Manual

[ Care and Use ManUal ]
PoraPak
™
RXN Cartridges and Bulk Care & Use 4
III. produCt GuIdelInes
Thefollowingtablesshouldbeusedasguidelinesforloadingcapacity,flowrates,andvolumesofwashandelutionsolvents.Thesetablestakeintoconsiderationthe
dimensionofthedeviceandthebedvolumessuggestappropriateflowratesandvolumesforthecondition,washandelutesteps.
Thecapacityisforthetotalofallcompoundsinthereactionthatwillberetained.Forcationexchange,thecapacityisforallbasesinthereactionmixtureincluding
product and reactants.
Consider smaller loads than maximum capacity guidelines or choose a larger device to avoid compound break through.
Flow rate guideline is meant to provide a flow for reaction loading step.
Thereactionmixcanbepouredintothecartridgeandallowedtoflowbygravity.Iftheflowistooslow,simplyaddasmallamountofpressureorvacuumtoincreaseit.
Ifthereisprecipitateinthereactionvessel,rinsethevesselwithmoreloadingsolventandaddtothetopofthecartridge.Duringthewashingsteps,producttrappedby
the precipitate may be dissolved and can be retained on the packed bed for later elution under the right solvent conditions.
Thistableprovidesguidelinesforvolumesusedinthecondition,washandelutionstepsforthevarioussizesofPoraPakRxncartridges.
Maximum Capacity Guidelines PoraPak Rxn RP PoraPak Rxn CX
Configuration Retained Compounds Retained Base
6cc-150mg 15mg/50µmole 15mg/50µmole
6cc-400mg 40mg/150µmole 40mg/150µmole
20cc-2g 200mg/350µmole 200mg/350µmole
60cc-5g 500mg/2000µmole 500mg/2000µmole
Wash and Elution Volumes (mL)
Device Condition Wash Elute
6cc-150mg 3mL 4mL 4mL
6cc-400mg 5mL 10mL 10mL
20cc-2g 20mL 20mL 20mL
60cc-5g 45mL 45mL 45mL
Flow Rate Guidelines (mL/min)
6 cc cartridge 2mL/min
20cccartridge 5mL/min
60cccartridge 9mL/min