3D Microscopy for Axiovert 100/135/135 M Operating Manual
Copyright 3D Microscopy Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change. Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted.
D Microscopy Contents Contents 1 1.1 1.2 1.3 1.4 1.5 2 2.1 2.2 2.2.1 2.2.2 2.2.3 2.3 2.3.1 2.3.2 2.3.3 2.3.4 3 Notes on safety ................................................................................................... 4 General.............................................................................................................................. 4 Regulations concerning instrument safety and electromagnetic compatibility (EMC)........... 4 Notes on unpacking, transport and storage........
Notes on safety 1 Notes on safety 1.1 General Please make yourself familiar with the contents of these operating instructions before attaching and starting up the components for 3D microscopy. Additional information is available from our maintenance service or from authorized agencies. For the function of 3D microscopy in combination with the Caution! Non-observance of the safety notes constitutes a hazard for the user.
3D Microscopy 1.3 Notes on unpacking, transport and storage Notes on unpacking, transport and storage The components for 3D microscopy must be transported in their original packaging. It is recommended to use the original packaging also for storage. The transport and storage temperatures must comply with those listed under "Technical Data". Cable connections may only be made if the various components are switched off.
General Application 2 General 2.
Function Eypiece version 3D Microscopy 2.2 Function The dynamic technique on which 3D microscopy is based can be used with any light microscope with an accessible aperture diaphragm. The technique can be used with upright or inverted reflected-light and transmitted-light microscopes. A patent has been applied for the technique. There are three different versions of 3D microscopy: eyepiece version monitor version combination of eyepiece and monitor version 2.2.
Function Monitor version 3D Microscopy 2.2.2 Monitor version An LCD shutter ("3D illumination shutter") is positioned close to the aperture diaphragm and is used to open or close half a side of the beam path. With the monitor version, the LCD shutter is controlled via the "3MCU II" unit. The centroid of the illumination beam path is switched in such a way that the object is illuminated alternately from a right and a left stereo angle.
Function Monitor version 3D Microscopy 1 2 3 4 5 6 7 8 Monitor shutter Glasses Control unit for monitor shutter MCU II image memory 3D illumination shutter Objective Object plane Camera Monitor version in a reflected-light microscope B 40-022-4 e 07/98 1 2 3 4 5 6 7 8 9 Monitor shutter Polarizing glasses Camera Objective Object plane Condenser 3D illumination shutter MCU II image memory Control unit for monitor shutter Monitor version in a transmitted-light microscope 9
Function Combined version 3D Microscopy 2.2.3 Combination of eyepiece and monitor version An LCD shutter ("3D illumination shutter") is positioned close to the aperture diaphragm and is used to open or close one half of the beam path. With the combined version, the LCD shutter is controlled via the "3D-CONTROL DSA-3" unit. The centroid of the illumination beam path is switched in such a way that the object is illuminated alternately from a right and a left stereo angle.
3D Microscopy 2.3 Maintenance and care Maintenance and care 2.3.1 General Never expose the components for 3D microscopy to inadmissible climatic conditions (high humidity and temperature) for longer periods of time. The components must be protected from dust and humidity and covered with the dust cover after every use. 2.3.2 Cleaning of optical components The 3D shutter in the eyepieces and in the 3D illumination shutter are equipped with a polarizing foil.
Troubleshooting 2.
3D Microscopy 3 Technical Data Technical Data Parameters Description Ambient conditions Operation Storage Transport Temperature + 10°C ... + 35°C 0°C ... + 35°C 20°C ... + 50°C Humidity max.
Contents 14 3D Microscopy Axiovert 100/135 B 40-022-4 e 09/98
3D Microscopy Axiovert 100/135/135 M Contents 3D Microscopy for Axiovert 100/135/135 M Contents Components for 3D Microscopy Overview .................................................. 16 Eyepiece version................................................................................................ 18 Converting the microscope .............................................................................................. 18 3D-CONTROL DSA-3 Control Unit .................................................
Overview 3D Microscopy Axiovert 100/135/135 M 1 Components for 3D Microscopy Overview Fig.
3D Microscopy Axiovert 100/135/135 M Overview Axiovert 100/135/135 M Eyepiece version Monitor version 4 3D illumination shutter 2 Eyepiece PL 10x/25 with 3D shutter Eyepiece PL 10x/25 with 3D shutter 5 3D-CONTROL DSA-3 3D-CONTROL DSA-3 3 LD condenser 0.55 Ph1 Ph2, PH3, DIC, DIC Cat. No. 451359 1 3D depolarizer Axiovert 100/135/135 M Centering telescope (Axiovert 100) Cat. No. 444830 6 7,8,9 10 11 12 3D illumination shutter Eyepiece and monitor version LD condenser 0.
