User Manual BOD ANALYST PRO® Software for BOD Testing Easy • Efficient • Accurate ® ® ® Windows XP • Windows Vista • Windows 7
Page Intentionally Left Blank YSI BOD Analyst Pro® User Manual ii
CONTENTS SECTION 1 1.1 1.2 1.3 INSTALLING YSI BOD ANALYST SOFTWARE ON YOUR PC ............................................................ 5 COMMUNICATING WITH THE YSI DO INSTRUMENTS ................................................................... 12 QUICK START GUIDE ................................................................................................................... 14 SECTION 2 2.1 2.2 2.3 2.4 2.5 2.6 TAKING INITIAL DO READINGS .........................................................
SECTION 8 8.1 8.2 8.3 8.4 KEEPING YOUR DATA SAFE ..................................................................................... 65 DATE ........................................................................................................................................... 65 HARDCOPIES ................................................................................................................................ 65 UNDERSTANDING DATA FILES ........................................................
SECTION 1 INTRODUCTION BOD Analyst Pro® software is PC Windows-based application software designed for use with YSI dissolved oxygen instrument systems. Using a serial interface the user defines parameters in the BOD Analyst Pro software that establishes two-way communication between instrument and PC.
The installation program will display the following screen. Please wait momentarily for the computer to initialize the installation. The installation program prompts you to follow instructions on the screen to complete the installation. The next screen will display the locations of the default directories. Click Next >> to accept these locations, or use the Browse… icon to change locations if you prefer. After clicking on Next >> the first of two license agreements will appear.
The next screen will indicate what is about to be installed. Click Next >> to formally start the installation. A status screen will appear next as shown below. The installation process may take a few minutes depending on the speed of your PC. Once installation is complete a confirmation screen appears to verify completion. Both progress and completion screens are shown below.
To complete the installation process you will be prompted to restart your computer. Click Restart or one of the other options before running the BOD Analyst Pro software.
A BOD Analyst Pro icon will appear on your Desktop upon restarting your computer. Double click the icon to start the program. Alternatively, you may use the Window’s Start icon in the bottom tray. BOD Analyst Pro will be listed in the Programs menu. IMPORTANT: YSI BOD Analyst Pro requires installation of .NET Framework version 3.5 or lower on your PC in order to run properly. The .
1.1.1 STARTING THE PROGRAM Double click on the Desktop BOD Analyst Pro software icon that was set up in the installation program. BOD Analyst Pro You should see the BOD Analyst Pro software Bench Sheet screen (Figure 1). This is the main screen from which all functions will be accessed and all results and most information will appear. Figure 1.
Submenu Displays File | New | Open | Archive | Print Bench Sheet | Exit | Read* | Initial | Final | Retake Initial | Retake Final | Explain | Setup | Samples | Groups | Criteria | General | YSI Instrument | Facility | Communication Setup | Serial Numbers | Trending | Graph | Help | Search Help | About | *Initial, Final, and Retakes should not be active when reviewing data on dates other than pre-assigned “read dates”. Figure 2.
1. 2 COMMUNICATING WITH THE YSI DO INSTRUMENTS YSI BOD Analyst Pro software was designed to directly communicate with each of the following instruments: YSI 52, 5000, 5100, ProODO®, Professional Plus® or the MultiLab instrument. You will need an available Comm port or USB port on your PC and the appropriate serial cable or USB cable to connect PC to instrument. Once your connection is complete the BOD Analyst Pro software can test and verify proper communication setup.
a 4010-2 or 4010-3 make sure the OBOD probe is connected to channel 1, see instrument manual. 5. Click Test Instrument Communication. A successful connection will return a message showing relevant data or information from the instrument. If the connection is not properly set an error code will return with possible causes listed. Refer to figures below showing the communication screens. In the first example a ProODO system was set up successfully communicating via PC port COM5.
1.3 QUICK START GUIDE Once you install BOD Analyst Pro on your PC, make connections and communicate properly with your DO instrument, you may find the steps below useful to quickly setup and run a batch of samples. These steps are listed mainly to introduce you to the program. Please read the entire user manual for better understanding of the many features available for your BOD application. 1. Verify that the Data Directory (path) is correct. The default directory path is C:\BOD\DATA\DATA.
