User's Manual

33
Load the dMDA cartridge
The dMDA reaction takes place inside droplets formed in the dMDA cartridge inserted in the holder (Fig. 5.2).
The cartridge must be sealed with a gasket on top during droplet production. Load samples in a clean LAF
hood.
Note: The dMDA holder should be re-used for all the following runs.
Fig. 5.2. Left: dMDA holder. Right: Top view of dMDA cartridge with inlet well on top and collection well
below. Bottom: zoomed view of the holes of the inlet well.
Set up the cartridge:
1. Wearing gloves, unpack the dMDA holder and cartridge from their plastic bag.
2. Handle the cartridge as follows:
• Always wear gloves when handling the cartridge.
• Hold the cartridge by its sides or by the handle.
• Do not touch any of the inlet wells or droplet collection wells.
dMDA holder
dMDA cartridge
Inlet well
Collection well
Catridge
handle
dMDA oil
Sample
mix