Owner's manual
Table Of Contents
- 1 Overview
- 2 Safety instructions
- 3 Commissioning
- 4 Operation
- 4.1 Switching on or off the photometer
- 4.2 General operating principles
- 4.3 Photometer settings and system administration
- 4.4 Zero adjustment
- 4.5 Measuring in Concentration mode
- 4.5.1 Measuring cell tests with barcode
- 4.5.2 Measuring reagent tests with AutoSelector
- 4.5.3 Measuring reagent-free tests and user-defined methods
- 4.5.4 Exceeding the upper or lower limits of the measuring range
- 4.5.5 Selecting a method manually
- 4.5.6 Settings for Concentration mode
- 4.5.7 Measuring diluted samples
- 4.5.8 Sample blank value
- 4.5.9 Reagent blank value
- 4.5.10 Automatic Turbidity correction
- 4.5.11 Programming / modifying user-defined methods
- 4.5.12 The IQ LabLink procedure
- 4.6 Measuring the Absorbance / % Transmission
- 4.7 Multi wavelengths methods
- 4.8 Spectrum
- 4.9 Kinetics
- 4.10 Timer
- 4.11 Memory
- 4.11.1 Overview
- 4.11.2 Instructions on using USB memory devices
- 4.11.3 Measurement datasets
- 4.11.4 Saving measurement datasets manually
- 4.11.5 Saving measurement datasets automatically
- 4.11.6 Displaying measurement data memory
- 4.11.7 Filtering measurement datasets
- 4.11.8 Inverting filters
- 4.11.9 Erasing stored measurement datasets
- 4.12 Copying files
- 4.13 Transmitting data
- 4.14 Analytical quality assurance (AQA)
- 4.15 User management
- 4.16 Reset
- 4.17 Photometer information ([Info])
- 4.18 Lamp counter
- 4.19 Software and methods update
- 5 Maintenance and cleaning
- 6 What to do if ...
- 7 Technical data
- 8 Accessories and options
- Appendix
Operation photoLab
®
6100 VIS
60
ba75847e01 08/2009
Determining the
calibration function
You have the following options to create a method:
z Measure and store:
Carry out a series of measurements with the following sample solutions
while at the same the photometer takes over the values:
– Blank sample to determine the reagent blank value
(with deionized water instead of sample, see section 4.5.9)
– at least one, up to ten standard solutions in different concentrations.
The photometer stores the nominal value/absorbance value pairs of the
individual measurements and determines the resultant characteristics of
the calibration. When doing so, you can select the following line types:
Polygon line, Straight line or Parabola.
z Enter as value pairs:
Entry of the value pairs, Nominal value (concentration) / Measured
absorbance of an already available
test series with the following sample
solutions:
– Blank sample to determine the reagent blank value
(with deionized water instead of sample, see section 4.5.9)
– at least one, up to ten standard solutions in different concentrations.
Based on the entered value pairs, the photometer determines the
characteristics for the calibration. When doing so, you can select the
following line types: Polygon line, Straight line or Parabola.
z Enter a function:
Entry of a function to calculate the concentration from the absorbance
(reverse calibration function). You can enter on the photometer the
coefficients of a polynomial equation of the following type:
c = a0 + a1·A + a2·A
2
+ a3·A
3
+ a4·A
4
+ a5·A
5
Note
Entering the formula is especially simple if you measure with a commercial
test set for which the manufacturer has given the value for the coefficient a1.
It is often called the "Factor" and corresponds to the reciprocal value of the
slope of the straight line of the calibration function.
If a linear
function (straight line) should be entered, it is necessary to enter
the coefficients a0 and a1 to receive correct measured values.
with:
c Measurement result, e.g. concentration
a0 to a5 Coefficients (input range 0.000 to 1000,000)
A Absorbance