User's Manual

35
Fig. 5.4. Injection of the sample in the inlet well. From left to right: Cartridge inlet where to load the sample
mix: the wide bore pipette tip should enter the hole in the pointy end of the tear shaped inlet well. Correct
positioning of the wide bore pipette tip. Incorrect positioning of the wide bore pipette tip. Outer diameter
of the wide bore pipette tip (1-1,9 mm). Picture of dMDA cartridge loading of the sample mix.
Fig. 5.5. Loading of the dMDA cartridge. 1) Collect your sample mix. 2) Place the wide bore pipette tip in
the hole at the pointed end of the tear shaped inlet well. 3) Load the sample by slowly pressing the
plunger to the first stop and keep it pressed for 15 seconds; this allows the mix to enter the microfluidic
system. 4) Keep the plunger down at the first stop when removing the tip from the cartridge.
6. Slowly inject the sample and hold the pipette plunger in the first stop position for 15 seconds while
still applying moderate pressure on the pipette tip to ensure a tight connection between the pipette
and cartridge (see Fig. 5.4 and Fig. 5.5).
7. Remove the pipette while still holding the plunger button in the first position. The entire sample
should have entered the channel in the chip, there should be no liquid visible in the well (see Fig. 5.5).
8. Repeat steps 5 to 7 for the next lane to use, if applicable.
Inject and hold on
1
st
stop 15 sec
Hold on 1
st
stop
when removing
from cartridge
Collect your
sample mix
Place tip in the
inlet well
1
2
3
4
Load sample mix here
Outer diameter 1- 1,9 mm