum Station. 5 – Cut/Seal Station. 6 – Card Load/Unload Station. Power Failures What happens in the VITEK® 2 instrument if there is a power failure? The instrument is supported by a device called an Uninterruptible Power Supply (UPS). If power is lost, the UPS uses a battery to continue to supply power to the instrument.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table A screen displays similar to the one shown in Figure 8-18. Figure 8-18: Version Information Screen Using Error Message and Recovery Table Table 8-2 in this section contains all of the messages that can be displayed in the error/message queue. The Error Message and Recovery table is designed to provide you, where possible, with the recovery procedure that is most likely to restore the instrument to normal operation.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table 2) Wait at least 10 seconds. 3) Move the AC power switch to the ON position. Table 8-2: Error Message and Recovery Table Error Code Recovery Procedure Primary Secondary Fatal Error Call bioMérieux Call bioMérieux for assistance. Hardware Error FLASH Memory Failure Call bioMérieux for assistance. Incubator Temperature Controller Failure Call bioMérieux for assistance. Internal Clock Call bioMérieux for assistance.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Initialization Error I Optical Error Secondary Card Sealer Failure Call bioMérieux for assistance. Card Transport System Failure Call bioMérieux for assistance. Dispenser Failure Call bioMérieux for assistance. Incubator Temperature Controller Failure Call bioMérieux for assistance.
Using Error Message and Recovery Table Troubleshooting the VITEK® 2 Instrument Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Error (Cont.) Secondary Carousel Jam 1) Open the waste collection door and top-right access door. 2) Remove the incubator access cover. (Refer to Step 5 of the procedure on page 7-12.) 3) Make sure that there is no card jammed between the reader head and the carousel. If there is, push the card into the carousel.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Error (Cont.) Secondary Dispenser Failure 1) Open the front access door and check for: • proper installation of the accessory kit. (See Accessory Kit installation procedure on page 7-4.) • a sufficient quantity of saline in the bag • tangles or pinches in the saline tubing 2) If needed, install a new accessory kit.
Using Error Message and Recovery Table Troubleshooting the VITEK® 2 Instrument Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Error (Cont.) Secondary Load Failure 1) If the instrument has not displayed the Halt screen, go to Step 6 of this recovery procedure. 2) If the Halt screen is displayed, open the waste collection door and top-right access door. 3) Remove the incubator access cover. (Refer to Step 5 of the procedure on page 7-12.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Error (Cont.) Secondary SCS information has errors 1) Remove the cassette from VITEK® 2. at Slot 2) Put the cassette on the SCS. 3) Press F10 to erase the data in the button memory. 4) Re-enter the data for the cassette. SCS memory contains old 1) Make sure that the VITEK® 2 instrument is in the correct cassette mode.
Using Error Message and Recovery Table Troubleshooting the VITEK® 2 Instrument Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Warning (Cont.) Secondary Cannot unload cards, 1) Open the Waste Collection Station door and check for jams. waste tray is not functional 2) Empty the waste collection tray. (See page 6-30). 3) Call bioMérieux for assistance if the problem recurs. Waste tray cannot hold the Empty the test card waste collection tray.
Troubleshooting the VITEK® 2 Instrument Using Error Message and Recovery Table Table 8-2: Error Message and Recovery Table (Continued) Error Code Recovery Procedure Primary Processing Warning (Cont.) Secondary Incubator Temperature LOW Ensure that the ambient room temperature is above 20 °C. Wait 15 minutes and recheck. If the incubator temperature has not returned to normal, call bioMérieux for assistance. Test cards that are still processing in the carousel will be terminated after this error occurs.
Using Error Message and Recovery Table 8-38 Troubleshooting the VITEK® 2 Instrument VITEK® 2 Instrument User Manual 510731-10EN1
APPENDIX A: HARDWARE SPECIFICATIONS A VITEK® 2 Instrument Dimensions Dimension VITEK® 2 VITEK® 2 XL Height 67 cm (26.3 in.) 67 cm (26.3 in.) Width 100 cm (39.4 in.) 140 cm (55.1 in.) Depth 71 cm (27.7 in.) 71 cm (27.7 in.) Mass VITEK® 2 Mass Approximately 110 kg (240 lb.) VITEK® 2 XL Approximately 145 kg (320 lb.) CAUTION: The VITEK® 2 instrument is heavy. Use at least two people to move it. Lift handles are provided at each end of the instrument.
VITEK® 2 Instrument Appendix A: Hardware Specifications Environment Note: The specifications below apply to both the VITEK® 2 and the VITEK® 2 XL.
VITEK® 2 Instrument Appendix A: Hardware Specifications Electrical Characteristics Electrical Characteristics Input voltage VITEK® 2 VITEK® 2 XL 200/240 VAC at 50/60 Hz 200/240 VAC at 50/60 Hz 100/120 VAC at 50/60 Hz 100/120 VAC at 50/60 Hz Maximum input current 4 amps @ 120 VAC or 2 amps @ 240 VAC 5 amps @ 120VAC or 2.8 amps @ 240 VAC Nominal input current 1.5 amps @ 120 VAC or 0.75 amps @ 240 VAC 2.75 amps @ 120 VAC or 1.
