Specifications

Operation
Axiostar plus Transmitted-light darkfield Carl Zeiss
B 40-815 e 12/01 3-9
3.4.3 Setting transmitted-light
darkfield
Before starting:
– As described in section 2, the microscope is
ready for operation and switched on
according to section 3.1.
Settings:
• Set KÖHLER illumination in the same way as
for transmitted-light brightfield, e.g. with
objective 10x.
• Insert the relevant slider (3-8/3) for darkfield
− see table on page 3-8 − into the opening
on the condenser until stop.
• Open the luminous-field diaphragm (3-8/2).
• Increase the brightness setting on the
potentiometer.
• If the image center is too bright or too dark,
sensitively correct the condenser height
until the field of view appears
homogeneously dark or features
homogeneous brightness distribution.
For darkfield setting, it is also possible to use
the slider for phase contrast 3 and the
following objectives instead of the slider for
darkfield 0.25/0.9: CP-Achromat, A-Plan and
Plan-Neofluar 2.5x – 10x and Achroplan 4x.
Darkfield condensers can be placed directly in
the Axiostar plus when you use the darkfield
condenser carrier. Chapter 2 offers an overview
of applicable types and recommended
objectives.
☞
Darkfield specimens require a considerably
higher level of cleanliness than specimens for
other methods; fingerprints, dirt and dust in
particular brighten the background and reduce
the contrast of the object image.
1 Focusing drive
2 Luminous field diaphragm
3 Dark field slider
4 Aperture diaphragm lever
5 Centering screws for condenser
6Nosepiece
7 Binocular tube
Fig. 3-8 Setting transmitted-light darkfield