Functional description photoLab® 6100 VIS Spectrophotometer ba75847e01 08/2009 (identical to spectroFlex 6100)
photoLab® 6100 VIS Accuracy when going to press The use of advanced technology and the high quality standard of our instruments are the result of continuous development. This may result in differences between this operating manual and your instrument. Also, we cannot guarantee that there are absolutely no errors in this manual. Therefore, we are sure you will understand that we cannot accept any legal claims resulting from the data, figures or descriptions.
photoLab® 6100 VIS Contents photoLab® 6100 VIS - Contents 1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.1 1.2 1.3 2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.1 2.2 2.3 2.4 3 3.3 4.3 08/2009 11 12 12 13 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2.1 Inserting the buffer batteries . . . . . . . . . . .
photoLab® 6100 VIS Contents 4.4 4.5 Zero adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Measuring in Concentration mode . . . . . . . . . . . . . . . . . 41 4.5.1 Measuring cell tests with barcode . . . . . . . . . . . 41 4.5.2 Measuring reagent tests with AutoSelector . . . . 42 4.5.3 Measuring reagent-free tests and user-defined methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 4.5.4 Exceeding the upper or lower limits of the measuring range . . . . .
photoLab® 6100 VIS Contents 4.10.2 Analysis timer . . . . . . . . . . . . . . . . . . . . . . . . . 4.11 Memory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.11.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.11.2 Instructions on using USB memory devices 4.11.3 Measurement datasets . . . . . . . . . . . . . . . . . . 4.11.4 Saving measurement datasets manually . . . . 4.11.5 Saving measurement datasets automatically . 4.11.
photoLab® 6100 VIS Contents 5 Maintenance and cleaning . . . . . . . . . . . . . . . . . . . . . 151 5.1 5.2 5.3 6 What to do if ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 6.1 6.2 7 Actions in the case of a broken cell . . . . . . . . . . . . . . . 155 Error causes and remedies . . . . . . . . . . . . . . . . . . . . . . 156 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 7.1 7.2 7.3 8 Replacing the lamp . . . . . . . . . . . . . . . . .
photoLab® 6100 VIS Overview 1 Overview 1.1 Overview of the instrument Front of the instrument 3 4 5 6 1 2 1 2 3 4 5 6 Fig. 1-1 Socket field on the rear panel Front of the instrument with operating elements 7 8 9 10 7 8 9 10 Fig. 1-2 Display Keypad Shaft for rectangular cells Turn-up lid Shaft for round cells Cell shaft cover Connection for power pack RS232 connection USB-A connection USB-B connection Rear panel with socket field Note All connections comply with SELV.
photoLab® 6100 VIS Overview 1.2 Keypad Overview 1 2 1 2 3 Function keys F1 to F4 (function menu-depending) Alphanumeric keypad Keys with dedicated function Fig. 1-3 Key functions 3 Keypad The keys on the right side of the keypad have the following functions: Key Designation Functions – Switches on and off the photometer. – Switches to the main menu from any operating situation. Actions that are not completed are canceled.
photoLab® 6100 VIS Overview Key Designation Functions – Opens the menu, Timer. – Cancels the running action. Entries that have not yet been accepted are discarded. – Switches to the next higher menu level. – Starts an action (e.g.
photoLab® 6100 VIS Overview 1.3 Display elements Display Concentration 1.92 18: 14752 1 2 3 10 mg/l 2 0.05 - 3.00 mg/l Method list Citation form Unit 3 Status line (current state, date and time) Display range for menus and measurement results Function keys menu Bild 1-4 Symbols in the status line 1 NH4-N 10 mm Setup 04/16/07 9:52 Symbol Display Designation Function Save The key is active. You can store the displayed data with (see section 4.11).
photoLab® 6100 VIS Safety instructions 2 Safety instructions This operating manual contains basic instructions that you must follow during the commissioning, operation and maintenance of the photometer. Consequently, all responsible personnel must read this operating manual carefully before working with the meter. Keep this operating manual in the vicinity of the meter.
photoLab® 6100 VIS Safety instructions 2.2 Authorized use The authorized use of the photometer consists exclusively of the carrying out of photometric measurements according to this operating manual. Follow the technical specifications of the cells in chapter 7 TECHNICAL DATA. Any other use is considered to be unauthorized. 2.3 General safety instructions The photometer is built and inspected according to the relevant guidelines and norms for electronic instruments (see chapter 7 TECHNICAL DATA).
photoLab® 6100 VIS Safety instructions 2.4 Handling of hazardous substances When developing test sets, WTW carefully sees that the tests can be carried out as safely as possible. Some hazards by dangerous substances, however, cannot always be avoided. Warning Improper handling of certain reagents can cause damage to your health. In any case follow the safety labels on the packing and the safety instructions of the package insert. Protective measures specified there have to be followed exactly.
Safety instructions 14 photoLab® 6100 VIS ba75847e01 08/2009
photoLab® 6100 VIS Commissioning 3 Commissioning 3.1 Scope of delivery z Spectrophotometer photoLab® 6100 VIS z Power pack connection cable z Buffer batteries 4 x AA alkaline manganese (Mignon) z Zero cell (16 mm, round) z Short instructions z CD-ROM with – Detailed operating manual – Analysis instructions – SpectralTransfer software – Software update "Chinese character set" (see section 4.19.3) Packing This photometer is sent out in a protective transport packing.
photoLab® 6100 VIS Commissioning 3.2.1 Inserting the buffer batteries The buffer batteries supply the integrated clock while the photometer is switched off. Four alkaline manganese batteries (type AA or Mignon) separately included in the scope of delivery are used as the buffer batteries. Insert the batteries as follows: 1 Turn the photometer upside down and place it on a soft surface. 2 Open the lid of the battery compartment (1). 3 Insert the four batteries in the battery compartment.
photoLab® 6100 VIS Commissioning 3.2.2 Connecting the power supply The power is supplied with the aid of the enclosed plug-in power pack. The power pack supplies the photometer with low voltage (12 VDC). Caution The line voltage of the usage location must fulfill the specifications stated on the power pack (the specifications are also given in chapter 7 TECHNICAL DATA). Always use the supplied 12 V original power pack only.
photoLab® 6100 VIS Commissioning 3.2.3 Switching on the photometer for the first time During the initial commissioning, the photometer automatically guides you through the setting of the meter language, date and time after switching on (see following sections). Language 16.04.07 9:52 1 Deutsch ✓ Press . The photometer is switched on. English Français Español The display switches to the setting of the language (see section 3.2.4).
photoLab® 6100 VIS Commissioning 3.2.5 Setting the date and time During the initial commissioning, the instrument automatically guides you to the setting of the time and date after the setting of the language. Date/Time 16.04.07 9:52 Date Time 16.04.2007 9:52:09 The Date/Time menu is open. Using <▲><▼>, select a menu item and confirm or open it with . 1 Select and confirm Date. The input field for the current date pops up. OK Date/Time 16.04.07 9:52 Date Time 16.04.
photoLab® 6100 VIS Commissioning 3.3 Connecting optional accessories 3.3.1 Communication interfaces Connections RS232 Fig. 3-1 USB-A USB-B Communication interfaces on the rear panel You can connect the following accessories to the photometer: z PC (see section 3.3.2) z Printer (see section 3.3.2) z USB storage media (see section 3.3.3) z USB-PC keyboard (see section 3.3.4) z Barcode reader (see section 3.3.5) z Car Adapter photoLab® 6xxx (see section 3.3.
photoLab® 6100 VIS Commissioning 3.3.2 PC/printer PC and printer can be connected to the photometer as follows: Interface PC Printer RS232 Functions The data is sent to the interface with . z If a printer is connected the data is printed out. z If a PC is connected, the data can be received with a terminal program (see section 4.13). USB-A USB-B The data is printed out with . - Enables the direct connection of photometer and PC.
photoLab® 6100 VIS Commissioning Operation at RS232 Connect the RS232 interface to the devices as follows: z PC: with a commercially available zero modem cable z Printer: with a commercially available RS232 printer cable The cables are available in specialized computer shops. Set up the following interface data at the PC/printer: Baud rate Selectable from 1200, 2400, 4800, 9600, 19200 The baud rate must agree with the baud rate set on the PC/printer.