3D Microscopy Axiovert 100/135/135 M Eyepiece version 2 Eyepiece version 2.1 Converting the microscope Set up the microscope in accordance with the operating instructions of the Axiovert 100/135/135 M Equip the microscope with LD condenser 0.55 Ph1, Ph2, Ph3, DIC, DIC Connect the components for 3D microscopy to the 3D-CONTROL DSA-3 control unit: Fig.
3D Microscopy Axiovert 100/135/135 M Eyepiece version Insert eyepieces with 3D shutter in the binocular tube Insert 3D illumination shutter in the recess provided in the condenser of the Axiovert 100/135/135 M, close to the aperture diaphragm Fig. 4 Do not touch the polarizing foil, since it can be easily scratched. 1 2 3 4 5 Stop screw (hexagonal, SW 2, varnish-coated) LD condenser Fixation screw (hexagonal, SW 2) Recess 3D illumination shutter Fig.
Eyepiece version 2.2 3D-CONTROL DSA-3 Control Unit 3D Microscopy Axiovert 100/135/135 M 2.2.2 Controls Fig. 6 STEP If the rotary switch is in the "STEP" position, this button can be used to change to statically inclined illumination for the left and right beam path. Fig. 6 3D-CONTROL DSA-3 control unit, front STEREO/PSEUDO Toggle switch which determines the phase position between the shutters.
3D Microscopy Axiovert 100/135/135 M 2.3 Adjustment Eyepiece version Use cover slip as an object and focus on it using the 10x objective; after adjustment, check the setting using the objective of the highest magnification 2.3.1 Adjustment of 3D illumination shutter Fig. 7 Move rotary switch of the 3D-CONTROL DSA-3 to the STEP position: Fig.
Eyepiece version 2.3.2 Check the contrast If colors or inhomogeneity are visible in the pupil, change the voltage settings for the 3D illumination shutter and the 3D shutter of the eyepieces on the potentiometers of the 3DCONTROL DAS-3 until optimum contrast and brightness are achieved.
3D Microscopy Axiovert 100/135/135 M Eyepiece version 2.3.3 Check whether image is upright Set the rotary switch of the 3D-CONTROL DAS-3 to 3D VIS Set the toggle switch of the 3D-CONTROL DAS-3 to STEREO View a familiar object; if the image is not upright, exchange the eyepieces Mark the eyepieces and the appropriate plugs with the supplied labels Fig. 8 E1 and E2 L and R Fig.
Eyepiece version 3 Monitor version The following components from KAPPA are required for the monitor version: MCU II control unit CF 15/4 MC-S camera CF 15/4 connecting cable to MCU II Line cable Control unit for monitor shutter, with power unit and connecting cable 3D illumination shutter Control and supply cable between MCU II and 3D illumination shutter/monitor shutter Polarizing glasses Monitor (100 Hz) with connecting cable and attached monitor shutter Fig.
3D Microscopy Axiovert 100/135/135 M 3.1 Monitor version Conversion Set up the microscope in accordance with the operating instructions for the Axiovert 100/135/135 M Equip the microscope with LD condenser 0.
Monitor version 3D Microscopy Axiovert 100/135/135 M Connect camera to phototube via instrument-specific video adapter or video zoom; the following adapters are recommended: Video zoom adapter 0.4x 2x with C-mount TV adapter 1x for chip camera with C-mount Fig. 12 1 2 3 Camera Adapter Phototube Fig. 12 Attachment of camera Push 3D illumination shutter in the recess provided in the condenser of the Axiovert 100/135, close to the aperture diaphragm: Fig.
3D Microscopy Axiovert 100/135/135 M 3.2 Monitor version MCU II Control Unit 3.2.2 Basic settings for 3D microscopy USER MENU RESET MENU White set Integration 1 2 3 4 5 Line switch Menu change Menu display (LCD) Storage key for current image Arrow keys for parameter control in the menu Fig. 13 MCU II controls, front 3.2.1 Controls Fig.
Monitor version 3.
3D Microscopy Axiovert 100/135/135 M 3.4 Monitor version 3D video recordings 3D sequences can generally be recorded and played back using a video recorder. This requires an S-VHS recorder and an S-VHS video tape. Connecting the video recorder: Set the toggle switch of the MCU II to Extern Fig.
Eyepiece and monitor version 4 Eyepiece and monitor version 4.1 Before connecting the components, first read the enclosed manual from Kappa entitled "Color TV Camera for Microscopy and 3D Images"; chapter 5, "Safety regulations", should be observed in particular. Fig. 15 30 3D Microscopy Axiovert 100/135/135 M Conversion and adjustment Set up the microscope in accordance with the operating instructions of the Axiovert 100/135/135 M Equip the microscope with LD condenser 0.
3D Microscopy Axiovert 100/135/135 M Eypiece and monitor version 4.2 Observation This is a combination of the eyepiece and the monitor version. The image is 3-dimensional when you look through the eyepiece or on the monitor. 4.2.2 Monitor observation 4.2.
Eyepiece and monitor version 32 3D Microscopy Axiovert 100/135/135 M B 40-022-4 e 09/98