□ TIP – OFF/ON If box checked the parameter is turned ON. Blank is OFF. General Settings (Setup / General) TIP – Tip Strips provide information related to each setting.
Serial Number Setup (Setup / Serial Number) Serial numbers give each bottle a unique ID; in the batch and over time.
4. Set up Samples. By default BOD Analyst Pro has three entries in the Sample Setup. They are DilBlank (dilution blank), Seed, and Standard (e.g., GGA Standard). Open each sample field to gain familiarity. Locate the bottom left field and change the Standard Sample from “No Seeding” to “Seed” and enter “2” for ml of seed to each bottle. Next create two new samples using the Add New icon. Name one Influent and configure 3 dilutions (bottles) at 8, 10, and 12 milliliters.
5. Set up a Batch (Build Batch). From the Bench Sheet screen click on the Calendar icon, select today’s date, and confirm by clicking Use This Date. The date will appear in the Sample Date field. Now click on the Build Batch icon near the center of the screen. The Build Batch appears without entries in the bench sheet table. However, the defined Samples will appear in the field labeled Choose Samples. Many samples can be listed here but in the quick start example just five (5) samples were configured.
You may enter bottle numbers now by clicking on the appropriate field in the table. Type or scan in the number. You may also enter bottle numbers when you take Initial DO Readings. Bottle numbers may be scanned in with a bar code reader. Refer to Section 10 for details. At this point you may add general notes related to your batch under Notes, or notes relevant to specific bottles by clicking on the Sample Name of the bottle and entering the note in the Bottle Notes field.
To begin Initial DO readings click Read from the top line menu and then Initial. Take Readings Status displays guide you through the readings prompting you to make sure the BOD probe is in the bottle and using criteria you set up for DO reading stability. Temperature is recorded and monitored by the software to be within limits set under YSI Instrument setup.
7. Read Finals. Five days later restart BOD Analyst Pro software. Friday 07/01/2010 would appear as Current Date rather than the simulated 6/28 in this example. Select the Sample Date to load the batch. The Sample Date is the date that the Initials were read, not the current date. Incubation would read 6/28/2010 to 7/1/2010 in an actual 5-day run. Select Read from the top line menu and then Finals from the Main Menu. Follow the prompts on the YSI or the PC to read the Day-5 readings.
SECTION 2 SAMPLES 2.1 WHAT IS A "SAMPLE?" With YSI BOD Analyst Pro software you can organize your work by sample. A sample is anything for which you want to know the BOD. Samples for BOD determination can be taken from various points in your wastewater treatment system or environmental water.
QC Settings – as required set limits on a sample for alerting operator when out of range for Final BODs Now refer to the Sample Setup Form below and follow the set up of a sample named Effluent. The example Sample Setup above shows a sample named Effluent, with specific sampling information identified under Description. There are 3 dilutions containing 75 ml, 100 ml, and 150 ml of sample. This would be 25%, 33% and 50% sample, respectively. “Note: Dilutions are in ml” is a message in the setup box.
2.2 USING SAMPLE SETUP The Sample Setup Form is used to add new Samples to the database or to change old ones. The Sample Setup Form is accessed from the top level menu (Menu) of the Bench Sheet by selecting Setup / Samples. Sample Setup information is used as originally configured when adding a sample to a day’s batch. For example, you set up Effluent with three dilutions of 75, 100, and 150 ml with 8 ml of seed.
Dilution Setup From Setup / General you choose the units of the dilutions. You may select ml or %. Once selected and confirmed on the General Setup Form by clicking OK, the message on the Sample Setup Form will “note” which units are in use. See Sample Form above. If you change dilution units on the General Setup Form this will affect your sample dilutions previously entered. The numbers will not change so the BOD calculations may not reflect what you intend.
Dilution Blank If checked this is a dilution blank test. Only the depletion is calculated for these tests. There is no BOD value calculated. The Maximum Dilution Blank Oxygen Depletion criterion is applied to Dilution Blank Tests. By default this is 0.2 mg/L, but may be changed by the user. Used as Seed If checked the sample can be used as a seed and will therefore appear in the Seed list for each Sample.
report of a Batch that includes a BOD of the "clarifier," the Sample Name would be reported as "secondary." Therefore, if you edit the name take care to choose a Sample Name that accurately describes the Sample as defined so not to cause confusion with the new name. 2.5 NAMING SAMPLES When determining BODs by hand with paper forms many people develop brief abbreviations for their samples to save time.