photoLab® 6100 VIS Commissioning The USB-PC keyboard is connected to the USB-A interface. 3.3.5 Barcode reader The barcode reader enables the simplified entering of alphanumerical character strings and can be used in all operating situations that require the entry of text or numerals. The barcode reader is connected to the USB-A interface. 3.3.
photoLab® 6100 VIS Commissioning Note The spectrophotometer consumes electricity even while it is in standby mode. We recommend to disconnect the photoLab® 6100 VIS if you do not use the spectrophotometer during battery operation. Technical data Car Adapter photoLab® 6xxx 24 Cable length 2m Max. voltage 12 V Max.
photoLab® 6100 VIS Operation 4 Operation 4.1 Switching on or off the photometer Switching on 1 Switch the photometer on with . Self test 04/16/07 9:52 The display shows – the Self test dialog (if the user management is not active). Please make sure no cell is inserted and the cover is closed. Then press Setup – the Login dialog (if the user management is active).
photoLab® 6100 VIS Operation Self test During the self-test, all cells must be removed and the cell shaft cover closed. Self test Keep cover closed System test Filter test Lamp test Wavelength calibration 04/16/07 9:52 The self-test includes: – the test of the memory, processor, internal interfaces, filter and lamp – a calibration for each wavelength After the self-test is completed, the main menu is displayed.
photoLab® 6100 VIS AutoCheck Operation With the AutoCheck function the photometer checks and calibrates the optical measuring unit. The AutoCheck is automatically carried out if measurement settings were changed since the last measurement, e.g.: z if a different wavelength was selected or z if a different method was selected. If necessary, the photometer asks you to remove the cell from the cell shaft.
photoLab® 6100 VIS Operation 4.2 General operating principles 4.2.
photoLab® 6100 VIS Use of the function keys Operation The function keys F1 to F4 are below the display. Their functions change depending on the operating situation and mode. The current functions are displayed in the function key menu at the bottom edge of the display.
photoLab® 6100 VIS Operation 4.2.2 Display of navigation paths in short form In this operating manual, the introductory navigation steps leading to individual menus or dialogs are clearly shown in a gray box. The box indicates a section of the menu tree. Starting point of the description is always the main menu, which can be reached with the key from any operating situation. From there navigation takes place downward.
photoLab® 6100 VIS Operation Note The complete menu tree is given in the appendix of this operating manual. 4.2.3 Entry of numerals, letters and characters Numerals, letters, punctuation marks and special characters are entered with the alphanumeric keypad of the meter or using an external keyboard. Entries are required in operating situations such as the following: z Entering the date and time z Entering an ID e.g.
photoLab® 6100 VIS Operation Note During mere number entries (such as entering a wavelength), the keys of the alphanumeric keypad are assigned to the respective numeral only. Each keypressing directly enters the numeral (like a pocket calculator). Special characters Operating example: Entering the ID Special characters are entered with the <1/*> key. The Enter ID input field appears if you press the key while the storing symbol is visible.
photoLab® 6100 VIS Operation 4.2.4 Detailed operating example: Changing the language 1 Call up the main menu with the key. 2 Open the General setup menu with the F1 function key [Setup]. General setup 04/16/07 9:52 Language Date/Time Display settings User managementg The Language menu shows a list with the available languages. The currently active language is marked by a tick.
photoLab® 6100 VIS Operation 4.3 Photometer settings and system administration The general photometer settings are done in the -> General setup menu. The general photometer settings comprise: z Language (see section 4.3.2) z Date/time (see section 4.3.2 and section 4.2.4) z Display characteristics (see section 4.3.3) z User management (see section 4.15) z Administration of the measurement data memory (see section 4.11) z Software and method update (see section 4.
photoLab® 6100 VIS Operation 4.3.2 Date/Time The date format is set automatically with the language setting. According to the locally usual version, the date format is displayed in the order, Day.Month.Year (DD.MM.YY) or Month/Day/Year (MM/DD/YY or MM.DD.YY). [General setup] – Date/Time The Date/Time menu is open. 1 Select and confirm Date. The input field for the current date pops up. Date/Time 04/16/07 9:52 Date Time 16.04.2007 9:52:09 2 Enter the current date with <0...9> and confirm.
photoLab® 6100 VIS Operation 4.3.3 Display settings Here you can adjust the display contrast to the lighting conditions. [General setup] – Display settings Display settings 04/16/07 9:52 Contrast 50 % 1 A slide control for the display contrast appears. 2 36 Select and confirm Contrast. Using , set the display contrast and confirm.
photoLab® 6100 VIS Operation 4.4 Zero adjustment A valid zero adjustment is required for the calculation of measured values in the modes, Concentration, Absorbance / % Transmission, Multi wavelengths and Kinetics. With a zero adjustment, the absorbance of a cell filled with distilled water ("zero cell") is measured and stored.
photoLab® 6100 VIS Operation Note Ordering information is given in chapter 8 ACCESSORIES AND OPTIONS. The cells listed in the chapter 8 ACCESSORIES AND OPTIONS are especially adapted to the WTW test set program. General requirements of the cells are given in chapter 7 TECHNICAL DATA. Note that the spectral transparency of the cell must be suitable for the intended application (example, quartz cell for UV range).
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Zero cell (H2O dist.) Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.
photoLab® 6100 VIS Operation Validity of the zero adjustment The data of the zero adjustment is stored in the photometer separately for each cell type. As long as the data is valid, it is automatically used again after a temporary change to a different cell type.
photoLab® 6100 VIS Operation 4.5 Measuring in Concentration mode 4.5.1 Measuring cell tests with barcode Concentration Concentration 04/16/07 9:52 Inserting a cell with barcode starts a measurement. Please select method for measuring or insert a barcoded cell or insert AutoSelector. Setup Method list Last method New Method 1 Open the cell shaft cover. Line mark Barcode 2 Close the inner turn-up lid.
photoLab® 6100 VIS Operation 4.5.2 Measuring reagent tests with AutoSelector Concentration Concentration The method is selected by inserting the AutoSelector. 04/16/07 9:52 Please select method for measuring or insert a barcoded cell or insert AutoSelector. Setup Method list Last method Concentration New Method The photometer is ready to measure. 04/16/07 9:52 To start measurement, insert cell or press 38: 14761 Fe 10 mm 0.05 - 5.
photoLab® 6100 VIS Operation 3 Open the inner turn-up lid. 4 Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The correct measuring range is automatically selected when the rectangular cell (1, 2, 5 cm) is inserted. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.
photoLab® 6100 VIS Operation 4.5.3 Measuring reagent-free tests and user-defined methods User-defined methods and reagent-free methods normally do not have a barcode and therefore, no automatic method recognition. In such a case, select the method manually: Concentration Concentration 04/16/07 9:52 1 Select the method manually (see section 4.5.5). Please select method for measuring or insert a barcoded cell or insert AutoSelector.
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover. Concentration 04/16/07 9:52 0.629 1001: Nitrite Setup – Select a different citation form with [Citation form], (e.g.
photoLab® 6100 VIS Operation 4.5.
photoLab® 6100 VIS Operation 4.5.5 Selecting the method last used Selecting a method from the Method list Selecting a method manually The method last used is immediately selected. Concentration – [Last method] Concentration – [Method list] Select method (all) 04/16/07 9:52 Search: 1 2 3 4 5 6 7 160 67 39 C3/25 C4/25 A6/25 N2/25 N5/25 P6/25 P7/25 09717 14834 14552 Last used COD COD NH4-N NO3-N NO2-N PO4-P PO4-P Pb Cd Cr 10 - 150 mg/l 25 - 1500 mg/l 0.20 - 8.00 mg/l 0.5 - 25.
photoLab® 6100 VIS Operation Search function Select method (last used) 04/16/07 9:52 CO_ 14 23 14540 14541 COD COD Search for a character string: Enter the character string to be searched for in the search window with . 10 - 150 mg/l 25 - 1500 mg/l The list appearing below shows all hits containing the character string. The hit list is updated with each character that is entered. All methods Note Note the case sensitivity when searching.
