Manual

ManualsBrandsWaters ManualsWater equipmentAccQ-Fluor Reagent Kit

[ Care and Use ManUal ]

AccQ•Fluor Reagent Kit 1

Contents

I. IntroduCtIon

II. Waters accQ•tag Method overvIeW

III. reagent PurIty

Iv. storage

v. reconstItutIng accQ•Fluor reagent

vI. usIng Waters accQ•Fluor reagent

vII. derIvatIzIng the calIbratIon standard

vIII. reconstItutIng the hydrolyzed saMPle

IX. derIvatIzIng the saMPle

X. storIng derIvatIves

XI. sPecIFIcatIons

XII. technIcal lIterature

XIII. orderIng InForMatIon

ACCQ•Fluor reagent kIt

I. IntroduCtIon

The Waters AccQ•Fluor™ Reagent Kit contains five sets of the deriva-

tizing reagents used to perform the AccQ•Tag™ Amino Acid Analysis

Method. A set consists of one vial each of:

– AccQ•Fluor Borate Buffer - Vial 1. You add the buffer to the sam-

ples to generate the proper pH environment for the derivatizatlon.

– AccQ•Fluor Reagent Powder - Vial 2A. The reagent powder Is the

6-aminoquinolyl-N-hydroxysuccinimidyI carbamate (AQC) derivatiz-

ing reagent. It Is shipped dry for maximum stability.

– AccQ•Fluor Reagent Diluent - Vial 2B. Use the diluent(acetonitrile)

to reconstitute the reagent for derivatization.

The Waters AccQ•Fluor Reagent Kit (Figure 1) contains sufficient

reagent for up to 250 analyses.

Figure 1: AccQ•Fluor Reagent Kit

Summary of content (5 pages)

PAGE 1
[ Care and Use Manual ] AccQ•Fluor reagent kit i. Introduction Contents The Waters AccQ•Fluor™ Reagent Kit contains five sets of the deriva- I. Introduction tizing reagents used to perform the AccQ•Tag™ Amino Acid Analysis Method. A set consists of one vial each of: II. Waters AccQ•Tag Method Overview – AccQ•Fluor Borate Buffer - Vial 1. You add the buffer to the sam- III. Reagent Purity ples to generate the proper pH environment for the derivatizatlon. IV.
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[ Care and Use Manual ] II. Waters AccQ•Tag Method overview 6. Heat Vial 2A on top of heating block, vortex occasionally, until the powder dissolves. Do not heat longer than 10 minutes. The AccQ•Tag Method is a precolumn derivatization and analysis Reconstituted AccQ•Fluor Reagent is approximately 10 mM technique for peptide and protein hydrolysate amino acids. To AQC in acetontrile. perform the AccQ-Tag Method: 1.
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[ Care and Use Manual ] VI. Using Waters AccQ•Fluor Reagent Derivitizing a Sample Note: The Waters AccQ•Fluor Reagent Kit is not for In vitro diagnostic Before derivatizing a vacuum-dried sample, you must reconstitute it. use. The Waters AccQ•Fluor Reagent Kit is for research use only. Use reconstituted AccQ•Fluor Reagent to convert samples and standards to highly stable fluorescent VIII. Reconstituting the hydrolyzed sample derivatives.
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[ Care and Use Manual ] XI. Specifications XIII. Ordering Information Waters manufactures the AccQ•Fluor Reagent Kit to exacting analyti- Item Part Number AccQ•Fluor Reagent Kit WAT052880 AccQ•Tag Chemistry Package WAT052875 AccQ•Tag Column, 3.9 x 150 mm WAT052885 AccQ•Tag Eluent A Concentrate, 950 ml WAT052890 AccQ•Tag EIuent B, 950 ml WAT052895 Ensure all glassware is clean.
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[ Care and Use Manual ] Sales Offices: Japan (81) 3 3471 7191 Austria and European Export (Central South Eastern Europe, CIS and Middle East) 431 877 18 07 Korea (82) 2 820 2700 Australia 2 9933 1777 The Netherlands +31 (0)76-50 87 200 Belgium 32 2 726 1000 Norway 47 63 84 60 50 Brazil 55 11 5094 3788 Poland (48) 22 833 4400 Canada 800 252 4752 Puerto Rico 787 747 8445 China 8621 6495 6999 Singapore 65 6273 1221 CIS/Russia +7 495 3367000 Spain 34 93 600 93 00 Czech Republic 42 02 617 11384