2.6 DELETING A SAMPLE To delete a sample use the tool bar buttons to locate the sample (e.g., Effluent) and click on the Delete button (>X). A pop-up confirmation message will appear. If you click OK the sample will be permanently deleted from the list of samples. To create this sample again you will need to reconfigure it with the Add New (>*) function and re-enter information. When a sample is deleted from the current list of samples the record of its use in the database is maintained.
SECTION 3 SEEDING SAMPLES 3.1 WHY SEED? Seeding is necessary when there are insufficient microbes present in the sample to adequately deplete oxygen during the course of the test. Adding small amounts of microbes promotes the depletion of oxygen to allow accurate quantification of the oxygen depletion. Different samples need different levels of seed. Seeding is common with an effluent sample that has been chlorinated.
Sometimes the seed material is taken from a sample that is already being tested for BOD. As shown above an influent sample is commonly used since microbes are present in the raw sewage. Above Influent was assigned in the Seed Control Sample Name field. In order for Influent to be in the Seed list above you will need to check the box for “Use as Seed” during Sample Setup of the Influent sample. See the example to the right.
If you decide at some point that it would be best to change your seeding method, you can change the seeding method on the Seed added to field on the General Setup Form. However, you must adjust the seed quantity for each of your seeded samples to reflect the new method. The reason is that the units for the seed quantity are different for the two methods (ml of seed material in each bottle vs. ml/L of seed material in dilution water).
SECTION 4 SAMPLE GROUPS 4.1 WHAT IS A "SAMPLE GROUP"? A Sample Group is a predefined, stored set of samples available for fast and easy setup of a Batch. The Sample Group (or Group for short) is intended for batches that are frequently used to set up a specific BOD test. For example, assume that every Thursday you set up and run Initials for a BOD test batch that includes the same samples, same dilutions, same seed, and other relevant information.
4.3 ADDING A GROUP AND CHOOSING SAMPLES From the Group Setup form, click the Add New button. A blank form appears (see above). Enter the Group Name (Thursday), select names from the Choose Samples field and move each to the Samples in Group field. All available samples will be listed. Add Samples by either double clicking on individual names under Choose Samples, or use the >> icon to enter all sample names at once.
SECTION 5 BATCHES 5.1 WHAT IS A "BATCH?" A group of samples set up and read on a specific date is referred to as a Batch. The Batch is identified by all of the samples for which the Initial DOs were read on that date. In the BOD Analyst Pro software the Sample Date is used to locate a specific batch. If only the Initial DOs have been read the batch is sometimes referred to as an Open Batch.
You may search by Search Date icon if you know the date of a Batch or you may use the Open Batch icon to display a list of Open [Batches]. In addition, under Open, you may check the All list to find dates for Open and completed batches in the records. 5.2 LOADING A BATCH To load a batch into the Bench Sheet enter a date into the Sample Date field (see above). If a batch for the day has been started, it will be retrieved into the Bench Sheet.
An example of the 07/11/2010 Sunday Batch has been opened. The Initials have been read and the screen should appear similar to the example below. Note that the Sample Date field is filled in as 07/11/2010 and the top field under Incubation Period is also filled in with the date Initials were read. There are 5 Samples in the Batch with a total of 12 bottles when you count the dilutions that had been previously configured under Sample Setup.
5.3 THE BUILD BATCH FORM The Build Batch Form appears when you click on the Build Batch icon on the Bench Sheet screen (main screen). You will need to assign a date and enter it into the Sample Date field. Typically that would be the current date if you were building a batch for which Initial DOs will be read on the same day. The build batch form is used to specify the samples and the appropriate dilutions for the batch.
The table on the Build Batch form describes the batch. Each row in the table is a Sample. Sample Names appear in the first column. There are 5 columns for Sample dilutions and 2 columns for Seed definition. Use the mouse or the arrow keys to move through the table. You typically define Samples prior to building a batch. With a click or two of the mouse you can usually fill out a Batch Table. If you need to change entries use your mouse or keyboard arrow keys to move to the cell and make the change.
remove one Sample at a time as described in 5.4.2 or use the Delete button on the Open Batch list. 5.4.4 ADDING MORE DILUTIONS AND CHANGING SEED CONDITIONS If you build your Batch from preconfigured Samples and decide that it may be useful to add additional dilutions (5 total per sample), you can click on the cell in the table and type in the desired sample volume (“dilution”). The value would be ml sample or % sample depending on your setup.
