User guide

Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
[ CARE AND USE MANUAL ]
©2014 Waters Corporation. Waters, The Science of What’s
Possible, Oasis, Sep-Pak, Empower, MassLynx, and XBridge
are registered trademarks of Waters Corporation. eCord is a
trademarks of Waters Corporation. All other trademarks are
property of their respective owners.
February 2014 715001465 Rev C VW-PDF
T1
T8
T15
T5
T19
T12-T13
T12
T4
T9-T10
T10
T14
T13-T14
T19C
Minutes
6.00 9.00 12.00 15.00 18.00 21.00 24.00
*Chromatographic Conditions:
Column: 2.1 mm x 100 mm,
Sample: Tryptic digest of cytochrome c;
Flow rate: 0.20 mL/min
Mobile phase A: 0.045% TFA in water;
Mobile phase B: 0.045% TFA in acetonitrile;
Gradient: from 0 to 15% B in 6 min, from 15 to
36% B in 20 min;
Temperature: 35 ˚C; Detection: UV 214 nm
VI. REPRESENTATIVE TEST CHROMATOGRAPH AND CONDITIONS FOR SEPARATION/
OF PROTEIN DIGEST
Peak Identification
T1 N-AcGDVEK T8 TGPNLHGLFGR
T13-T14 KYIPGTK T15 MIFAGIK
T14 YIPGTK T5 CAQCHTVEK (heme attached)
T4 IFVQK T19 EDLIAYLK
T9-T10 KTGQAPGFSYTDANK T12-T13 GITWGEETLMEYLENPKK
T10 TGQAPGFSYTDANK T12 GITWGEETLMEYLENPK
T19C EDLIAY