User guide

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[ Care and Use ManUal ]

ACQUITY UPLC CSH Columns 1

Contents

I. GettInG started

a. Column Connectors

b. Column Installation

c. Column Equilibration

d. eCord Installation

e. Initial Column Efficiency Determination

f. VanGuard Pre-Columns

II. CoLUMn Use

a. Sample Preparation

b. pH Range

c. Solvents

d. Pressure

e. Temperature

III. CoLUMn CLeanInG, reGeneratInG and storaGe

a. Cleaning and Regeneration

b. Storage

IV. IntrodUCInG eCord InteLLIGent CHIP teCHnoLoGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

V. addItIonaL InForMatIon

a. Tips for Maximizing ACQUITY UPLC CSH Column Lifetimes

b. Recommended Flow Rates and Backpressures for

ACQUITY UPLC CSH Columns

ACQUITY UPLC CSH COLUMNS

Thank you for choosing a Waters ACQUITY UPLC

CSH

™

column.

ACQUITY UPLC CSH columns feature Waters Charged Surface

Hybrid (CSH) Technology which provides excellent peak shape,

high efficiency and loading capacity for basic compounds when

using acidic, low ionic strength mobile phases. This same particle

technology is used in the XSelect

™

CSH family of HPLC columns,

thus enabling seamless transferability between HPLC and UPLC

system platforms. The ACQUITY UPLC CSH packing materials were

designed specifically for use with the ACQUITY UPLC systems and

are manufactured in a cGMP, ISO 9001 certified manufacturing

facility using ultra pure reagents. Each batch of ACQUITY UPLC CSH

material is tested chromatographically with acidic, basic and neutral

analytes and the results are held to narrow specification ranges to

assure excellent, reproducible performance. Every column is individu-

ally tested and a Performance Chromatogram and Certificate of Batch

Analysis are provided on the eCord

™

intelligent chip.

ACQUITY UPLC CSH columns were designed and tested specifically

for use on ACQUITY UPLC systems. ACQUITY UPLC CSH columns will

exhibit maximum chromatographic performance and benefits ONLY

when used on holistically-designed ACQUITY UPLC systems since these

systems and columns were created and designed to operate together.

For these reasons, Waters cannot support the use of ACQUITY UPLC

columns on any system other than an ACQUITY UPLC system.

Summary of content (10 pages)

PAGE 1
[ Care and Use Manual ] ACQUITY UPLC CSH Columns Contents Thank you for choosing a Waters ACQUITY UPLC® CSH™ column. ACQUITY UPLC CSH columns feature Waters Charged Surface I. Getting started Hybrid (CSH) Technology which provides excellent peak shape, a. Column Connectors high efficiency and loading capacity for basic compounds when b. Column Installation using acidic, low ionic strength mobile phases. This same particle c.
PAGE 2
[ Care and Use Manual ] I. GETT ING STARTED c. Column Equilibration ACQUITY UPLC CSH columns are shipped in 100% acetonitrile. It is Each ACQUITY UPLC CSH column comes with a Certificate of important to ensure mobile-phase compatibility before changing to a Analysis and Performance Test Chromatogram embedded within different mobile-phase system. Equilibrate the column with a minimum the eCord intelligent chip.
PAGE 3
[ Care and Use Manual ] 2. Determine the number of theoretical plates (N) and use this value for 6. periodic comparisons. 3. Tighten with two 5/16” wrenches placed onto ACQUITY UPLC CSH column flats and VanGuard Pre-column hex nut (male end) as shown above. Repeat the test at predetermined intervals to track column performance over time. f. VanGuard Pre-Columns 7. Tighten 1/4 turn to set collet and ferrule. 8. Check that ferrule is set by loosening connection and inspecting the ferrule depth.
PAGE 4
[ Care and Use Manual ] A listing of commonly used buffers and additives is given in Table 3. Additionally, the column lifetime will vary depending upon the operating temperature, the type and concentration of buffer used. For example, the use of phosphate buffer at pH 8 in combination with elevated temperatures will lead to shorter column lifetimes.
PAGE 5
[ Care and Use Manual ] c. Solvents If the column performance is poor after regenerating and cleaning, call your local Waters office for additional support. To maintain maximum column performance, use high quality Table 4. Reversed-Phase Column Cleaning Sequence chromatography grade solvents. Filter all aqueous buffers prior to use through a 0.2 µm filter. Solvents containing suspended Polar Samples Non-polar Samples 1. water 1.
PAGE 6
[ Care and Use Manual ] IV. INTRODUCING eCORD INTELLIGENT C HIP TECHNOLOGY b. Installation Install the column into the column heater. Plug the eCord into the side of the column heater. Once the eCord is inserted into the column heater the a. Introduction identification and overall column usage information will be available The eCord intelligent chip will provide the history of a column’s allowing the user to access column information on their desktop. performance throughout its lifetime.
PAGE 7
[ Care and Use Manual ] d. Column Use Information The eCord chip provides the customer with column use data. The column dimensions and serial number. The overall column usage information includes the total number of samples, total number of injections, total sample sets, date of first injection, date of last injection, maximum pressure and temperature.
PAGE 8
[ Care and Use Manual ] V. ADDIT IONAL INFORMAT ION  a. Tips for Maximizing ACQUITY UPLC CSH Column Lifetimes 1.  5. attention to: 2. Water quality (including water purification system)  Solvent quality  Mobile-phase preparation, storage and age  Sample, buffer and mobile-phase solubilities  Sample quality and preparation. Investigate cause of column failure: When problems arise, often only one improper practice must 6.
PAGE 9
[ Care and Use Manual ] b. Recommended Flow Rates and Backpressures for ACQUITY UPLC CSH Columns 1.0 mm i.d. Columns (40 °C) UPLC Linear Velocity (mm/sec) Column Dimensions 1.0 x 50 mm 3 4 5 6 Flow Rate (mL/min) Backpressure (psi) Flow Rate (mL/min) Backpressure (psi) Flow Rate (mL/min) Backpressure (psi) Flow Rate (mL/min) Backpressure (psi) 0.1 4300 0.13 5600 0.17 7400 0.2 8700 1.0 x 100 mm 0.1 8600 0.13 11200 0.17 14600 0.2 17200 1.0 x 150 mm 0.1 12800 0.13 16700 0.
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[ Care and Use Manual ] Sales Offices Austria and European Export (Central South Eastern Europe, CIS and Middle East) 43 1 877 18 07 Australia 61 2 9933 1777 Belgium 32 2 726 1000 Brazil 55 11 4134 3788 Canada 1 800 252 4752 China 86 10 5293 6688 Czech Republic 420 2 617 11384 Denmark 45 46 59 8080 Finland 358 9 5659 6288 France 33 1 30 48 72 00 Germany 49 6196 400 600 Hong Kong 852 2964 1800 Hungary 36 1 350 5086 Norway 47 6 384 6050 Poland 48 22 833 4400 Puerto Rico 1 787 747 8445 Russia/CIS 7 495 727 4