The example below shows a step in the change from a seed sample simply named Seed to a predefined sample named Influent. Influent was designated for “use as seed” when it was set up as a sample. At the same time you change the Seed Sample you may change the Seed Quantity. In this example the number 3 was typed in to replace the number 2 so the revised Seed is Influent and the number of ml per bottle in the Effluent sample is 3. Above: Changing Seed after the original batch was defined.
As before the pop-up message warning that “previous data will be replaced” occurs, but once this is confirmed with OK the new Bench Sheet appears with the changes shown. In this example there will now be 14 (versus 13) total bottles since an additional dilution was added to Effluent. Next Bench Sheets will be discussed in more detail. 5.5 THE BENCH SHEET The Bench Sheet is the main form of the BOD Analyst Pro software.
Data may be entered from the keyboard in a spreadsheet fashion or entered via direct serial communication with a YSI DO Instrument. Information and data to enter include the bottle number, amount of sample, amount of seed used, initial dissolved oxygen reading and final dissolved oxygen reading. For each sample dilution bottle the oxygen depletion, seed correction factor and BOD are calculated automatically. One exception is the Dilution Blank, for which only oxygen depletion is calculated.
5.6 READING INITIAL DO’S Once you build the Batch and load it into the Bench Sheet select Read / Initial from the Main Menu. This connects the BOD Analyst Pro program to the YSI DO instrument assuming that communications testing has been verified. A pop-up window like the one displayed here will lead you through the prompts to take Initial DO readings. With the YSI 52, 5000, and 5100 instruments you Accept/Confirm the question or entry from either the instrument keys or the PC mouse or keyboard.
The YSI 5100 system accommodates a serial barcode reader (optional accessory) that may be useful to speed the entry of bottle numbers. The bottle number entry appears both on the YSI 5100 display and in the Take Readings window shown above. You may consider using a commercially available barcode reader that connects to your computer via 9-pin serial or USB serial port.
Enter the Sample Volume (ml) and the Seed volume (ml). Confirm with an Enter or Click to another field. Now click Apply Changes (lower right of Bench Sheet). The new Bench Sheet will reflect the addition of the new bottle (dilution) for that sample (Effluent in the above example). If you would like to add a duplicate dilution (same sample volume) to one already in the Sample you may use New Bottle to perform this addition. 5.
The Bench Sheet should now show the additional bottles under this added Sample Name (e.g., Above Outfall). Proceed with entering bottle numbers and/or reading Initial DOs. When you have completed Read Initial click Open Batch and then click on the appropriate date that applies to your Batch. Confirm that you want to save the data changes. The revised, updated Bench Sheet will now appear on your screen.
NOTE: In the example above both the added bottles and the added sample are shown in the Bench Sheet table. So, after entering a Group defined with 13 bottles you end up with 16 bottles due to changes you implemented during the Initial Read operation. 5.9 MARKING A BOTTLE Use the Manual Mark icon to mark rows in the Bench Sheet table that are independent of the criteria that are set. These are choices you make for whatever reason.
Enter the sample date of interest. When the sample date is entered and you click on the Search Date icon, any previous Batch from that date loads into the Bench Sheet on the screen. Incubation Period This is the date range of the BOD incubation period. The start date of the incubation period is set when Initial DOs are read. The end date is set when finals are read. Seed Correction The Seed Correction field shows the calculated seed correction in mg/l DO per ml of seed used.
Seed Volume (ml) This is the seed volume in milliliters introduced into BOD bottles that use seed. If the field is left blank the seed volume defaults to zero. Initial DO (mg/l) This is the measured dissolved oxygen at the beginning of the incubation period expressed in milligrams per liter. Final DO (mg/l) This is the measured dissolved oxygen at the end of the incubation period expressed in milligrams per liter.