photoLab® 6100 VIS Overview of the settings Operation Menu item Explanation Dilution Here you can set the dilution prior to measuring if you want to use a diluted sample. In the measured value display, the dilution is indicated in the form [1 + x] (parts sample + parts distilled water). For more detailed information on dilution, see section 4.5.7. Sample blank value Here you can measure while taking a sample blank value into account.
photoLab® 6100 VIS Operation 4.5.7 Measuring diluted samples If the concentration of a sample exceeds the measuring range of a method, you can specifically dilute the sample so that the concentration of the diluted sample is in the measuring range of the method. Thus a valid measurement is possible. After entering the factor for the dilution the meter converts the concentration to that of the undiluted sample.
photoLab® 6100 VIS Operation Concentration 04/16/07 9:52 2 Select and confirm Dilution. The input field for the dilution pops up. Sample + distilled water 1+_ 3: A6/25 NH4-N 16 mm Setup 0.20 - 8.00 mg/l Method list Citation form 1 Open the setting menu with [Setup]. Unit 3 Enter and confirm the dilution (<0...9>). The entered dilution is taken into account with the next measurement. The entered value for the dilution factor is valid for the selected method only.
photoLab® 6100 VIS Operation 4.5.8 Sample blank value By measuring and using a sample blank value, measurement errors due to coloring and turbidity of the sample matrix can be eliminated to a large extent. The sample blank value is a characteristic of the sample (coloration) to be currently determined. It is determined by measuring the blank sample. The blank sample is prepared from the sample by adding reagents to it and is especially adapted to measurement with the test to be measured.
photoLab® 6100 VIS Operation Concentration 04/16/07 9:52 The photometer is ready to measure. 1 Open the setting menu with [Setup]. To start measurement, insert cell or press 2 Select and confirm Sample blank value. 3: A6/25 NH4-N 16 mm Setup 0.20 - 8.00 mg/l Method list Citation form Sample blank value Unit 04/16/07 9:52 3 Insert the cell with a suitable blank sample. The first single measurement for the sample blank value takes place.
photoLab® 6100 VIS Operation 4.5.9 Reagent blank value The evaluation of the photometric measurement always refers to the comparison value of a test sample without the substance to be determined (reagent blank value). Thus the influence of the basic absorbance of the reagents on photometric measurement is compensated for. In practice, the reagent blank value is measured with the same amount of deionized water instead of sample.
photoLab® 6100 VIS User-defined methods Operation For user-defined methods, you can activate the reagent blank value function as follows only: Entry type Function type Reagent blank value possible? Entry of a function (with and without entering the ordinate intercept) Linear Yes Nonlinear No Entry of value pairs or measurement and storage of standard solutions (with entering or measuring and storing E0) Linear Yes Parabola (second-order function) Yes Polygon line No Entry of value pairs or
photoLab® 6100 VIS Operation Measuring the reagent blank value Concentration Inserting a cell with barcode starts a measurement. 04/16/07 9:52 Concentration If a cell without barcode is used: Select the method manually (see section 4.5.5). Please select method for measuring or insert a barcoded cell or insert AutoSelector. Setup Method list Last method New Method The photometer is ready to measure.
photoLab® 6100 VIS Operation Blank value 04/16/07 9:52 The first single measurement for the reagent blank value takes place. To start measurement, insert cell or press 3: A6/25 NH4-N 16 mm 3 Insert the cell with the blank sample. 0.20 - 8.00 mg/l The following data is displayed as the result: – The measured absorbance from the (last) single measurement. – The median from all single measurements carried out up to now. 04/16/07 9:52 Blank value Last measured absorbance 0.
photoLab® 6100 VIS Operation 4.5.10 Automatic Turbidity correction The Turbidity correction function activates the automatic recognition and compensation of the light absorption caused by turbid substances. After activating the function remains permanently switched on. Measured values that were measured with Turbidity correction are labeled with [TURB] (turbidity correction) on the display and in the documentation (printout and memory).
photoLab® 6100 VIS The dependency between the nominal value and absorbance is often linear in a wide range as shown in the following example: Absorbance Line types Operation 0 0 Nominal value (e. g. concentration) Bild 4-2 Example of a linear calibration function after a 10-point calibration In the case of a linear dependency, the calibration function is determined by means of linear regression. The slope and axis intercept (E0) are the characteristics of the calibration line.
photoLab® 6100 VIS Operation Determining the calibration function You have the following options to create a method: z Measure and store: Carry out a series of measurements with the following sample solutions while at the same the photometer takes over the values: – Blank sample to determine the reagent blank value (with deionized water instead of sample, see section 4.5.9) – at least one, up to ten standard solutions in different concentrations.
photoLab® 6100 VIS Operation If the exact value for a0 is not known at the time the formula is entered, it is sufficient to enter the coefficient a1. In this case, the User-defined blank value function (in the Concentration / Setup menu) has to be activated to measure with this method. Prior to measuring with this method, a blank value measurement has to be carried out in this case. This procedure determines the value for a0, which then replaces the value from the programming of the method.
photoLab® 6100 VIS Operation Further method data Input field Possible entries Number* 1001 ... 1100 Designation Any name (max. 18 characters) Version Any version designation (max. 18 characters) Wavelength* Freely selectable (in nm) Cell* 16 (round), 10, 20 or 50 mm Citation form e.g. PO4-P (max. 18 characters) Unit** e.g. mg/l (max. 18 characters) Resolution* 0.001, 0.01, 0.
photoLab® 6100 VIS How to program user-defined methods Operation Concentration – [Setup] – New method Edit method 04/16/07 9:52 Number Designation Version Wavelength Cell Citation form Unit Resolution Calibration curve Method list 1001 Nitrite 01 525 10 mm NO2-N mg/l 0.001 Measure standard solutions Delete Next 1 Enter the general method data here. The next available method number is already entered as the number.
photoLab® 6100 VIS Operation Variant 1: Measure standard solutions Edit method 04/16/07 9:52 Standard ID Standard manufacturer Back Enter and confirm details of the standard solutions (optional). 3 Using [Next], accept all entries on the page and switch to the next page. The table for the measurement of standard solutions pops up.
photoLab® 6100 VIS Operation Absorbance E0 04/16/07 9:52 The measurement display appears. 7 Insert the cell with the respective standard. To start measurement, insert cell or press The absorbance is measured. The result of the first single measurement is displayed. 525 nm 16 mm Absorbance E0 04/16/07 9:52 Last measured absorbance 0.009 Median 0.009 (1 Measurement(s)) 525 nm Next meas.
photoLab® 6100 VIS Operation Edit method 04/16/07 9:52 f(x)=0.73x R2 = 1.000 Meas. range: 0.050 - 1.000 mg/l The related formula f(x) and correlation coefficient R2 are displayed above the diagram. Absorbance 12 If required, select a different line type for the line adjustment with [Curve type]. - Polygon line - Straight line Concentration [mg/l NO2-N] Back Curve type Meas. range Next - Parabola 13 If required, enter different measured value limits with [Meas. range].
photoLab® 6100 VIS Variant 3: Enter formula Operation Edit method 16.04.07 9:52 c = a0 + a1·A + a2·A2 + a3·A3 + a4·A4 + a5·A5 a0 a1 a2 a3 a4 a5 Lower limit of measuring range Upper limit of measuring range Method list Delete 0.605 2 1,000 mg/l 3.000 mg/l 1 Select and confirm Enter formula. Input fields for the coefficients (a0 ... a5) of the formula are displayed. 2 Enter and confirm the factors. If no value is entered for a coefficient the photometer automatically uses the value 0.
photoLab® 6100 VIS Operation 4.5.12 The IQ LabLink procedure The IQ-LabLink procedure enables the secure data exchange between the sensors of the IQ SENSOR NET online measuring system and a photometer (such as the spectroFlex series) with the aid of a commercial USB memory device. During the matrix adjustment of the sensor, the measurement data of the IQ SENSOR NET sensor are adjusted to the photometrically determined reference data.
photoLab® 6100 VIS Operation 4.6 Measuring the Absorbance / % Transmission 4.6.1 General information The absorbance or transmission respectively is measured without the use of any methods or profiles. All settings are configured during measurement. Measuring against the Reference absorbance The absorbance or transmission can alternatively be measured against the absorbance of the zero adjustment or against a Reference absorbance determined by yourself (see section 4.6.
