User's Manual

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Fig. 3.2. Cross section of the dPCR cartridge. Notice the exact location of the shelf in well #D, where 40 μ
l
of 1x dPCR buffer ● are added.
When loading the dPCR cartridge, avoid introducing air bubbles by pipetting on the side wall of the wells.
6. Load 300 μl 1x dPCR buffer ● in the first well (#A). It is important to load the cartridge in the order
described here and avoid air bubbles by pipetting carefully on the side of the well.
7. Load 40 μl 1x dPCR buffer ● to the shelf of the collection well #D (Fig. 3.2).
8. Load 40 μl dPCR reaction mix including primers and sample DNA into well #C.
9. Load 100 μl dPCR oil ● into well #B.
Notice that the order of loading is: 300 μl 1x dPCR buffer ● in the well #A
40 μl 1x dPCR buffer ● to the shelf #D
40 μl 1x dPCR reaction mix in well #C
100 μl dPCR oil ● in well #B
Well #D shelf