Note: This does not affect the original Build Batch table or Group Setup form. SECTION 6 TAKING INITIAL DO READINGS 6.1 ENTERING BOTTLE NUMBERS You may enter bottle numbers in a Batch in several ways. For small batches, especially less than 15 to 20 bottles where no special characters are needed, manual entry by the keypad into the Bench Sheet is simple and fast. When the batch size is large and/or you use barcode labeling then a barcode reader is practical and likely to improve accuracy.
Move through the bottle number assignment prompts and accept/confirm after entering or reading information. You may reject or skip bottles. The order in which they are read is up to you. Retakes and correcting errors will be covered elsewhere in the manual. Below is an example of the sample named “Effluent”. It includes three dilutions and bottle numbers will be assigned as the bottles are read. The top line of the display will show the Sample name and dilution of the next bottle to be read.
numbers and DO readings will be added to the Bench Sheet. Below is an example of this screen about halfway through a Batch. Bottle numbers were entered by typing in this example. You can also move between dilutions (bottles) without going through the batch sequentially. The SKIP, UP, and DOWN buttons can be used to navigate through the batch. SKIP Moves to the next bottle (dilution).
When you finish the bottles in a Batch the program will display an “Initial Read Complete” message on the YSI 5100 display and the Bench Sheet shows the completed readings. 6.1.2 USING THE BARCODE READER The YSI 5100 is the only DO instrument designed to accommodate a barcode reader. YSI offers the barcode reader as an optional accessory to the user. You may be able to use a commercially available barcode reader that connects directly to your PC by serial connection.
NOTE: If using the YSI 5100 with barcode reader be certain that the codes are no more than 3 characters long so that they will fully display on the 5100 instrument display. You may also enter some bottle numbers with a barcode reader and some manually (keyboard entry from PC or 5000/5100 instrument keypad). The program allows this in the event that some bottles in a batch may lack barcodes or a code label is physically compromised. 6.1.
A second technique is Dual. Set this parameter as above under YSI Instrument Parameters. When you are reading DO you must both wait for stability criteria to be met and press CONFIRM. The order is not relevant, but both actions are required to enter and store readings. If the reading is stable before you press CONFIRM then you may see that the DO reading is stable based on instrument signals (e.g., beep).
The default Supersaturation setting is 9.09 mg/L. You may change it if you like. Often altitude would be a reason to change this. It is not uncommon to collect a sample in cold weather that is over 10 mg/L. While a 10 mg/L sample may not be supersaturated at low temperatures it could be at 20 C. If such a sample is placed in the incubator it may “outgas” and cause erroneously high BOD. The supersaturation alarm will warn you that the reading is above the value that you have specified.
In this example, one Influent bottle has been read. There are three total in the Batch. There tends to be less experimental error if you measure the dilution with the greatest sample volume, in this case Bottle #111, In order to perform this operation you must enter the bottle numbers of each sample that precedes the sample that will be read. Therefore you would enter the B# for 109, press SKIP, then enter B# for 110, press SKIP, and then B#11 where you would proceed to CONFIRM the reading of this bottle.
bottle #109 is the selected bottle.
SECTION 7 TAKING FINAL DO READINGS 7.1 SELECTING A BATCH From the main menu (Bench Sheet) window enter the date of the batch for which you would like to take readings. Enter this date in the field Sample Date. Sample Date is the date which you started the BOD test incubation period, hence the date you read Initial DOs. Alternatively you may click the Open Batch button to select a batch that is not yet complete.
The July 16 batch will appear as shown below. This example of an Open Batch containing 13 bottles will be ready for Final readings 5 days from its setup, or on July 21. 7.2 TAKING A FINAL READING The example above assumes that you are using a YSI 5100 at COM4 to take readings. Below are instructions for using the 5100 DO meter. There will be prompts at both the YSI 5100 display and simultaneously at the BOD Analyst Pro display.
Effluent 100.00 Bot #11 Ready? If the bottle is ready then press either the YSI 5100 keypad or the program display. The ACCEPT/CONFIRM button on the keypad or program window “confirms” you are ready for the reading to begin. Once the reading begins there are stabilization criteria set up under YSI Instrument Parameters that determine when a stable value has been achieved (see Section 10 under Criteria in the user manual). During the read operation the displays take on a new appearance.
