Manual

[ Care and Use ManUal ]
PoraPak
™
RXN Cartridges and Bulk Care & Use 2
I. Common ApplICAtIons for CleAnup
Bases
Acids
Neutrals
Catch and Elute
Pass Through
Adsorption
PoraPak Rxn CX
PoraPak Rxn CX
PoraPak Rxn RP
TFA removal
Convert from high boiling
point solvent for bases
Catch and Elute
Catch and Elute
PoraPak Rxn CX
PoraPak Rxn CX
Fractionate sample
before Prep for bases
Remove water from post
column fraction for bases
Catch and Elute
Catch and Elute
PoraPak Rxn CX
PoraPak Rxn CX
Technique Recommended Product
Reaction Products
Reaction Components
Pre-and-Post
Preparative Column
Catch and Elute
Inthe“catchandelute”technique,thereactionmixtureisloadedontothecartridge;theanalytesofinterestareretainedbythesorbent.Awashstepfollowstoremove
additionalreactioncomponentsfromthecartridge.Astrongsolventisusedtoelutetheanalytesfromthecartridge.Sampleconcentrationresultswhenthefinalelution
volume is smaller than the load volume.
Pass Through
Inthepass-throughtechnique,thesorbentdoesnotretainthecompoundsofinterest,butdoesretainsomeclass(es)ofcompoundsandimpurities,thatcanbediscarded
with the column. Fractionation is achieved by the interaction between the impurities in the reaction mixture and the sorbent. No sample enrichment occurs during the
solid-phaseextraction(SPE)step.
Adsorption
Intheadsorptionchromatographytechniqueonareversed-phasesorbent,theneutralcompoundsaremovingdownthechromatographiccolumninthepresenceof
organicsolvent,albeitslowly,forsmall-percentorganicwashes.Loadthereactionmixtureinhighwatercontent
(75%waterforexample),washtoremovepolarcompounds,andthenincreasetheorganiccontentinlargeincrementstoeluteneutrals.Theincreaseisdeterminedby
the hydrophobicity and MW of the compounds.