User's Manual

17
Optional: Positive control dPCR reaction (Cat. No. CO10200)
Note that it is possible to include the Positive control kit (Cat. No. CO10200) provided by Samplix into the
dPCR reaction. The kit includes both Positive control DNA ● and dPCR control primers ● (already mixed) with
an annealing temperature of
60°C. See table below.
dPCR reaction mix
1 reaction
H
2
O (molecular grade)
16,4 μl
dPCR mix (2x) ●
20 μl
dPCR control primers
●
mix
1,6 μl
Total mix
38 μl
Positive control DNA ●
2 μl
Total volume
40 μl
Validation primers ● (already mixed) are also provided within the kit to perform the enrichment validation
(see Chapter 6).
Prepare dPCR cartridge
1. Dilute the 2x dPCR buffer ● to 1x with molecular grade water. Note: Dilute an entire vial of dPCR
buffer. Precipitates potentially present in the 2x buffer will dissolve when diluted to 1x. Make sure to
mix well by vortexing for 10 seconds or inverting the tube >20 times. Use leftover 1x dPCR buffer for
sorting of droplets (chapter 4).
2. Unpack the cartridge from the plastic bag and place it on a clean flat surface in a LAF (Laminar Air
Flow) hood or a similar clean, dust free environment. The layout of the cartridge is depicted in Fig.
3.1.
3. Handle the cartridge as follows:
• Always use gloves when handling the cartridge.
• Hold the cartridge by its sides.
• Do not touch any of the input wells or droplet exit well.
• Do not touch the microfluidic chip on the back of the cartridge.
• Save the cartridge storage bag for later storage of the cartridge.