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover. Absorbance The photometer starts measuring automatically. 04/16/07 9:52 0.
photoLab® 6100 VIS Operation 4.6.3 Measuring against the Reference absorbance Each time the photometer is switched on, the absorbance or transmission is measured against the absorbance of the zero adjustment as a basis. You can, however, also determine a Reference absorbance and use it as the basis. The Reference absorbance refers to the adjusted wavelength.
photoLab® 6100 VIS Operation Reference absorbance 04/16/07 9:52 2 Insert the cell with the reference sample. The first single measurement for the Reference absorbance is carried out. To start measurement, insert cell or press The following data is displayed as the result: 489 nm 10 mm – The measured absorbance from the (last) single measurement. – The median from all single measurements carried out up to now.
photoLab® 6100 VIS Operation 4.7 Multi wavelengths methods 4.7.1 Basic information on Multi wavelengths measurements The multi wavelength function of the photoLab® 6100 VIS enables free calculation of any parameters from the absorbance values of several wavelengths. When doing so, two to ten wavelengths can be used. Calculation takes place based on the following equation: R= a 0 + a1 ⋅ A1 + a 2 ⋅ A2 + ... + a10 ⋅ A10 b 0 + b1 ⋅ A1 + b 2 ⋅ A2 + ...
photoLab® 6100 VIS Operation 4.7.2 Programming / modifying Multi wavelengths methods Note For multi wavelength methods, the method numbers 2001 to 2050 can be used. In the delivery condition, one method is stored for demonstration purposes. Multi wavelengths – [Setup] – Edit method Edit method (1 of 6) Number Name Version Citation form Unit Resolution Cell Method list 04/16/07 9:52 1 2001 PROT 1.0 Protein mg/ml 0.1 10 mm Delete Page 1 of 6: Enter the general method data here.
photoLab® 6100 VIS Operation Edit method (2 of 6) 04/16/07 9:52 Wavelength 1 Wavelength 2 280 nm 260 nm 3 Page 2 of 6: Add another wavelength with [Add]. Delete the last wavelength with [Delete]. 4 Using [Next], accept all entries on the page and switch to the next page. Back Add Delete Edit method (3 of 6) D Next 04/16/07 9:52 a0 + a1 * A1 + a2 * A2 + ... + a10 * A10 R= b0 + b1 * A1 + b2 * A2 + ... + b10 * A10 a0 a1 a2 a3 a4 a5 0.000 1.550 -0.757 0.000 0.000 a6 a7 a8 a9 a10 0.000 0.000 0.
photoLab® 6100 VIS Operation Edit method (6 of 6) 04/16/07 9:52 1.550 * A(280 nm) - 0.757 * A(260 nm) R= 1.000 10 Page 6 of 6: The basic equation is displayed (for more information on the basic equation see section 4.7.1). 11 Complete the editing with [Complete].
photoLab® 6100 VIS Operation 4.7.3 Selecting a Multi wavelengths method To select a method for Multi wavelengths measurements, proceed as follows: Multi wavelengths – [Method list] Select method (all) 2001 Protein 04/16/07 9:52 Protein mmol/l 2002 DNA purity The list of methods is displayed. The methods are ordered according to the method number. Select the method: 1 Select the required method with <▲><▼>. The active selection is displayed in reverse video.
photoLab® 6100 VIS Operation 4.7.4 Carrying out Multi wavelengths measurements Multi wavelengths Multi wavelengths 04/16/07 9:52 1 Select the required method with [Method list] (see section 4.7.3). Please select method for measuring! Setup Method list Transmission Multi wavelengths The photometer is ready to measure after the method has been selected.
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover. Multi wavelengths 04/16/07 9:52 If necessary, carry out a zero measurement.
photoLab® 6100 VIS Operation 4.8 Spectrum 4.8.1 General information With the Spectrum function, the absorbance or Transmission in dependency of the wavelength is measured and recorded. The wavelength range can be freely selected within the measuring range of the photometer. The increment is 1 nm. A spectrum is recorded without using any methods or profiles. All settings are configured during measurement. Baseline A baseline has to be recorded before a spectrum is recorded.
photoLab® 6100 VIS Operation 4.8.2 Recording the Spectrum Spectrum Absorbance Spectrum 04/16/07 9:52 A message containing operating instructions is displayed. Spectrum You have to record a baseline first (). Adjustment of wavelength range under . Wavelength [nm] Setup Open 1 Open the setting menu with [Setup].
photoLab® 6100 VIS Operation Spectrum Recording the baseline: 04/16/07 9:52 4 Press the key. Absorbance The photometer records the baseline. 5 Wait until the baseline is completely recorded. Wavelength [nm] Spectrum The photometer is ready to measure after the baseline has been recorded.
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. 9 Close the cell shaft cover. 10 Start the measurement with . Spectrum 04/16/07 9:52 11 Wait until the spectrum is completely recorded. Absorbance At the end of the recording the following message appears: Recording of spectrum is completed.
photoLab® 6100 VIS Operation 4.8.3 Loading/editing a spectrum A spectrum can be edited immediately after measurement. Stored spectra can be loaded and edited as well. The following tools are available for editing: z Cursor function for incremental moving along the curve with indication of the x and y values z Zoom function to scale up a section z Mathematical functions for various evaluating and calculating operations. The functions are described from page 86.
photoLab® 6100 VIS Spectrum 04/16/07 9:52 1 Press [Zoom]. The zoom window appears. The bottom left corner of the zoom window is marked by a small black square. Absorbance Zoom Operation – You can return to the original view of the spectrum with [Original] at any time. Wavelength [nm] Setup xy min Spectrum 04/16/07 9:52 2 Adjusting the zoom window: Absorbance – Define the bottom left corner of the zoom window with and <▲><▼>.
photoLab® 6100 VIS Operation Edit Open the selection of mathematical functions with [Edit]: z Extreme values (zoomed area) Highlights the extreme values (minimum and maximum values) of the displayed spectrum. z Mark points Opens an edit mode where you can highlight individual points of the spectrum. With the [Mark] function key you can highlight individual points. The wavelength and measured valued are displayed at the highlighted point. With the [Delete] function key you can remove individual points.
photoLab® 6100 VIS Operation 4.8.4 Saving / exporting a spectrum The saving of a spectrum saves both the edited and the original spectrum. Consequently, the original spectrum can be restored from each stored spectrum. 04/16/07 9:52 Spectrum Absorbance Saving 1 Record a spectrum (see section 4.8.2) or Load a stored spectrum (see section 4.8.3). 2 If necessary, connect a USB memory device to the USB-A interface. Wavelength [nm] Setup Edit Zoom Open 3 Open the save dialog with .
photoLab® 6100 VIS Operation 4.9 Kinetics The Kinetics function enables the temporal tracing of the absorbance or transmission of a sample at a certain wavelength. The photometer automatically calculates the slope between two adjacent measuring points from the available measurement data. The catalytic activity can also be determined and displayed if required.
photoLab® 6100 VIS Operation Input field Possible entries Scaling: Auto** During measurement, the instrument adjusts the axis scaling (minimum and maximum value of the axis) to the measured values. The entire curve is always visible. Scaling:Manual The axis scaling (minimum and maximum value of the axis) is set manually. Y-axis min Y-axis max Measurements/interval 1/interval or Max/interval Here you define how many measurements are carried out per interval.
photoLab® 6100 VIS Operation Creating/editing a profile Kinetics – [Setup] – Edit profile Edit profile (1 of 2) Number Name Mode Wavelength Duration Interval Delay Scaling Profile 04/16/07 9:52 1 4001 NADH Absorbance 340 nm 02:00:00 00:00:30 00:01:00 Auto Delete Enter the data for the profile here. The next available profile number is already entered as the number.
photoLab® 6100 VIS Operation 4.9.2 Loading a profile for kinetic recording To load a profile for kinetic recording, proceed as follows: Kinetics – [Profile] Select profile (all) 04/16/07 9:52 4001 NADH Absorbance 4002 ADH Absorbance The list of profiles is displayed. The profiles are ordered according to the profile number. Selecting a profile: 1 Select the required profile with <▲><▼>. The active selection is displayed in reverse video. 2 Accept the selection with .
