User's Manual

4. Be careful not to use the same lane more than once as this will disrupt droplet production. To avoid

using the same lane more than once mark the storage plastic bag or the cartridge directly with a

permanent marker once a lane has been used.

5. Load the cartridge with reagents in a LAF hood or a similar clean, dust free environment. Allow the

reaction mix to reach room temperature before loading on the dPCR cartridge followed by immediate

insertion on the Xdrop™ instrument.

Note: it is important to load the reagents on the cartridge in the order described on the next page.

Fig. 3.1. dPCR cartridge top view with wells (A-D) and lanes (1-8) marked.