User Manual

ManualsBrandsWaters ManualsWater equipmentACQUITY UPC2 BEH, HSS, and CSH Columns

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. GETTING STARTED

a. Safety Considerations

b. Column Connectors and Installation

c. eCord Installation

d. Column Equilibration

e. Initial Column Efficiency Determination

f. VanGuard Pre-Columns

III. COLUMN USE

a. Sample Preparation

b. Solvents

c. Pressure

d. Useable Flow-Rate Ranges and Expected ACQUITY UPC

System Pressures

e. Band Spreading Minimization

IV. TROUBLESHOOTING

V. COLUMN CLEANING, AGING, REGENERATION,

AND STORAGE

a. Cleaning

b. Aging

c. Regeneration

d. Storage

VI. INTRODUCING ECORD INTELLIGENT

CHIP TECHNOLOGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

VII. ACQUITY UPC

COLUMN FAMILY

ACQUITY UPC

BEH, CSH, and HSS Columns

I. INTRODUCTION

Thank you for choosing an ACQUITY UPC

Column. The ACQUITY

UPC

Columns feature Ethylene-Bridged Hybrid (BEH), Charged-

Surface Hybrid (CSH™) and High-Strength Silica (HSS) particle

technologies to provide a wide range of selectivities, excellent peak

shape, and high efficiency. The ACQUITY UPC

packing materials

are designed for use with the ACQUITY UPC

Systems and are

manufactured in an ISO 9000 certified plant using ultra-pure

reagents. Each batch of ACQUITY UPC

material is tested and the

results are held to narrow specification ranges to assure excellent,

reproducible performance. Every column is individually tested and

a Performance Chromatogram and Certificate of Batch Analysis is

provided on the eCord™ Intelligent Chip.

ACQUITY UPC

Columns are designed and tested specifically for

use on ACQUITY UPC

Systems. ACQUITY UPC

Columns will

exhibit maximum chromatographic performance and benefit

ONLY when used on holistically-designed ACQUITY UPC

Systems

since these systems and columns were created and designed to

operate together. For these reasons, Waters cannot support the

use of ACQUITY UPC

Columns on any system other than an

ACQUITY UPC

System.

Summary of content (8 pages)

PAGE 1
[ CARE AND USE MANUAL ] ACQUITY UPC 2 BEH, CSH, and HSS Columns CONT ENT S I. INT RODUC T ION II. G E T T ING STA RT E D a. Safety Considerations b. Column Connectors and Installation c. eCord Installation d. Column Equilibration e. Initial Column Efficiency Determination f. VanGuard Pre-Columns III. COLUMN US E a. Sample Preparation b. Solvents c. Pressure d. Useable Flow-Rate Ranges and Expected ACQUITY UPC 2 System Pressures e. Band Spreading Minimization IV. T ROUBL E SHOOT ING V.
PAGE 2
[ CARE AND USE MANUAL ] Chemistry Particle Shape Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Pore Volume Carbon (cc/g) Load (%) Endcapped Particle Technology BEH 2-Ethylpyridine (2-EP) Spherical 1.7, 3.5 130 185 0.7 9 No BEH BEH (Unbonded) Spherical 1.7, 3.5 130 185 0.7 N/A N/A BEH CSH Fluoro-Phenyl Spherical 1.7, 3.5 130 185 0.7 10 No CSH HSS C18 SB Spherical 1.8, 3.5 100 230 0.7 8 No HSS Table 1. Physical Characteristics. II.
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[ CARE AND USE MANUAL ] 2. Determine the number of theoretical plates (N) and use this value as a benchmark for periodic comparisons. 3. Repeat the test at predetermined intervals to track column performance over time. (Slight variations may occur if performance tests are performed on two different ACQUITY UPC 2 Systems due to the quality of the connections, operating environment, system electronics, reagent quality and column condition.
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[ CARE AND USE MANUAL ] c. Pressure III. COLUMN US E To ensure the continued high performance of ACQUITY UPC Columns, follow these guidelines: 2 a. Sample Preparation 1. Sample impurities often contribute to column contamination. Waters offers both solid-phase extraction (SPE) and supercritical fluid extraction (SFE) options. For SPE, use Oasis ® Solid-Phase Extraction Cartridges/Columns or Sep-Pak ® Cartridges of the appropriate chemistry to cleanup the sample before analysis.
PAGE 5
[ CARE AND USE MANUAL ] ACQUITY UPC 2 Columns, 1.7 µm 100% CO2 60/40 CO2 /MeOH Dimension Predicted Max Flow Rate (mL/min) Predicted Max Pressure (psi) Predicted Max Flow Rate (mL/min) Predicted Max Pressure (psi) 2.1 x 50 mm 3.45 5581 2.00 6011 2.1 x 75 mm 2.65 5970 1.35 5938 2.1 x 100 mm 2.05 5969 1.05 6060 2.1 x 150 mm 1.40 5951 0.70 6002 3.0 x 50 mm 4.00 4179 3.60 5946 3.0 x 75 mm 3.75 4897 2.60 5959 3.0 x 100 mm 3.45 5499 2.00 5919 3.0 x 150 mm 2.70 5977 1.
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[ CARE AND USE MANUAL ] volumes of solvent. Increasing column temperature increases cleaning efficiency. If the column performance is poor after cleaning and regenerating, call your local Waters office for additional support. Note: Using an ACQUITY UPC2 System is not recommended for the cleaning and regeneration procedures because recommended solvents and high concentrations of water are immiscible with supercritical CO2.
PAGE 7
[ CARE AND USE MANUAL ] d. Storage Purging and storing a column in pure CO2 is recommended. This will eliminate or minimize retention changes of the column during storage. Storing the column in methanol is not recommended. eCord Fob V I. INT RODUC ING ECO RD INT E L LIG ENT C HI P T EC HNOLOGY a. Introduction The eCord Intelligent Chip will provide the history of a column’s performance throughout its lifetime.
PAGE 8
[ CARE AND USE MANUAL ] d. Column Use Information V II. ACQUIT Y U P C 2 COLUMN FAMILY The eCord Chip provides the customer with column use data, column dimensions and serial number. The overall column usage information includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum pressure and temperature.