Owner's manual
Table Of Contents
- 1 Overview
- 2 Safety instructions
- 3 Commissioning
- 4 Operation
- 4.1 Switching on or off the photometer
- 4.2 General operating principles
- 4.3 Photometer settings and system administration
- 4.4 Zero adjustment
- 4.5 Measuring in Concentration mode
- 4.5.1 Measuring cell tests with barcode
- 4.5.2 Measuring reagent tests with AutoSelector
- 4.5.3 Measuring reagent-free tests and user-defined methods
- 4.5.4 Exceeding the upper or lower limits of the measuring range
- 4.5.5 Selecting a method manually
- 4.5.6 Settings for Concentration mode
- 4.5.7 Measuring diluted samples
- 4.5.8 Sample blank value
- 4.5.9 Reagent blank value
- 4.5.10 Automatic Turbidity correction
- 4.5.11 Programming / modifying user-defined methods
- 4.5.12 The IQ LabLink procedure
- 4.6 Measuring the Absorbance / % Transmission
- 4.7 Multi wavelengths methods
- 4.8 Spectrum
- 4.9 Kinetics
- 4.10 Timer
- 4.11 Memory
- 4.11.1 Overview
- 4.11.2 Instructions on using USB memory devices
- 4.11.3 Measurement datasets
- 4.11.4 Saving measurement datasets manually
- 4.11.5 Saving measurement datasets automatically
- 4.11.6 Displaying measurement data memory
- 4.11.7 Filtering measurement datasets
- 4.11.8 Inverting filters
- 4.11.9 Erasing stored measurement datasets
- 4.12 Copying files
- 4.13 Transmitting data
- 4.14 Analytical quality assurance (AQA)
- 4.15 User management
- 4.16 Reset
- 4.17 Photometer information ([Info])
- 4.18 Lamp counter
- 4.19 Software and methods update
- 5 Maintenance and cleaning
- 6 What to do if ...
- 7 Technical data
- 8 Accessories and options
- Appendix
photoLab
®
6100 VIS Operation
73
ba75847e01 08/2009
4.7 Multi wavelengths methods
4.7.1 Basic information on Multi wavelengths measurements
The multi wavelength function of the photoLab
®
6100 VIS enables free
calculation of any parameters from the absorbance values of several
wavelengths. When doing so, two to ten wavelengths can be used.
Calculation takes place based on the following equation:
The wavelengths 1 to 10 can be freely selected within the measuring range.
Example: Protein
determination
according to
Warburg-Christian
Protein determination according to Warburg-Christian is based on
measurement of the optical density (=absorbance) at 260 and 280 nm in a
10 mm cell. From this the protein content is calculated as follows:
To convert the basic equation (1) into equation (2), the coefficients are set to
the following values:
All absorbances are measured in one step and the result is calculated
according to the equation.
with:
R Result
a0 to a10; b0 to b10 Freely selectable coefficients
(entry range 0.000 to 1000,000)
A1 to A10 Measured absorbance at the individual
wavelengths 1 to 10
(equation 1)
1010...22110
1010...22110
AbAbAbb
AaAaAaa
R
⋅++⋅+⋅+
⋅++⋅+⋅+
=
a0 =0 b0 =1
a1 =1,55 b1 =0 a1 = A(280 nm)
A2 = -0,76 b2 =0 A2 = A(260 nm)
A3 =0 b3 =0 A3 = Not used
A4 =0 b4 =0 A4 = Not used
a5 =0 b5 =0 a5 = Not used
a6 =0 b6 =0 a6 = Not used
a7 =0 b7 =0 a7 = Not used
a8 =0 b8 =0 a8 = Not used
a9 =0 b9 =0 a9 = Not used
a10 =0 b10 =0 a10 = Not used
(equation 2)
)nm260(A76,0)nm280(A55,1]ml/mg[c
Protein
⋅−⋅=