VITEK® 2 Instrument Appendix A: Hardware Specifications General Characteristics Note: The specifications below apply to both the VITEK® 2 and the VITEK® 2 XL. Cassette Capacity, cassettes: 4 cassettes maximum Capacity, cards: 15 cards per cassette Dispenser Dispensing volume: 2.33 – 2.
GLOSSARY Access Doors VITEK® 2 VITEK® 2 has six doors to enable the operator to access the internal systems and stations to replenish supplies and perform maintenance and cleaning procedures: top access door, saline access door, front access door, bottom access door, cassette load (and unload) door, and waste collection access door.
Glossary Bar Code An alphanumeric interface identifier label affixed to each VITEK® 2 test card. The bar code contains the card’s test type, lot number, and expiration date, which is entered either by the SCS subsystem by bar code wanding, or when the test card’s bar code label is read by the bar code reader inside of the VITEK® 2 instrument. Boat The tray-like device that carries the cassette around the instrument. It forms the bottom of the vacuum chamber and helps to capture any spills.
Glossary Dispenser/Pipettor Transfers and mixes the appropriate inoculum volume predetermined by the test card bar code (60 µL to 300µL - see Aliquot) to adjacent susceptibility tubes. The system includes a container for pipette tips, a displacement pump, and various tip mechanisms. Disposables VITEK® 2 disposables are pipette tips, sterile saline, test cards, and inoculum tubes. Disposable supplies are biohazardous after use and must be discarded according to local laws regarding biohazardous material.
Glossary SCS Base Unit See Smart Carrier Station (SCS). Sealer Station This station, a part of the Sample Preparation System, seals each test card by cutting and sealing its associated inoculum transfer tube with a hot wire. Smart Carrier Station (SCS) A laptop computer-like device used to aid in setting up the test and for entering test information. It consists of the base unit (computer) with LCD display, bar code scanner, and the keyboard (slides under base unit).
INDEX A Bar code scanner, SCS 2-7, 6-9, 6-16 Base pan, cleaning 7-28 Access doors 4-4 Base unit, SCS 2-9 Accession/Lab ID Field 6-18 Batch load 6-28 Accessories kit 7-4 installing 7-4–7-10 Batch loading 6-28 Address, cassette 6-21, 6-27 Boat 4-8 Alarm, audible 5-6, 8-4 enabling 5-11 setting volume of 5-12 Button memory cleaning 7-21–7-23 removing 7-21 removing from a cassette 7-19 Alarm, error 8-4 Alarm, visual 5-7, 8-4 enabling 5-11 Altitude acceptable operating range A-2 AST Card card, match
Index status screen indicator for 6-5 Cassette name defining 5-9 Dispenser station 4-11 on the status screen 6-5 selecting in cassette setup function 6-25 Dispenser/Pipettor Station 4-11 Displaying version info 8-28 Cassette setup function, using 6-24 Disposables 7-3–7-10 changing 7-4–7-10 load errors 8-17 monitoring 6-6, 7-3 Cassette worksheet 6-22 Caution, definition of 1-5 Changing pipette tips 7-8–7-10 saline 7-4 Documentation for VITEK® 2, overview 1-2 Doors, access 4-4 Character sets, definin
Index I Keypad, instrument 4-18 Icons, status screen 6-5 ID card L Language, SCS interface, setting 3-7 matching with AST 6-10 Indicator light, Cassette Loading Station 6-19 Light, indicator, Cassette Loading Station 6-19 Inoculum errors 8-14 Load errors, cassette 8-10 Instrument Loading a cassette 6-19–6-20, 6-25–6-27 bar code reader 5-6 card capacity 6-4 cleaning 7-24–7-25 connections 4-4 diagnostic tests 8-19 error handling system 8-3–8-10 external controls 4-4 QC Status 5-4 restarting 8-29 sc
Index Option boxes, SCS 6-14 Option buttons 6-9 Organism ID field 6-11 option box 6-14 P Pipette tips, changing See also Disposables 7-8 Pipettor station 4-12–4-13 Power Fail icon 6-6 Power failures 8-28 Previous screen key 6-9 Q configuration options, setting 3-3 configuration screens 3-2 data entry 6-17 data fields 6-11 function keys 6-13 keyboard 2-8 name 6-10 name, setting 3-3–3-4 option boxes 6-14–6-16 parts 2-6 processing cards with 6-16 slot indicator 6-12 starting 2-9 using 6-9 workflow descrip
Index QC 5-4 Status screen 6-3, 7-3 icons 6-5 in an error condition 8-4 with messages 8-8 System Requirements 2-9 T W Warning definition of 1-5 general warnings -i Waste collection station 4-17 Waste removal 6-30 Waste Tray Warning Level 5-8 Temperature checking instrument 8-19 operating range A-2 shipping and storing the instrument A-2 Test cards See also the VITEK® 2 Product Information.
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