Auto: The program automatically stores the DO reading when it meets DO stability criteria (green light illuminates). The display prompts move on to the next bottle on the Bench Sheet list. Dual: When the DO reading meets stability criteria (green illumination) press CONFIRM to store the reading. Manual: Press CONFIRM to store the currently displayed DO reading from the YSI DO instrument. If using the YSI 5000/5100 the DO instrument display should display STABL.
The Bench Sheet related to Criteria is discussed in more in Section 10, Customizing Your Program. A few explanations will be pointed out here to understand the Final Batch for this test. There are no BODs calculated for Dilution Blank. The DO depletions have limits and the BOD has no particular significance or need in other calculations. The Standard was performed for this example with one seed prep named Seed and identified by the number (6) in the Seed Corrections field.
7.3 CORRECTING MISTAKES If you need to correct a Final DO reading that was stored you can retake Final DO readings just as you were able to retake Initial DOs. The program allows you to correct the Final DO in the Bench Sheet providing that the incubation period is between 4 and 7 days. To do this after completion of Finals load the “read” batch into the Bench Sheet and select Read / Retake Final. Select to read All Bottles or Select This Bottle.
SECTION 8 KEEPING YOUR DATA SAFE 8.1 DATE The BOD Analyst Pro software depends on the correct date to do calculations and manage the data. The current date on your computer is displayed in the main menu. It is located in the field on the right labeled Current Date. If the date is not correct exit BOD Analyst Pro software and correct the date. In Windows XP click Start / Settings / Control Panel and find the Date/Time icon in the Control Panel.
during installation. Check the upper left corner of the main screen (Bench Sheet) for the field labeled Data Directory if you are not sure the path of your database file. See the example below. BOD Analyst Pro software allows you to have any number of databases, but the program allows you to open only one at a time. There are reasons you would need multiple databases.
To open an existing database file select File / Open to begin with the following window. Click on the Browse icon shown by the arrow in the field. A window labeled Open will appear with directory path and filename name information. Click on the name of the file you want to open and then click OK to confirm your choice. The Data Directory field will reflect this change when you return to it. BOD Analyst Pro software data files can be located anywhere (i.e. the local drive, thumb drive, network drive, etc.
8.4 BACKUP You may also want to save your data.mdb file periodically on a portable memory device like a thumb drive or portable hard drive. Printing a Bench sheet every time you finish a run of initials or finals will insure that you will always have at least the raw data from which you could do the calculations by hand. However, a computer failure could also destroy your configuration information (your Samples’, Group, Criteria, and so on).
SECTION 9 GRAPHING 9.1 INTRODUCTION The graphing function provides you the ability to produce time-series type graphs (data vs. time). Up to two samples may be displayed on the same plot. The X-axis is time (date) and the Y-axis may be one or two variables in the database. The Y-axis units are mg/l since the variables are the Final BODs of Samples selected for time-series analysis. Use Trending in the main menu but first make sure you have the correct database ready to load.
file. You may choose one or two Samples to compare. The length of time may be days to years depending on the size of the database. Finally there is a field to label the title of your graph or plot. You may type in up to 80 characters/spaces to describe your graph. Below is an example graph displayed for reference to accompany the setup information that follows in the next sub-section where you will learn to set up and print a graph. 9.
There are icons that function to size and position the plot to fit your needs. The use of these icons is very intuitive however some discussion of their use may help you get started. First click on the “magnifying glass” icon to open up a group of 6 more icons. These are listed in the table below. Try using each on a practice graph. In this example plot the Y-values are >165 and <230 so the Define plot area tool worked well to “box in” in the data to better fill the screen.
The graph now compares two parameters over the same time period. The Blue line (top) is the Standard and the red line (bottom) is the Raw Sewage or influent in the wastewater treatment plant. There are limitations to the Graph/Plot functions. First is that only two samples may be compared at one time. Second is that the axis is not scaled for individual plots so comparing very small yvalues with very large y-values between two samples will limit resolution in the small values.
size and orientation. Below is the printed graph. Note that the graph adds Facility information that is set up under main menu Setup / Facility. Title, along with legends and axes labels, appear along with date/time stamps. The graph/plot features work well to take a quick look at a database file to compare one or two parameters over time. For example the effluent as a single parameter would be useful to follow over time. Some of the seasonal fluctuations can be interesting.