photoLab® 6100 VIS Operation 4.9.3 Recording the Kinetics Note During the recording, the photometer cannot carry out any regular self-test or self-calibration (AutoCheck), because the recording would have to be interrupted for this. A warm-up time of at least two hours is required for the photometer to measure reliably during the recording. Kinetics Kinetics Note the warm-up time of at least 2 hours for kinetic recordings.
photoLab® 6100 VIS Operation Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back. The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover. The photometer starts recording automatically.
photoLab® 6100 VIS Operation Cursor information 4 Kinetics 04/16/07 9:52 Number of measurements: 4 Interval:00:00:06 Absorbance 4002 Duration: 00:00:24 Time [s] Setup Profile Edit Open After the specified Duration has expired, the cursor appears. You have the following options: - You can move the cursor along the curve and have the measurement data for each point displayed (see section 4.9.6) - With , you can output the kinetic curve to a connected printer as a graphic.
photoLab® 6100 VIS Operation 4.9.4 Saving Saving / exporting a kinetic record Kinetics 04/16/07 9:52 Number of measurements: 4 Interval:00:00:06 Absorbance 4002 Duration: 00:00:24 1 Carry out the kinetic recording (see section 4.9.3) or Load a stored kinetic record (see section 4.9.4). 2 If necessary, connect a USB memory device to the USB-A interface. Time [s] Setup Setup Profile Profile Edit Slope Open Open 3 Open the save dialog with .
photoLab® 6100 VIS Operation 4.9.5 Loading a kinetic record You can load and view stored kinetic records. Loading a stored Kinetics record Kinetics – [Open] The list with the stored kinetic records is displayed (Internal DataB folder). 04/16/07 26.02.07 Enzyme kinetics.csv 24.02.07 ADH.csv 24.02.07 kinetics_4002_070224_1410.
photoLab® 6100 VIS Operation 4.9.
photoLab® 6100 VIS Operation Note The Slope function is only available if the kinetic recording was done in the Absorbance mode.
photoLab® 6100 VIS Operation 4.10 Timer You can use the timers to remind you by an acoustic signal of a time interval that has expired. The photometer has two types of timers: z The User defined timer is a timer that can be freely assigned. The interval and name can be freely set. Only one freely assignable timer is available. It cannot be erased (see section 4.10.1). z Analysis timer are timers permanently stored in the photometer.
photoLab® 6100 VIS Operation 4.10.1 User defined timer If you want to manually enter time intervals, use the User defined timer function. Timer 04/16/07 9:52 Designation User defined timer Time 00:15:00 Status Inactiv A6/25 - 1 00:15:00 Inactiv Start Stop Edit The Timer menu is open. 1 Highlight the User defined timer. 2 If necessary, change the name and time of the timer with [Edit]. 3 Start the highlighted timer with [Start]. The status of the timer is Active.
photoLab® 6100 VIS Operation 1 Select the required method in the Concentration mode. Manual selection of the method (see section 4.5.5). 2 Open the Timer menu. Timer 04/16/07 9:52 Designation Time Status User defined timer A6/25 - 1 00:15:00 00:15:00 Inactiv Inactiv Start Stop Remove Add The Timer menu is open. 3 If necessary, add a new timer to the list with [Add].
photoLab® 6100 VIS Operation 4.11 Memory 4.11.1 Overview Concentration AQA records Spectrum Absorbance / % Transmission Kinetics Multi wavelengths or AutoStore 3 Measured value memory (Filter optional) *.csv PC + SpectralTransfer Open in source application Internal folder DataB *.
photoLab® 6100 VIS Operation Measurement data Save, back up, export Concentration, Measurement datasets of these measuring modes are first stored in the measured value memory of the photometer (1000 memory locations) with or AutoStore. Absorbance / % Transmission Multi wavelengths The measured value memory is available from the Measurement data memory menu. Here you can view, filter and export into a PCreadable file (*.csv) the stored measurement datasets ().
photoLab® 6100 VIS Operation 4.11.2 Instructions on using USB memory devices The safety of data stored on USB memory devices depends on the quality of the memory device and the data transmission. Data is stored partly or not at all if for example: z The power supply of the external memory device is interrupted during the write process, or z The external memory device is prematurely disconnected from the photometer during the data backup.
photoLab® 6100 VIS Operation 4.11.3 Measurement datasets Elements of a measurement dataset A complete measurement dataset consists of: z Consecutive number (is automatically assigned by the photometer) z Date/time z Identification (e.g. ID or "AutoStore") z User name z Measured parameter, e.g.
photoLab® 6100 VIS Operation 4.11.5 Saving measurement datasets automatically For the measuring modes, Concentration, Absorbance / % Transmission, and Multi wavelengths you can record every measured value automatically (AutoStore). All automatically stored measurement datasets are given the ID "AutoStore". The "AutoStore" ID is overwritten if the same measured value is manually stored afterwards (). This ensures that every measurement dataset is stored in the data memory only once.
photoLab® 6100 VIS Operation 4.11.
photoLab® 6100 VIS Operation Each of these options indicates the contents of the measurement data memory as a list as follows. Measurement data memory 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 14:00 14:05 14:10 14:15 14:20 14:25 14:30 14:35 14:40 3.50 mg/l Ni 3.64 mg/l Ni 3.69 mg/l Ni 3.72 mg/l Ni 3.72 mg/l Ni 3.75 mg/l Ni 3.73 mg/l Ni 3.80 mg/l Ni 3.
photoLab® 6100 VIS Operation 4.11.7 Filtering measurement datasets The functions to display, delete and download stored measurement datasets refer to all stored measurement datasets that correspond to the specified filter criteria. Filter criteria The following filter criteria can be set: z Mode (measured parameter) z User z ID (identification) z Date (date from ... to ...
photoLab® 6100 VIS Operation Note Alternatively, you can hide measurement datasets that meet the specified filter criteria with the Selected values: invert selection function (see section 4.11.8). 4.11.8 Inverting filters With the Selected values: invert selection function you can hide all measurement datasets that correspond to the specified criteria of the filter (see section 4.11.7). Note You can use this function to select and delete measurement datasets no longer used.
photoLab® 6100 VIS Operation 4.11.9 Erasing stored measurement datasets If you no longer need any stored measurement datasets, you can erase them individually or altogether. Concentration, Absorbance / % Transmission, or Multi wavelengths – [Setup] – Measurement data memory Measurement data memory 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 27.03.07 14:00 14:05 14:10 14:15 14:20 14:25 14:30 14:35 14:40 3.50 mg/l Ni 3.64 mg/l Ni 3.69 mg/l Ni 3.72 mg/l Ni 3.
photoLab® 6100 VIS Operation 4.12 Copying files If you want to back up measurement data files outside the photometer, there are several ways to copy them to external media. Note Please follow the instructions on using USB memory devices (see section 4.11.2). 4.12.1 Copying individual files to a USB memory device After the following measurements, the Save dialog opens and prompts you to save the data in a *.csv file: z Kinetics z Spectrum z AQA3/MatrixCheck If the data are not saved in *.
photoLab® 6100 VIS Example: Saving data from the measured value memory Operation Concentration, Absorbance / % Transmission, or Multi wavelengths – [Setup] – Measurement data memory 1 If necessary, set the filter criteria with [Setup]. 2 Open the save dialog with . The photometer automatically proposes the location Internal DataB folder and a file name. Save (Internal DataB folder) MData_1.csv 04/16/07 9:52 3 If necessary, change the location with [Location] (USB memory).
photoLab® 6100 VIS Operation 4.12.2 Copying all measurement data files to a USB memory device Even if no PC is directly connected to the photometer, you can very simply transfer all measurement data files from the photometer (Internal DataB folder) to a connected USB memory device. [Setup] – Save all data on USB memory A message appears while the data are being saved. When the data saving procedure is finished, a message appears. 1 Confirm the message with .
photoLab® 6100 VIS Operation 4.12.3 Copying files to a PC After saving measurement data in csv format you can copy them to a PC. Measurement data in csv format can be directly imported to and processed in spreadsheets such as Microsoft® Excel®. Note Depending on the country variant, some spreadsheet programs require a certain decimal separator for the correct import of numerical values (comma or point).
