User Manual

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[ Care and Use ManUal ]

NanoEase Trapping Columns 1

i. introduction:

Waters NanoEase Trapping Columns are robust devices that can effectively

separate complex samples and remove buffers and high concentrations of

salts. For the latest on Life Science Chemistries, go to:

www.waters.com/lifesciences.

ii. nanoEasE trap column prEparation

1. It is recommended that the trap column be washed and conditioned

with a suitable solvent before use. This can be accomplished using

a suitable syringe to flush solvent through the column. If a syringe

is used for this purpose, it is recommended that a syringe with an

inert teflon plunger and glass body be used to avoid contami-

nation. Syringes with rubber plungers or plastic bodies should

not be used. Alternatively, the trap column can be placed on the

HPLC system and washed with suitable solvent at a flow rate of

10-40 µL/min for several minutes prior to use.

2. If using a 10-port valve, use UpChurch

long nuts (UpChurch

part # F-285X) in each adjacent port of the 10-port valve, due to

the clearance width of the trap column.

3. The trap column works with the black 10-32 outer fitting with

white label (Figures 1a and 1b), which secures the stainless steel

tube and the tan PEEK

(column insert part) piece (Figure 2) to the

switching valve.

contEnts

i. introduction

ii. nanoEasE trap column prEparation

iii. GEnEral GuidElinEs for column usE

iv. ordErinG information

nanoEasE trappinG columns

Summary of content (4 pages)

PAGE 1
[ Care and Use Manual ] Nanoease trapping columns i. Introduction: Contents Waters NanoEase Trapping Columns are robust devices that can effectively separate complex samples and remove buffers and high concentrations of salts. For the latest on Life Science Chemistries, go to: I. introduction iI. NanoEase Trap Column Preparation www.waters.com/lifesciences. Iii. General Guidelines for Column Use iI. NanoEase Trap Column Preparation 1. iv.
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[ Care and Use Manual ] 4. To secure the column into a multi-port valve, care must be taken b) Screw in a column-blanking plug into the top of the black to get the face of the stainless steel barrel seated properly into fitting (Figure 3) until the stainless steel barrel protrudes the port without introducing dead volume between the frit at the from the end of the fitting approximately between 2 and 3 end of the stainless steel barrel and the valve port. The stainless mm (Figure C).
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[ Care and Use Manual ] c) IIi. General Guidelines for Column Use Grip the outside of black fitting (Figure 1a and 1b) and carefully screw it into the valve port to seal the trap into the Typical Separation Flow Rates: 1-40 µL/min valve, while being careful not to rotate the column-blanking Typical Loading Flow Rate: 15 µL/min for 4 minutes (with Waters CapL® and Stream Select Module) plug.
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[ Care and Use Manual ] iv. ordering information Description Particle Size Dimension Part No. Symmetry300 C18 Trap Column 5/pack 5 μm 0.18 x 23.5 mm 186002622 Atlantis dC18 Trap Column 5/pack 5 μm 0.18 x 23.5 mm 186002574 SCX 300 Trap Column 5/pack 5 μm 0.50 x 23.5 mm 186002623 ™ ® ® Symmetry C18 Trap Column 5/pack 5 μm 0.18 x 23.5 mm 186002808 0.18 i.d. Custom Trap Column 5/pack Custom 0.18 x 23.5 mm 186002687 0.50 i.d. Custom Trap Column 5/pack Custom 0.50 x 23.