When you click on the Export Data icon the Choose File Name screen appears as shown to the right. Note the file format is “.csv” which imports well into common spreadsheet files like Microsoft Excel. Type in a name but preserve the extension .csv and assign a path for finding the file. Save by clicking OK. The data you selected will be in the csv file. The resulting spreadsheet (no formatting) will appear similar to the figure below.
From the spreadsheet data you can format data to make reports or tables that suit your needs. In addition you may use the plotting functions of the spreadsheet application program to plot or chart the data. And the imported data can be used to check a variety of statistical information using Microsoft Excel’s simple to use mathematical functions. 9.
As mentioned above if you need additional statistics functions for analysis consider exporting sample data as “csv” files via the Export Data feature and use the many statistical functions available in many spreadsheet application programs.
SECTION 10 CUSTOMIZING YOUR PROGRAM The BOD Analyst Pro software has significant flexibility to cover the wide variety of ways that people measure and analyze BODs. Some features are simple and a matter of personal preference. Other features may be important in regulatory reporting. This section describes the advanced settings that you can use and specify in YSI BOD Analyst Pro software. You make your choices on several forms under the Setup menu. 10.
After storing a reading to one bottle be sure that you move the DO probe to the next bottle immediately. Of course, use your lab protocol for cleaning the probe between bottles as prescribed. The advantage to moving the bottle right after a reading is that the probe can be equilibrating to the next solution while you are entering the bottle number. The Auto stabilization routine starts immediately after the bottle number is entered. You can choose exactly how and when a stable reading will be stored.
Below are brief descriptions of each criterion listed in the Criteria Setup form shown above. Maximum Dilution Blank Oxygen Depletion (mg/l) This is the maximum acceptable value for Initial DO minus Final DO in the dilution-blank test. This criterion is checked only for Dilution Blank samples. The default value is 0.2 mg/l. Maximum Initial Dissolved Oxygen (mg/l) This is the maximum acceptable value for the Initial DO. This is checked for all tests. The default value is 9.7 mg/l.
The percentage depletion is defined as Final DO / Initial DO * 100. This criterion is checked only for samples that are used as Seed. The default value is defined by the limit 60%. To turn off the test enter 100% for Maximum. Use Seed that was closest to 50% depletion This is one alternative to the 40% to 60% Seed Test. For the entire Seed sample find the bottle (dilution) that is closest to 50% depletion. Mark all other bottles in the sample.
NOTE: Criteria may be changed anytime after the test is complete and results are shown. Use Setup / Criteria to access the form and then OK to confirm. Click Manual Check and new criteria results will be displayed for the batch.
10.3.1OPTIONS WHEN SEED SAMPLES DO NOT MEET “TAKE 2 – LEAVE 1” If you encounter a situation where none of the seed samples in your setup meet the ‘Take 2 Leave 1’ rule and therefore are not used in the BOD calculation, there are three options in BOD Analyst Pro® desktop that may be helpful. OPTION 1 In this scenario assume you have checked the box to “Apply take 2 leave 1 rule” in Criteria setup. In the Bench Sheet the seed samples will be reported using default values (2.000 or 1.
In Option 1 the Bench Sheet line remains unmarked (no highlight), “2.000” is entered for O2 depletions, and “invalid” BOD values of 66.67, 50.00, and 40.00 mg/l are reported (2 mg/l divided by % dilution). Note: the red highlights partially obscure the BOD readings in the figure. OPTION 2 The same criteria from Option 1 are applied. However after the completed Bench Sheet appears, re-enter the Setup/Criteria form and “uncheck” the box for the Take 2 Leave 1 Rule (see below).
values would be visible for the seed samples, but no seed corrections would be applied to any sample to which seed was added. In the figure above (just above the data table) note the comment “No seed correction”. In the above example BODs of 63.00, 47.50, and 31.40 mg/l were listed in the BOD column for Seed Samples (top to bottom). However, under the Notes column (far right) “D, SD” was reported and defined at the bottom of the Bench Sheet.
As seen in the figure above the 3 seed sample lines are no longer marked, the BOD values are all calculated, and the average of the 3 values is reported as the Final BOD (47 mg/l in the example). This option includes notes UM for seed samples and S for samples used with seed. The UM identifies that the user “manually unmarked” the seed samples. The S identifies that samples using seed were calculated with the Seed Correction Factor that was out of range.
