User's Manual

11
Equipment and reagents for Xdrop™ enrichment and amplification supplied by the user
In addition to required and suggested Samplix products, the following items are required.
Equipment
Cell sorter instrument
Real-time PCR cycler
Thermal cycler with slow ramping function
Instrument for nucleic acid quantification e.g. Qubit™, Bioanalyzer™, TapeStation™, FEMTO Pulse™ or
similar.
LAF (Laminar Air Flow) hood
Microcentrifuge
Vortex
Pipette set from P2 to P1000
Freezing blocks for both PCR tubes and microcentrifuge tubes
Consumables
Nuclease free PCR tubes and microcentrifuge tubes
Eppendorf DNA LoBind tubes 1,5 ml tubes (Catalog No. 0030108051)
Filtered pipette tips
Wide bore pipette tips for P200 pipette outer diameter of tip: 1 mm – 1,9 mm
Reagents
Nuclease-free water