photoLab® 6100 VIS Operation Usable printers 4.13 Transmitting data 4.13.1 Printer and terminal programs Data can be printed with the following printers: z Matrix printer connected to the RS232 interface z Standard printer (ink or laser) connected to the USB-A interface Note Suitable are all printers that can interpret the PCL-3 printer control language. The printer symbol indicates that the display contents can be printed. To print, press .
photoLab® 6100 VIS Operation 4.13.3 Printing measurement datasets This section describes how to print measurement datasets of the measuring modes, Concentration, Absorbance / % Transmission, and Multi wavelengths. By means of sample printouts, the printed information is described below: Concentration and Multi wavelengths mode 21 05.06.07 14:05:41 C4/25 844 mg/l COD Inlet Administrator 0.005 02.06.07 11:02:13 2 AQA1: 9 AQA2: 14 Structure of the lines from left to right: 1st line: [Consecutive no.
photoLab® 6100 VIS Operation 4.13.4 Printing Kinetics records Sample printout SpectroFlex 6100 09130512 1.30-WTW-1.60 Administrator 05.06.07 12:14:55 320 nm Time [s] 6 17 25 35 45 .. .. (etc.) Structure of the lines from left to right Absorbance 0,092 0,077 0,073 0,077 0,071 ..... .....
photoLab® 6100 VIS Operation 4.13.5 Printing spectra Sample printout SpectroFlex 6100 09130512 1.30-WTW-1.60 Administrator 07.06.07 09:47:00 Wavelength [nm] 320 321 322 323 324 ... ... (etc.) Structure of the lines from left to right Absorbance 0,238 0,240 0,241 0,240 0,239 ..... .....
photoLab® 6100 VIS Operation 4.14 Analytical quality assurance (AQA) 4.14.1 General information The target of the analytical quality assurance (AQA) is to secure correct and precise measurement results. Note Settings for AQA checks are only available for users of the user group, administrator. Every registered user can carry out the AQA check (see also section 4.15.1).
photoLab® 6100 VIS Operation 4.14.2 Photometer monitoring (AQA1) At least one set of test standards such as Spectroquant® PhotoCheck or CertiPUR® is required for the photometer monitoring. The administrator specifies which test standard has to be used as the minimum requirement for the AQA1 monitoring. The extent of the monitoring can be enlarged with further test standards. Note Settings for AQA checks are only available for users of the user group, administrator.
photoLab® 6100 VIS Operation Activating AQA1 The AQA1 monitoring is activated in the Mode menu: [AQA] – AQA1 setup – Mode Mode Interval Lock instrument Mode Configuration of tests ... Select and confirm Weeks. 04/16/07 9:52 AQA1 setup AQA1 is active. The Interval setting indicates Weeks as the interval unit. Weeks AQA1 inactive Weeks Defining the AQA1 Interval The AQA1 Interval defines the interval between two AQA1 checks.
photoLab® 6100 VIS Configuring the lock of the photometer Operation Here you configure whether or not the photometer will be locked against all measurements if there is no valid AQA1 check or the interval for the AQA1 check has expired. [AQA] – AQA1 setup – Lock instrument AQA1 setup 04/16/07 9:52 Mode Weeks Interval Lock instrument Lock instrument Shouldofthe instrument be locked for further Configuration tests ...
photoLab® 6100 VIS Operation PhotoCheck 04/16/07 9:52 Lot number: Use by HC616115 16.04.2008 Tolerance ± 0.020 ± 0.030 ± 0.040 ± 0.050 ± 0.020 ± 0.030 ± 0.040 ± 0.050 445/1 445/2 445/3 445/4 525/1 525/2 525/3 525/4 Target value 0.196 0.500 0.998 1.508 0.197 0.495 0.992 1.496 Apply Carrying out the AQA1 check (example: PhotoCheck) Example, PhotoCheck: 5 Using <▲><▼> and , select the Lot number, Target value or Tolerance entries and open them for editing with .
photoLab® 6100 VIS Operation PhotoCheck 445/1 04/16/07 9:52 2 Insert the cell. The cuvette is automatically recognized and the measurement started. Please insert PhotoCheck 445/1 After measuring, the measurement result, Target value, Tolerance and an evaluation (OK or failed) are displayed. The photometer offers to repeat the measurement if the check failed. If the check was successful, the measurement of the next PhotoCheck test standard, e.g. 445/2, appears on the display.
photoLab® 6100 VIS Operation 4.14.3 Total system monitoring (AQA2) For the total system monitoring, standard solutions with a defined analyte content are required (preferably certified Spectroquant® CombiCheck standards). Note Settings for AQA checks are only available for users of the user group, administrator. The AQA check can be carried out by any registered user. Spectroquant® CombiCheck Spectroquant® CombiCheck standards are multiparameter standards ready to use, i. e.
photoLab® 6100 VIS General AQA2 settings Operation [AQA] – AQA2 setup 04/16/07 9:52 AQA2 setup Mode Weeks Lock methods Method... Yes 1 Select and confirm Mode. The Mode selection field pops up. 2 Select and confirm Weeks or Measurements. AQA2 is active. For all methods, the AQA2 intervals are entered either in weeks or number of measurements. Method list 3 Accept the general settings with [Apply].
photoLab® 6100 VIS Operation Activating AQA2 monitoring for a method [AQA] – AQA2 setup – Method... AQA2 setup Method 04/16/07 9:52 1 Select a method (see section 4.5.3). 3: A6/25 AQA2 AQA2 active Interval Target value 12 Weeks 4.00 mg/l NH4-N 2 Select and confirm AQA2. Tolerance Standard ID 0.50 mg/l NH4-N 3 Select and confirm AQA2 active. AQA2 is active for this method.
photoLab® 6100 VIS Carrying out the AQA2 check for a method Operation [AQA] – AQA2 check AQA2 check Target value 04/16/07 9:52 2.00 2 Insert the cell or start measurement with . To start measurement, insert cell or press 3: A6/25 16 mm 1 Carry out the check like a normal measurement (see section 4.5.1 to 4.5.3). NH4-N 0.20 - 8.00 mg/l After the measurement is completed, the result and its evaluation are displayed.
photoLab® 6100 VIS Operation 4.14.4 AQA3/MatrixCheck The MatrixCheck is used to check if the photometric determination is disturbed by other substances present in the sample (sample matrix). The MatrixCheck can be carried out by spiking or diluting: The photometer enables a simplified MatrixCheck with the aid of the Spectroquant® CombiCheck R-2 addition solution. The MatrixCheck can be carried out immediately. The volumes required for the sample and standards are displayed on the screen.
photoLab® 6100 VIS Practical instructions Operation z After evaluating the measured value of the sample the photometer suggests for the MatrixCheck to spike or dilute the sample and standard with suitable volumes. You can change the suggested values of the volumes for the sample and standard. The photometer checks your entries and informs you of errors (e.g. if a nominal value is outside the measuring range of the test). For each spiking or dilution, the relevant nominal concentration value is displayed.
photoLab® 6100 VIS Operation AQA3/MatrixCheck setup 04/16/07 9:52 Maximum difference 1 10% Enter and confirm a numerical value. The setting is active. Maximum difference 10.0 % Carrying out the MatrixCheck 04/16/07 9:52 Concentration 45 mg/l 18: C3/25 Exit the menu with . 1 Measure the original sample without spiking or diluting it (see section 4.5.1 to 4.5.3). 2 The measured value is displayed. 3 Open the setting menu with [Setup]. 4 Select and confirm AQA.
photoLab® 6100 VIS Operation MatrixCheck (Spike) 04/16/07 9:52 Method Sample concentration 1: C3/25 45 mg/lCOD Standard ID Standard concentration COD 1500 400 mg/lCOD Sample [ml] Standard [ml] Target value [mg/l] 10 10 10 0.5 1 1.5 62 77 91 Dilute 7 In theStandard ID entry field, select the simplified MatrixCheck with the CombiCheck standard solution or enter a designation for another standard solution used. If the CombiCheck is selected, no more entries are required (continue with step 10).
