User's Manual

2
Table of Contents
Chapter 1: Xdrop
TM
at a Glance page 3
Targeted enrichment overview
Workflow overview
Xdrop™ instrument overview
Required items for Xdrop™
Suggested Samplix products
Equipment and reagents for Xdrop™ supplied by the user
Chapter 2: Droplet PCR Reaction Design for Target Selection page 12
Target sequence
Primer design guidelines
Primer Testing
Chapter 3: Droplet PCR (dPCR) page 16
Preparations for dPCR
Setup of dPCR reaction
Optional: Positive control dPCR reaction
Prepare dPCR cartridge
Collect generated droplets
Chapter 4: Single DNA Molecule Detection and Sorting of Droplets page 26
Requirements for cell sorter
Notes to operator
Preparation of droplets for flow cytometry
Flow cytometry analysis and sorting of dPCR droplets
Optional: Set up flow cytometry with Cell sorter control droplets
Chapter 5: Multiple Displacement Amplification in droplets (dMDA) page 31
Break sorted droplets (if applicable)
Set up dMDA reaction
Load the dMDA cartridge
Generate dMDA droplets on the Xdrop™ instrument
Chapter 6: Evaluation of Amplification and Target Enrichment page 42
Quantify total DNA
Evaluate the enrichment of target DNA
Calculate fold enrichment of target DNA
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