User's Manual

Fig. 6.2 Example of amplification and standard curves for qPCR QC validation of enrichment. Left: In the

Amplification plot, the curve in red represents the dMDA amplified sorted positive population, while the grey

shades represent 10x, 1x and 0,1x reference DNA (from darker to lighter). Right: Standard curve and

calculations of PCR efficiency.

Calculate fold enrichment of target DNA

Use the “Enrichment calculator” in the “Tool” section under “Resources” on the Samplix homepage (Fig. 6.3)

to calculate the fold enrichment of target DNA available at samplix.com

Fig. 6.3. Choose one of the two “Enrichment calculator” tools shown on the right. Enrichment can be

calculated based on the number of targets per genome or the number of targets per nanogram of input

DNA.