photoLab® 6100 VIS Operation MatrixCheck 04/16/07 9:52 Method Sample concentration Sample Standard 1: C3/25 45 mg/lCOD 10 ml 0.5 ml The measurement display appears. 12 Insert the cell with the respective sample. The sample is measured. To start measurement, insert cell or press 16 mm Back MatrixCheck 04/16/07 9:52 Method Sample concentration 1: C3/25 45 mg/lCOD Sample [ml] Standard [ml] Target value [mg/l] nominal [mg/l] 10 10 10 0.5 1 1.
photoLab® 6100 VIS Operation Concentration 04/16/07 9:52 [MC] 45 mg/l 18: C3/25 COD 16 mm Setup Test record 10 - 150 mg/l Method list Citation form The display returns to the measured value display of the original sample without spiking / dilution. The [MC] status indicator is displayed. A MatrixCheck was carried out for this measured value. Unit The result of the MatrixCheck is displayed in a test record. You can print this record and save it as a file.
photoLab® 6100 VIS Operation 4.15 User management The functions of the user management are only available for users of the user group, Administrator. An administrator can z activate or deactivate the user management for the meter z create, change or delete individual user accounts. 4.15.1 User levels and user rights The photoLab® 6100 VIS allows the management of up to 100 users. Every user is member of a user group with defined user rights.
photoLab® 6100 VIS Operation Note You can also switch off the user management and reactivate it as necessary. To do so, you need administrator rights. If the user management is switched off, the user name and password do not have to be entered. Each user has full rights. 4.15.2 Activating or deactivating the User management function Each user can activate the user management function. If the function is deactivated, each user has administrator rights.
photoLab® 6100 VIS Operation Deactivating the user management function [General setup] – User management – [Setup] – Deactivate user management The user management function is inactive. Each user has administrator rights. Note If the user management is deactivated by a user of the Administrator user group, all user accounts that were set up are lost. The password for the administrator is reset to "admin". 4.15.
photoLab® 6100 VIS Operation User management 04/16/07 9:52 Name User group Administrator Administrator Admin2 Administrator Enter user name The input field for the new user name pops up. 1 Enter the user name () and confirm. A_ The selection field for the user group (Administrator / User) pops up. 2 Select and confirm the user group. Setup Add Delete Change The input field for the password pops up. 3 Enter the password () and confirm.
photoLab® 6100 VIS Operation Deleting a user account [General setup] – User management 1 Select a user account. 2 Delete the user account with [Delete]. A security prompt appears: Confirm deletion ? 3 Confirm the security prompt. The user account is deleted.
photoLab® 6100 VIS Operation 4.15.4 Login with active user management To be able to always assign measurement data to a user, the administrator can activate the user management function. After doing so, the photometer can only be operated after login with a user name. Depending on the authorization class (administrator, user, guest), important settings are released for changes or locked. Note The user management function is not active in the delivery condition of the photoLab® 6100 VIS.
photoLab® 6100 VIS Operation The photometer is switched on. The Login dialog is displayed. Login 04/16/07 9:52 1 The input field for the password pops up. Enter user name Administrator Login admin 142 If the user name is not known (or incorrectly spelled) it is possible to log in without a password as a guest with restricted rights (see section 4.15.1). 04/16/07 9:52 Enter password Enter the user name () and confirm. 2 Enter the password () and confirm.
photoLab® 6100 VIS Operation 4.15.5 Changing the password The administrator sets up user accounts and assigns a password to each user account. As soon as any user has successfully logged in with the password, they can change the password for their user accounts themselves. [General setup] – User management – Change password User management Old password 04/16/07 9:52 1 Enter and confirm the old password. 2 Enter and confirm the new password. The password is changed.
photoLab® 6100 VIS Operation 4.16 Reset You can reset (initialize) the measurement settings or all settings. Note The Reset function is available for users of the user group, Administrator only. You have the following options of resetting the photometer settings: z Reset configuration All settings except for the measurement data memory, userdefined methods and measured blank values are deleted.
photoLab® 6100 VIS Operation 4.17 Photometer information ([Info]) The following photometer information is displayed: z Photometer designation z Version number of the meter software/method data z Hardware version z Series number of the meter z Registered user z Hardware status (for service purposes) z Memory status [Info] Info 04/16/07 9:52 Model designation: Serial number: photoLab® 6100 VIS 07440001 Software/methods version: 1.30-WTW-1.
photoLab® 6100 VIS Operation 4.19 Software and methods update The software and method update is used to continuously update your photometer. Note Only users of the user group, Administrator are allowed to carry out a software and method update. The update comprises z the newest firmware (meter software) z new or changed method data Note User-defined data (such as settings, user-defined methods or measured data) are not changed by a software and methods update.
photoLab® 6100 VIS Operation Note Make sure the folder structure of the files is retained while the files are unpacked. If you use a program such as WinZip to unpack the files, the option, "Nutze Ordnernamen" or "Use Folder Names" must be set. Details are given in the documentation of the unpack program. The "Update" folder must be on the top level of the USB memory device. The Update folder contains several subfolders. The following steps are carried out at the photometer.
photoLab® 6100 VIS Operation 4.19.2 Update by means of a PC Requirements The following is required for the update: z A free USB connection on the PC z A USB cable (type A - type B) z The SpectralTransfer program. It is on the CD-ROM provided with the photometer. z The ActiveSync® program. It is available free of charge in the download area of Microsoft on the Internet. z The current photometer update file. It is available on the Internet under http://www.WTW.com.
photoLab® 6100 VIS Operation 4.19.3 Software update "Chinese character set" If you want to display on your photometer the languages, "Simplified Chinese/ 中文 " or "Traditional Chinese/ 繁體中文 ", a Chinese character set is required to display the Chinese characters. This character set is not preinstalled. You can install on your photometer this character set with the software update, "Chinese character set". It permanently requires 2 MB free storage on your photometer.
Operation 150 photoLab® 6100 VIS ba75847e01 08/2009
photoLab® 6100 VIS Service life of the tungsten halogen lamp Maintenance and cleaning 5 Maintenance and cleaning 5.1 Replacing the lamp The tungsten halogen lamp is a wear part with a certain average service life (see chapter 7 TECHNICAL DATA). It has to be replaced if defective. The photometer has a service hour counter for the lamp module (see section 4.18). Note The replacement lamp is readily assembled as a lamp module and optically adjusted in the factory. Therefore, treat it with utmost care.
photoLab® 6100 VIS Maintenance and cleaning 3 Disconnect the electrical plug connection (3). Disconnecting it might take a lot of effort. 4 Unscrew the two knurled-head screws (4) and remove the defective lamp module (5). 5 Fix the new lamp module with the knurled-head screws. When doing so, the metal-plated side of the PCB must point outward, i. e. toward the knurled-head screws. 6 Reconnect the electrical plug connection. 7 Fix the lamp cover.
photoLab® 6100 VIS Maintenance and cleaning 5.2 Exchanging the buffer batteries Caution There is a risk of explosion if unsuitable batteries are used. Only use leakproof alkaline manganese batteries. Note If you leave the photometer switched on during the exchange or insert the new batteries within a minute after taking out the old ones, the date and time are retained in the photometer. 1 1 Turn the photometer upside down and place it on a soft surface. 2 Open the lid of the battery compartment (1).
photoLab® 6100 VIS Maintenance and cleaning 5.3 Cleaning Caution The housing components are made out of synthetic materials (ABS, PMMA and PC). Thus, avoid contact with acetone, ethyl alcohol and similar detergents that contain solvents. Any splashes must be wiped off immediately. Clean your photometer as follows: z If the housing surface is dirty, wipe it with a soft cloth and mild soapy water. z Remove any chemicals splashes as soon as possible.
photoLab® 6100 VIS What to do if ... 6 What to do if ... 6.1 Actions in the case of a broken cell Warning Cells can contain dangerous substances. If the contents are released, follow the safety instructions of the package insert. If necessary, take corresponding protective measures (protective goggles, protective gloves etc.). Caution Do not turn the photometer upside down to remove the liquid! When doing so, the liquid could come into contact with electronic components and damage the photometer.
photoLab® 6100 VIS What to do if ... 6.2 Instrument does not react to keystroke Error causes and remedies Cause Remedy – Software error – Operating condition undefined or EMC load unallowed – Processor reset: Press the and key simultaneously.
photoLab® 6100 VIS Obviously incorrect measured values What to do if ... Cause Remedy – Cell contaminated – Clean the cell – Dilution set incorrectly – Set the dilution – Selected method not suitable – Select different method – Zero measurement incorrect – Perform zero measurement – Blank value incorrect – Remeasure the blank value – Cells that are not recognized (e.g.
