User's Manual

3
Chapter 1: Xdrop™ at a Glance
Targeted enrichment overview
Congratulations with your new Xdrop™ instrument, which we expect will facilitate groundbreaking research.
The Xdrop™ introduces a new approach for genomic analysis. Our innovative technology enables targeted
enrichment of genomic regions in droplets. The Xdrop™ system offers sensitive and unbiased PCR-free
sample enrichment and isothermal amplification prior to downstream analysis e.g. next generation
sequencing.
Using the Xdrop™ instrument, cartridges, and reagents, DNA samples are partitioned into millions of picolitre
size droplets. The workflow includes two steps:
1. Target enrichment in double emulsion droplets (Chapter 3, Droplet PCR – dPCR)
2. Isothermal amplification in single emulsion droplets (Chapter 5, Multiple Displacement Amplification
in droplets – dMDA)
Fig. 1.1. Xdrop™ workflow including dPCR (droplet PCR) and dMDA (Multiple Displacement Amplification in
droplets).