10.4 THE GENERAL SETTINGS FORM The General Settings form is used for a variety of BOD Analyst Pro settings that are not considered measurement criteria per se. Access the form from main menu using Setup / General. The form is divided into three fields. Each setting and the options are discussed below. Normalization Factors Normalization factors allow you to set the days for your Final DOs readings. The program multiplies all depletions by the normalization factor for the day of the Final DO.
the measured dilutions to derive the others. The default value is unchecked (OFF). Blank compensation You may compensate your BODs for the depletion of your blanks by checking this field. The Blank DO depletion (albeit small) will be subtracted during the BOD calculations. This is common in some countries outside the US (e.g., UK). You can change this field at any time. The default setting is unchecked (OFF).
10.5 THE YSI PARAMETERS FORM This form works with your YSI DO instrument to establish stability criteria, alarm conditions, and options related to reading bottles. The program was initially designed for use with the YSI 5000/5100 but the settings apply to all YSI DO instruments used with BOD Analyst Pro. You can change the settings on this form. The default settings are shown in the figure below. Technique This field describes the conditions that define a stable reading. If met the reading will be stored.
Stabilize timeout This time in seconds is the max time the program will wait for the reading to become stable. If the reading has not met stability criteria by this time, the program will store the reading anyway. Legal values are 5 through 500. The default value is 300. Sample hold time This is the hold time in seconds for Samples other than blanks. The hold time in combination with Sample change is one criterion related to stability settings.
Final Low Temp Limit If the temperature of your final DO is below this temperature (C) the program will display a warning message in the Take Readings window and (if using the YSI 5000/5100) on the instrument display. You must ACCEPT/CONFIRM the condition before the reading is stored. Legal values are 0 through 99.9. The default value is 18.
Instrument Type Select the DO instrument with which BOD Analyst Pro will communicate. The list includes the YSI 5000, YSI 5100, YSI 52, YSI Pro, and YSI ProODO. All of the communication parameters (baud rate, data bits, parity, stop bits, flow control, and timeout) are programmed into the software for the specific instrument type you selected. You can not change these parameters. The ProODO is shown above. The default instrument is the 5100.
10.7 SERIAL NUMBERS Serial Numbers can be used to uniquely define a particular BOD test bottle or sample. It is important to understand the difference between Samples and Serial Numbers. A typical sample may be called Effluent with dilutions of 100, 150 and 200 ml, and 3 ml of seed added to each bottle. Suppose a laboratory is testing the Effluent Sample one day and the program assigns a serial number of 328. Normally the Serial Numbers increment, therefore the next sample would be number 329.
Last Serial Number This field displays the Last Serial Number that was assigned by the software. This field is read only, as shown by the shaded box. The Serial Number will be displayed when reading the Initial DO and Final DO on the computer. When the Sample Name of the specific bottle is highlighted as shown in the figure, the Serial Number will be displayed in the lower left field labeled Serial Number.
SECTION 11 CONTACT INFORMATION For questions regarding BOD Analyst Pro® software, or any YSI equipment, please contact YSI Technical Support at (800) 897-4151 or e-mail at environmental@ysi.com. For ordering YSI equipment or accessories please see your YSI dealer or use the contact information below. YSI, Incorporated 1725 Brannum Lane Yellow Springs, Ohio 45387 USA (800) 897-4151 USA Only (937) 767-7241 FAX: (937) 767-9353 Website: www.YSI.com E-mail: environmental@ysi.
SECTION 12 ACCESSORIES INSTRUMENTS 5000-115 Oxygen Instrument, Digital, Lab-115V 5100-115 Oxygen Instrument, Digital, Lab with SOUR software – 115V Professional Plus Instrument (comes with Procomm II Communications Saddle and USB) ProODO Optical Oxygen Instrument (comes with Procomm II Communications Saddle and USB) 4010-1, single channel MulitLab instrument 4010-2, dual channel MultiLab instrument 4010-3, three channel MultiLab instrument PROBES 5010 BOD Probe, Self-Stirrin
1700/1725 Brannum Lane Yellow Springs, Ohio 45387 USA (800) 765-4974 (937) 767-7241 FAX: (937) 767-9353 Website: http://www.ysi.com E-mail: info@ysi.