What to do if ...
photoLab® 6100 VIS Measuring principle Light source Monochromator Photometric measurement Technical data 7 Technical data 7.1 Measurement characteristics Single-beam spectrophotometer Lamp type Tungsten halogen lamp Durability approx. 1000 h Type Grating monochromator with step motor Wavelength range 320 - 1100 nm Max. scan speed approx. 3300 nm/min Wavelengths calibration Automatic Accuracy ± 1 nm Reproducibility ± 0.5 nm (checkable, e.g.
photoLab® 6100 VIS Technical data Cells to be used Round cells – Outer diameter: 16 mm – Inner diameter: 13.6 mm – Flat cell bottom Rectangular cells * – Path length: 10 mm, 20 mm and 50 mm – Maximum width: 12.
photoLab® 6100 VIS Measuring modes Technical data z Concentration – Measurement with permanently programmed methods, adapted to the WTW test set program – Automatic method selection if test sets with barcodes are used – Program support for the creation of additional user-defined methods (max.
photoLab® 6100 VIS Technical data 7.2 Memory for measured values Measured value documentation and quality assurance Memory capacity – 1000 single measured values from the measuring modes, concentration, absorbance / % transmission and multi wavelengths – 4 MByte internal memory, sufficient for approx.
photoLab® 6100 VIS Technical data 7.3 General meter data Dimensions 404 x 197 x 314 mm (width x height x depth) Weight approx. 4.
photoLab® 6100 VIS Technical data Communication interfaces RS232 1 x 9-pin D-sub USB – 1 x USB-A (for printer, USB memory devices, keyboard or bar code reader) – 1 x USB-B (for PC) Other features z Drain for spilled cell contents z Photometer software update and method data update possible via Internet Available languages z Deutsch z English z Français z Español z Italiano z Bulgarian/Български z Česko z Simplified Chinese/ 中文 z Traditional Chinese/ 繁體中文 z Greek/Ελληνικά z Indonesian/Indonesia z J
photoLab® 6100 VIS Cells for the WTW test set program Replacement lamp Accessories and options 8 Accessories and options 8.1 Accessories Description Model Order no.
photoLab® 6100 VIS Accessories and options 8.2 Test equipment Test equipment Description Model Order no. Test equipment for AQA1 PhotoCheck 14693 250 490 Test equipment for pipette volume PipeCheck 14692 250 498 Note Standard solutions for the WTW test set program are listed in the WTW catalog or on the Internet. 8.
photoLab® 6100 VIS Accessories and options 8.4 PC Connection cable: You can connect a PC to the photoLab® 6100 VIS in one of the following ways: Description USB printer – Cable with USB-B and USB-A plug Specialist shops – Zero modem cable 9-pin (D-sub socket) - 9-pin (D-sub socket) 820 070 You can connect a USB printer to the photoLab® 6100 VIS: Description – Cable with USB-B and USB-A plug Thermoprinter Order no. Order no.
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photoLab® 6100 VIS Appendix Appendix A.1 Menus Measuring (see section A.1.1) General settings and functions (see section A.1.2) A.1.1 Measuring z z z z z Concentration (see section A.1.1.1) Absorbance / % Transmission (see section A.1.1.2) Multi wavelengths Spectrum Kinetics A.1.1.1 Concentration Concentration – [Setup] – Dilution – Sample + distilled water – Sample blank value – User-defined blank value ( ) – Turbidity correction ( ) – – – – Display absorbance ( ) AQA (see section A.1.2.
photoLab® 6100 VIS Appendix Concentration – [Setup] – Edit method – [Next] – [Next] measure value pairs (Target value, Absorbance) – [Next] [Curve type] (Polygon line, Straight line, Parabola) [Meas. range] (Lower limit, Upper limit) – Enter formula – a5 ... a0, Meas. range (Lower limit, Upper limit) – Measurement data memory (see section A.1.2.
photoLab® 6100 VIS Appendix A.1.1.2 Absorbance / % Transmission Absorbance / % Transmission – [Setup] – AQA (see section A.1.2.2) – Measurement data memory (see section A.1.2.1) – [Wavelength] – Set new wavelength (nm) – [Transmission] <–> [Absorbance] – [Reference] – Reference absorbance A.1.1.3 Multi wavelengths Multi wavelengths – [Setup] – New method – Number, Name, Version, Citation form, Unit, Resolution, Cell – [Method list] – [All methods] <–> [Last used] – [Delete] – [Next] – Wavelength 1 ...
photoLab® 6100 VIS Appendix A.1.1.
photoLab® 6100 VIS Appendix A.1.1.5 Kinetics Kinetics – [Setup] – New profile – Edit profile – Scaling – Auto – Manual – Y-axis min – Y-axis max – [Profile] – [All profiles] <–> [Last used] – [Edit] – Slope – Compare spectrum – Subtract spectrum – [Open] A.1.2 General settings and functions z z z z ba75847e01 08/2009 [General setup] (see section A.1.2.1) [Logout] [AQA] (see section A.1.2.
photoLab® 6100 VIS Appendix A.1.2.1 General setup [General setup] – Language – Date/Time – Date – Time – Display settings – Contrast [%] – User management – [Setup] – Deactivate user management – Change password – [Add] – [Delete] – [Change] – Measured value memory – [Setup] – AutoStore ( ) – Filter – Mode – Absorbance / Transmission – Concentration – Multi wavelength – User – ID – Date – from ... to ...
photoLab® 6100 VIS Appendix [General setup] – Software/methods update – USB memory – PC – Cancel – Reset – Reset configuration – Delivery condition – Data transfer/Printer – Decimal separator for csv-Files – Point (12.34) – Comma (12,34) – Data format (print) – Short – Extended – Baudrate for printer (1200 ...
photoLab® 6100 VIS Appendix A.1.2.2 AQA [AQA] – AQA1 setup – Mode – AQA1 inactive – Weeks – Interval – Lock instrument (No/Yes) – Configuration of tests ... – PhotoCheck – CertiPUR UV-VIS 1 ... – AQA2 setup – Mode – AQA2 inactive – Weeks – Measurements – Lock methods (No/Yes) – Method...
photoLab® 6100 VIS Appendix A.2 Absorbance Analysis instructions AQA AQA labeling Logarithmic dimension for the absorption of the sample; negative decadal logarithm of the transmission. The exact proceeding to carry out the detection procedure is described in the analysis instructions. Analytical Quality Assurance. In the documentation, measured values are given an AQA labeling (AQA1 or AQA2), depending on whether or not the measurement was carried out with AQA and with which AQA level.
photoLab® 6100 VIS Appendix Correlation coefficient Detection procedure Kinetics MatrixCheck Specifies the extent of the linear relationship of value pairs when determining the zero point and slope for a user-defined method. The detection procedure designates the general principle of how a sample is brought into a form suitable for measurement. Different methods can be based on the same detection procedure. Measurement over a period of time. see AQA3.
photoLab® 6100 VIS Appendix Test set (test) A test set contains all reagents that are required for the photometric determination of the sample according to the analysis instructions. Transmission Part of the light that goes through the sample. Turbidity Light attenuation caused by diffuse scattering at undissolved substances. Zero adjustment Adjusting a photometer with a water-filled cell. A.
Appendix 180 photoLab® 6100 VIS ba75847e01 08/2009
photoLab® 6100 VIS 3.1 Index A Absorbance / % Transmission, measuring ................................................69 Accessories .............................................................................................165 Analysis timer ..........................................................................................100 Analytical quality assurance (AQA) .........................................................120 AQA ......................................................................
photoLab® 6100 VIS Menus ..................................................................................................... 169 Meter information .................................................................................... 145 Method ...................................................................................................... 77 Methods update ...................................................................................... 146 Multi-wavelengths methods ............................
photoLab® 6100 VIS U Update ....................................................................................................146 USB memory device .................................................................................22 User-defined methods Concentration ......................................................................................58 Multi wavelengths ................................................................................74 W Warm-up time ....................................
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